Experiment 6 Petri Disheses

The human race is about to be annihilated in 240 hours and at the bottom of it all is an infamous genius. Equipped with vast resources stolen from the Supreme Leader of North Korea, the most powerful man on the planet decides to impart his wisdom.

In which petri dish did you observe the highest hatching viability? Did the results support your prediction in Pre-Laboratory Question #2?

* Aprons must be removed and discarded appropriately. Hands should then be washed and dried or decontaminated with alcohol handrub.

Wash your hands and all of the equipment with mild soap and water. As a final rinse on the equipment, use deionized water. Dry all equipment thoroughly.

Read in your lab manual about the following agar mediums: Blood Agar (pg 168), EMB Agar (pg 170), Mannitol Salt Agar (MSA)(pg 172) ), MacConkey Agar (pg 174), and PEA Agar (pg 176) to answer the following:

Note: For experiments one through three, each petri plate should be taped shut and stored upside-down for one week at room temperature to incubate the

In part II of the lab six small glass tubes were obtained in a test tube rack. Ten drops of distilled water were then added to test tube 1, five drops to tubes 2-4, and no drops in tubes 5 and 6. Five drops of 0.1M HCl were added to test tube 5 and five drops of 0.1M NaOH to test tube 6. Five drops of enzyme were then added to all tubes except tube 1. Tube 3 was then placed in the ice bucket and tube 4 was placed in the hot bucket at 80-900C for five minutes, the remaining tubes were left in the test tube rack. After the five minutes five drops of 1% starch was added to every tube and left to sit for ten minutes. After ten minutes five drops of DNSA were then added to all the tubes. All the tubes were then taken and placed in the

Some brainstorming was done to determine the lineup of the bacteria on two Petri dishes. Next, the experiment started with the labeling of the two Petri dishes. This was followed by a mistake that occurred, which was corrected with an orange dry-erase marker. Then our hands were sanitized with hand sanitizer, and was followed with bacteria collecting. The bacteria was collected, from the silver-ish, doorknob on the inside of the door near room B216 (with evacuation plan), and the beginning part of the handrail at the right found near the stairs of the B building opposite to Luck Hall. After that, the process was

Wash hands, place on lab coat, and disinfect work area with the ten percent bleach.

After a surgery has taken place, laboratory technicians will soak and wash the used equipment in cold water mixed with Gain antibacterial soap. Once scrubbed with soapy water, the technician rinses the clean instruments under cold water to remove the suds. Next, they will lay the tools out on a small towel in order for them to dry. While drying, the technician will walk to the back of the clinic to cut a new drape.

Professor Weinberg load up three petri dishes with different compounds: tetracycline, bacteria, and an elemental nutrient. During his experiments, he noticed that the bacteria did not stop to growth, so he deduced that a nutrient was providing so much booster fuel, in this case iron. However, to be valid the results, first I would put on a dish too much iron, then add the bacteria and the antibiotic. As a result, I would expect that the high levels of iron lead to the highly increase of bacteria, and moreover, the antibiotic did not work at all. Consequently, I would put few iron in a dish, then add the bacteria and the antibiotic, therefore, as I expected, the low levels of iron did not permit the growth of bacteria, and the antibiotic was

For this experiment we utilized a variety of materials. We first utilized a sharpie pen to write down the locations of our experiments and our names among other information on seven different Agar plates. We then used seven different containers of sterilized water to make sure there was no contamination between different samples. We also utilized seven different disposable swabs for this exact same reason. We also used seven packages of Para-film in order to seal the plates. Lastly we made use of a freezer in order to keep our plates at the proper temperature for bacteria incubation and growth. These are all the physical materials we made use of while conducting our own experiment.

Laboratory ABC uses both glass pipettes (1mL to 5mL) and disposable plastic pipette tips- yellow (20μL), blue (1000μL) and white (5mL). Glass pipettes and white pipette tips are currently washed and reused. Blue and yellow tips are discarded.

Two petri dishes with glucose and nitrogen rich mediums were each inoculated with cells obtained from different locations within the Biology building using aseptic techniques: the sink handles located on the left side of the lab room and the lab floor. An additional two petri dishes with mediums were obtained in order to purposely engage in poor laboratory techniques. One petri dish was left open for approximately an hour and a half and the other petri dish was touched by each group member’s finger. The four petri dishes were placed in an incubator set at 37°C for a week to allow for sufficient bacterial growth (Holbrook and Leicht 2017).

- Have in place a broom and dust pan to clean around the demo area