Exercise 3 Biology Answers

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DISCUSSION
A. Was your initial hypothesis supported or refuted as to which test tube starch contents would convert to sugar first? Yes, my initial hypothesis was supported. I thought the test tube with the highest concentration amylase would convert first and it did.

B. What have you learned from this experiment?
I learned that the higher the concentration of amylase enzyme the faster the starch will convert to sugar. However, I learned that in time the test tubes would change due to the amylase in many different ways. Meaning, the quickness is not the only thing that affects the change of starch to sugar.

C. What practical applications could this knowledge have?
Sugar can be helpful for yeast growth when it is turned into glucose
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Describe another way to do this experiment that would test the same concept.
Instead of changing the amount of amylase enzyme added to the test tubes, change the amount of starch added to the test tube. Meaning, the amylase will already be in the test tubes and the starch will added into the test tubes at different amounts. The rest of the experiment would be performed in the same way.

Exercise 3: Enzymes and pH
QUESTIONS
A. What is the variable in this experiment?
The different pH level buffers is the variable in this experiment.

B. In which test tube(s) did the amylase convert starch to sugar? Why might you expect this outcome?
Test tube 3 and test tube 4 the amylase converted starch to sugar. The higher buffer levels allowed the amylase to convert starch to sugar.

C. Did any test tubes NOT exhibit a color change? Why?
Test tube 1 and 2 didn’t show any color change. These two test tubes had lower levels of pH. Therefore, the enzymes could not change the starch to sugar.

DISCUSSION
A. Did the results support or refute your hypothesis as to which test tube starch contents would convert to sugar first?
It was supported.

LABORATORY SUMMARY
A. What other conditions that may affect the action of enzymes? Substrates could affect the action of the

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