Exercise 1

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Activity 1: 1. Did any solutes move through the 20 MWCO membrane? Why or Why not? 2. Did Na+Cl- move through the 50 MWCO membrane? Activity 2: 1. Are the solutes moving with or against their concentration gradient in facilitated diffusion? 2. What happened to the rate of facilitated diffusion when the number of carrier proteins was increased? 3. In the simulation you added Na+Cl- to test its effect on glucose diffusion. Explain why there was no effect. Activity 3: 1. Which membrane resulted in the greatest pressure with Na+Cl- as the solute? Why? 2. Explain what happens to the osmotic pressure with increasing solute concentration. 3. If the solutes are allowed to diffuse, is osmotic pressure generated? 4. If the solute…show more content…
Do you think urea will diffuse through the 20 MWCO membrane? Your answer : c. No, not at all. Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with 607 amino acids, and the average molecular weight of a single amino acid is 135 g/mole. Which of the following will be able to diffuse through the 200 MWCO membrane? Your answer : c. glucose only Stop & Think Questions: The reason sodium chloride didn't diffuse left to right is that You correctly answered: c. the membrane pore size was too small. Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average molecular weight of a single amino acid is 135 g/mole. There is no reason to run these solutes at the 20 MWCO because You correctly answered: d. glucose and albumin are both too large to pass. The rate of diffusion for urea You correctly answered: b. is slower than that for sodium because urea is a larger molecule. Experiment Data: Solute Na+ ClUrea Na+ ClNa+ ClNa+ ClUrea Glucose Albumin MWCO 20 20 50 50 100 100 200 200 Solute Concentration 9.00 9.00 9.00 18.00 9.00 9.00 9.00 9.00 Average Diffusion Rate 0.0000 0.0000 0.0150 0.0300 0.0150 0.0094 0.0040 0.0000 Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. The effect of increasing the concentration of sodium chloride from 9 mM to 18 mM in the left beaker was to You correctly answered: b. increase the rate of diffusion. 2. Describe the difference

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