Examination of Bacterial Content of Sea Water

The following tests according to the lab manual were performed: gram stain, fermentation tubes, methyl red, vogues proskauer, sulfur, indole, motility and growing it up on MacConkey agar. The gram stain was performed incorrectly the first time. This is because the decolorizer was not on the bacterium slide for long enough, giving a false outcome.

2) Record the shape of the bacteria, the arrangement of the bacteria, and the gram staining characteristics.

In the Voges-Proskauer test, I inoculated the tube with bacteria from my TSA plate and incubated the tube at 37 degrees Celsius for 3 days. After three days I placed some Barrits Reagent A and Barrits Reagent B in the test tube. The color change of red or pink indicates a positive reaction for acetoin which tells you that the organism is a butanediol fermentor.

Pullulated coastal waters have health and economic repercussions. Excess water pollutants, mainly due to contaminated runoff, in beaches puts the health of residents and tourists at risk, which has a huge impact on public health, leading to economic loss in that regard. Also, the high incidence of water pollutants can deter people from visiting the

The first experiment determines the amount of bacterial colonies found in the creek water sample. The serial dilution method and the pour plate method will be used to minimise that the number of bacterial colonies to gain visible quantitative data. Two types of samples will be conducted for the first. One labelled full strength will contain 1ml of pure creek water and the second, half strength will contain 1ml of a mixture between creek and diluted water. Half strength and full strength refers to the starting amount of bacteria.

Different strains of E.coli can be isolated and identified by a variety of biochemical tests. The biochemical tests commonly used to identify E.coli include: lactose, oxidase, indole, and beta-glucuronidase tests. The use of lactose to identify a strain of E.coli is an appropriate method since intestinal bacteria, such as E.coli, typically undergo lactose fermentation. Using MacConkey agar, microbiologist are able to analyze if lactose is fermented and detected by the change of color in the medium. The oxidase test is used to identify if the bacteria synthesizes the enzyme cytochrome c oxidase that is part of the electron transport chain. When the enzyme cytochrome c oxidase is present, the reduced reagent, tetramethyl-p-phenylenediamine will donate its electron to cytochrome c oxidase and a purple color end product will show in the media. The indole test is performed by microbiologist to determine if E.coli is capable of converting tryptophan to indole. Lastly, beta-glucuronidase test is used to determine if an organism produces the enzyme glucuronidase by hydrolysis of nitrophenyl-beta-glucopyranosiduronic acid. In the presence of glucuronidase, the medium develops a yellow color (Trepeta & Edberg,

The first test I conducted was on the MAC agar because it could show the ability of the microorganism to ferment lactose. The neutral red indicator serves to contrast the lactose-fermenting from the non lactose-fermenting organisms. My bacteria remained translucent and the medium kept it color which means that the organism did not ferment lactose.

This study was done to determine unknown microorganisms that were retrieved from pond water in Rocklin, California. If the study involves identifying microorganisms from water, various amount of test are done to find what type of bacterias are in water. The most common bacteria in the genera Eschericha, Pseudomonas, Acinteobacter, and Aeromons are amongst the top bacteria found in water. As a result, one or more of these microorganism will be found in the pond water collected. It is important to specify one of these bacteria in the water sample as it is potentially harmful. Some strains of Eschericha Coli are known to cause “serve gastrointestinal infection, dysentry and even death in susceptible individuals”(Wilson, 2015). Most importantly, it is important to find these species that can cause danger to humans. So, they can be treated accordantly with the right antibiotics if they presented any of the side effects.

In order to detect any seasonal or spatial patterns in the prevalence and phenotypic diversity of Salmonella, E. coli, and Enterococcus species in surface waters, the phenotypic analyses of these bacteria will be conducted. Salmonella will be serotyped, E. coli will be phylo-grouped, and Enterococcus species will be determined using multiplex PCR methods 11, 12, 13. Common serotypes or species of bacteria from different seasons, years, or locations along the rivers and streams in the Upper Oconee watershed will be determined in order to find out if these serotypes and species are genetically related. E. coli will also be enumerated as an indicator of water quality.

This experiment consists of four methods, of measuring and weighing room temperature water using the different devices, each method repeated five times. Method one is to

During a recent diving trip to the Mariana Trench, The Unmanned Submarine ‘Aishiteru’ collected several water samples that were highly concentrated with unknown microbial communities. Therefore our team need to figure out how the water samples should be transported to the nearest advanced microbiology laboratory to be analyzed. The microbes have to be survived during the long journey.

Now that we know how the bacteria survives, we will discuss the basic components of its environment. It was first discovered in sediments found in the North Sea, where there is an abundance of oil and gas wells. A. borkumensis is found naturally in seawater. While A. borkumensis has been found in non-polluted water, it is most commonly found in oceans that contain petroleum oil. This oil can be found naturally or from oil spills. It is found in

Examination Of The Different Possible Microbes Present In The Water System Prior To Purification & Potential Damages Without Water Purification

Antimicrobial resistant bacteria in seafood are a major public health concern worldwide. Reports on use of antimicrobials in aquaculture and residue findings in the products have indicated food safety threat to the consumers. In aquaculture, the use of wide array antimicrobials leading to development of bacterial resistance, use of resistant probiotic resistant strains and contamination of resistant pathogenic bacteria e.g. Salmonella spp. as input have facilitated to development of pool of resistant bacteria. Indicator bacteria are a useful tool to monitor antimicrobial resistance in any animal originated products. E. coli and Enterococci have been proven useful bacterial indicators in livestock and other animal husbandry practices

To determine the viable bacterial numbers in the sputum sample, the sample is homogenized with dithiothreitol and glass beads in order to determine the method which recovered the greatest number of viable bacteria. Continuous monitoring systems of mycobacterium report higher contamination rates than traditional radiometric technologies. The first step in analyzing fresh sputum is the gram stain test which seeks to identify the type of bacteria in the sample. If the sample is seen to contain