Extraction of Caffeine from Tea Leaves

According to the results, the columns of caffeine in figure 1, of this experiment the hypothesis for caffeine is partially accepted. There is an increasing trend in the change of pulsation rates with increasing

TLC Analysis Data Table Compound Std. Aspirin Std. Caffeine Rf values 0.38 0.65 Error ---~ 0% 29% 5%

Many manufactures release the caffeine content of their products publically, but not always, and new products and flavors are continuously introduced to the market. If quality checks are not performed, manufactures may alter the caffeine and benzoic acid content to suit the demands without public knowledge. To ensure the levels of caffeine and benzoic acid in products do not exceed the established safe limits and to inform the public of the amount of these compounds being consumed, various methods of analysis have been performed. Before the introduction of modern techniques, spectrophotometric methods alone were used to determine concentration of a compound in a mixture.6 The caffeine content in coffee, tea, soft drink, and energy drinks were determined using an immunoassay.7 The caffeine content in mixtures also used to have to be extracted before quantification.8

← I would repeat the experiment with the caffeine solution more times, so that the results I would get will be more

Caffeine is a wildly use drug in today’s society. Caffeine is a methylated xanthine which acts as a mild central nervous system stimulant (MS & RL, 2001). It is a stimulant which acts upon the central nervous system and increases alertness, wakefulness and restlessness and it increases the release of catecholamine from renal medullar (Fernandez, 2016; Collines, 2007). It is present in many beverages. Caffeine is found in coffee, tea, soft drinks, products containing chocolate (cocoa) and some medication (Collines, 2007). It is used as a cardiac and respiratory stimulant (Collines, 2007).Caffeine is the most frequently ingested pharmacologically active substance in the world (Collines, 2007).

Table 1 – The body temperature of the test subject after being exposed to 50 mg of caffeine.

Caffeine is a mild stimulant that occurs naturally in at least 63 plant species. Caffeine can be found in the leaves, stems, seeds, and roots of these various plants. Caffeine is part of the methylxanthine family. It consists of a xanthine molecule with three methyl groups attached to it. Caffeine can be found in many products like sodas, tea, and coffee, but it also occurs in several other products such as prescription medications, diuretics, and pain relievers. Caffeine’s widespread use and popularity have caused many people to view the substance as an addictive drug. Thus making caffeine the most inexpensive and readily available drug known to man. Then on the other hand there are

5.    Prepare the caffeine solution by dissolving 10g of caffeine tablets in 100ml of water in a beaker. Label the beaker ‘caffeine’. Similarly, add 10g of coffee to 100ml of water in another beaker and label it ‘coffee’.

An independent samples t-test was conducted to examine the difference between experimental conditions on test performance. The results indicated a significant difference between participants who consumed the caffeinated beverage and participants who did not, with participants in the caffeinated group (M = 7.64, SD = 2.41) performing worse than participants in the non-caffeinated group (M = 9.81, SD = 3.16), t (97) = 2.14, p < .05.

Caffeine is a natural product that is extracted from the raw fruit of coffee plants over sixty creased alertness. Caffeine gives most people a temporary energy boost and elevates mood..species to be exact. kola nuts, cocoa, yerba maté, guarana berries, coffee beans and teas contain this. Caffeine is rapidly absorbed in about 30 to 60 minutes in our bodies after ingestion. Caffeine is defined as a drug because it stimulates the central nervous system, causing increased alertness. Caffeine gives most people a temporary energy boost and elevates mood.caffeine can also be in things like over the counter medications, and pain relievers.

In this experiment, the purpose of this experiment is to isolate pure caffeine from tealeaves. Caffeine does not exist alone within the tealeaves, but other substances that are in the leaves itself accompany it. There are major and minor products that make up the constancy of the leaves and with those substances they need to be separated out of the leaves. The main component of the leaves are, cellulose and polymer of glucose, while the minor components of the leaves are caffeine, tannis, flauaniods and chlorophylls. By weight, caffeine is 5%, which is an alkaloid and contains C, H, N, O; it is very soluble in water and is the main component that will be extracted from the experiment. Although caffeine is very soluble in water it is much more soluble when it is dissolved in methylene chloride. Therefore, when completing the methylene chloride process, it will nearly purify all of the caffeine, making it pure caffeine. Make sure not to breathe in the fumes of the chemicals or spill them on yourself. Safety precautions

Components containing caffeine were composed into stock solutions. These solutions were diluted to 1: 10 substance: mobile phase. A stock solution of caffeine was diluted 1:50. A sequence of diluted caffeine solutions were prepared for use as a standard (ppm): 1, 2, 4, and 10. Solutions of acetaminophen, acetylsalicylic acid, and Goody’s Powder were developed to differentiate chromatographic peaks observed. These solutions were subjected to HPLC for examination of the observed peak area and retention time for the set of compounds. Comparison of retention time allowed for the differentiation of peaks observed. The peak area obtained was utilized to determine the relative concentration of caffeine present in Goody’s Powder based on the relationship obtained in the standard. The content of caffeine present in Goody’s Powder by percent weight was identified.

First, saturate the TLC trough with 30 ml of ethyl acetate: methanol solution for 10 mins. Next, spread the silica gel into into a 0.2 micrometer film over the Aluminum plate. Then, to dry the plate for usage, heat the plates in an oven at 110 Celsius for 5 min. Apply the samples 1 cm above the base of the plate using a sample applicator, along with nitrogen gas to allow the bands to simultaneously dry. Place the TLC Plate into the chromatography trough, and allow the plate to develop until the 10cm mark. Follow up by scanning the TLC plate in a densitometer to obtain a chromatogram of the separated samples. The Peak of caffeine observed on the chromatogram can be matched to the real plate and be scraped off for the retrieval of caffeine.

Many of us find ourselves drinking a freshly brewed cup of coffee, an ice cold soft drink, a hot cup of tea, or an energy drink whether it be in the morning, afternoon, or in the night but one thing is that they all share a common ingredient: Caffeine

Many of us find ourselves drinking a freshly brewed cup of coffee, an ice-cold soft drink, a hot cup of tea, or an energy drink whether it be in the morning, afternoon, or in the night but one thing is that they all share a common ingredient: Caffeine