Experiment to Study the Importance of Rubisco Carboxylase in Plants

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RuBisCo is an enzyme involved in the first major step of carbon fixation. Three tobacco plants had three differing levels of ruBisCo carboxylase. With these differing levels came various levels of performance in terms of oxygen and carbon dioxide output. Perhaps this is why the carbon dioxide uptake was lower than the net evolution of oxygen in the third plant with 10% rubisco carboxylase. It appears from these measurements shown in figure 2 of the tobacco plants with reduced amounts of rubisco that photoreduction of oxygen cannot advance in the nonappearance of ATP consumption correlated with chloroplast carbon metabolism. The cause of this might be "the strong controlling influence of the thylakoid lumenal pH on regulation of electron flow through the bf complex" (Laisk et al., 1997). In terms of having the greatest output of carbon dioxide and net evolution of oxygen, the second plant with the 40% level of rubisco carboxylase was the highest. It was the second plant that reached almost forty with the first plant never getting past thirty and the third plant barely getting to ten. It seems 40% level of rubisco carboxylase offered the second plant peak operating performance. This is the key difference from plant one and plant two. They share similar growth rates in the charts and allow for close to the same levels of performance, but differ in the efficiency of said performance. In terms of plant metabolism, there is slightly more carbon dioxide absorbed in the tobacco

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