Explanation Of Bubbles Lab Report

The purpose of this lab was to identify an unknown bacteria culture using differential tests. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. It is suggested that culture 11 is a sample of Enterobacter aerogenes.

Using a thermometer we observed the measurement of the water “H2O” in the beaker, and it resulted in an initial temperature of 20 ⁰ C.

Lastly, the water that the beakers were washed with may have steadily lowered the temperature of the glass throughout the trials and therefore affected the base temperature of the vinegar and the temperature of the

gas bubbles serves as evidence that the catalase enzyme is working. As catalase is breaking the

Before boiling commenced two layers formed in the reaction flask. The bottom layer was slightly cloudier than the clear top layer.

My unknown organism #6 is Morganella morganii, which is a gram-negative bacillus rods commonly found in the environment and also in the intestinal tracts of humans, mammals, and reptiles as a normal flora. (3, 5) This bacterium Morganella morganii, was first discovered in the 1906 by a British bacteriologist named H. de R. Morgan. (2) Despite its wide distribution, it is an uncommon cause of community-acquired infection and is most often encountered inpostoperative and other nosocomial settings. (2, 3) Morganella morganii infections respond well to appropriate antibiotic therapy; however, its

The purpose of this lab was to identify unknown bacteria cultures using various differential tests, and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were Gram stain, Catalase, Mannitol Salt Agar (MSA), Blood Agar, Novobiocin, Coagulase, and DNAse (Alachi, 2007).

Bacteria are ubiquitous; they can be found on the skin, in the soil, and inside the body. Because of the very nature of this ubiquity, it is important to be able to determine between different strains of bacteria. An example of this is determining the causative agent for a disease so that the patient will be treated with the appropriate antibiotics. It may be important to determine the bacteria in a certain region, because like with enteric bacteria, it is normal to find them in the digestive tract as they are in a symbiotic relationship with our bodies in this area; however, they also cause opportunistic infections in places outside of the digestive tract to our detriment, such as with a urinary tract infection. Some strains of bacteria are common to nosocomial infections, and identifying these bacteria as such helps create the guidelines for healthcare workers in antiseptic technique. All of the morphology and characteristics of each strain of bacteria help us to better understand the role of bacteria in the body as well as helps us understand how they can cause illness, and what treatment regimen to set in place. In lab this semester, a sample of unknown

Identifying Organism 6C Introduction: This report details the steps taken and processes used to discover the identity of the unknown organism given to me on Tuesday, November 28th, 2017. With all of the knowledge and skills gained over the semester, in class and in lab, I was able to positively identify my unknown organism. The objective of these labs being, that I successfully utilize the tests and procedures taught during the course to correctly identify my organism and to be able to explain the reasoning behind my tests and results.

Triple Sugar Iron Agar test, there was a gas production seen in the media. The media was yellow slant and yellow butt indicating glucose, lactose and/or sucrose fermentation with acid accumulation in slant and butt. For sulfur reduction, it was negative since it did not turn black in color indicating no sulfur was reduced.

This is because as the temperature increases, the water molecules start moving faster and faster- which leads to more collisions between the water molecules and the Alka-Seltzer molecules. The Alka- Seltzer dissolved fastest in the 110° water. When dissolving, the Alka-Seltzer produced a white fizzing substance. When the Alka Seltzer dissolved in higher degrees, the Alka dissolved completely into water. However in the lower temperatures, the Alka Seltzer dissolved but left gas bubbles at the bottom of the beaker.

From the results that were acquired from mixing the liquid reagents with each powder, it was determined that Unknown Mixture #1 consisted of baking soda and cornstarch. When individually testing the substances from Unknown Mixture #1 with the liquid reagents, a few noticeable reactions occurred. Mixing baking soda with vinegar caused bubbling to occur. This is because a neutralization reaction took place between the two reactants. In this reaction, sodium bicarbonate(baking soda) reacts with vinegar and produces sodium acetate, water, and carbon dioxide(HC2H3O2(aq) + NaHCO3(aq) NaC2H3O2(aq) + H2O(l) + CO2(g) ). The gaseous carbon dioxide most likely tried to escape into the atmosphere and caused the bubbling to occur. Another noticeable reaction

An unknown was given to our group from the professor. The unknown was in nutrient broth, the group received unknown number 3. And the task was to identify the unknown and try to make an educated guess, and identify the unknown #3.

8. In order confidently determine what substance my “G9R” was I would have to do over the boiling point experiment a couple of more times. I would turn the gas off and take the Bunsen burner away from the apparatus when the stream of bubbles started coming out from the mouth of the capillary tube. This would allow me to correctly determine when the atmospheric pressure was equal to the vapour pressure.

Osmosis is when water passes through a cell membrane, it is also form of a diffusion, which is a form of passive transport. Osmosis will continue to until an equilibrium is reached which is when the solutions are isotonic. This means that the solution has the same amount of solute on both sides. If the solution is hypertonic, it has more solute in the solution. In this situation water will move towards it. if the solution is hypotonic, it has less solute in the solution. Whereas in this situation, water will move out of the solution.