Factors Affecting the Rate of Catalase Activity

3090 WordsFeb 9, 201313 Pages
Factors affecting the rate of catalase activity The extent at which environmental factors affect the rate of catalase activity was discovered in this lab. The assay system, in which a filter paper disc was dipped into the enzyme and submerged using a stirring rod in a test tube filled with 20mL of hydrogen peroxide, was used to test several enzyme factors. As the saturation of hydrogen peroxide increased the rate of reaction increased as well. When the enzyme concentration increased the rate of catalase activity increased too. When catalase was subjected to an increase of temperature changes, the rate of reaction increased. Once the protein denatured around 100ºC the catalase activity decreased. Catalase operated with a high efficiency…show more content…
Add 20ml of 3% H2O2 to a test tube 2. dilute catalase 50u/mol: 2.5 ml 100u/mol catalase and 2.5 ml of H20 3. use tweezers to dip paper disc into catalase 4. place the paper disc onto the stirring rod and submerge to the bottom of the test tube 1. Add 20ml of 3% H2O2 to a test tube and measure depth 2. dilute the catalase 20u/mol: 1ml 100u/mol catalase and 4 ml of H20 3. use tweezers to dip paper disc into catalase 4. place the paper disc onto the stirring rod and submerge to the bottom of the test tube 1. Add 20ml of 3% H2O2 to a test tube and measure depth 2. use tweezers to dip paper disc into H20 3. place the paper disc onto the stirring rod and submerge to the bottom of the test tube Part C: Temperature (assay technique) 1. create a water bath of 0ºC, a room temperature bath 23ºC, 37ºC, and boiling water bath 100ºC 2. place 5ml of catalase at 100u/mol into 4 test tubes and set one in each water bath 3. allow test tube to sit for 5 min in the respective water baths 4. add 20ml of 3% hydrogen peroxide to 4 test tubes 5. used tweezers to dip 4 pieces of filtered paper discs into separate catalase baths 6. place disc on the bottom of the stirring rod and submerge each filter paper into a hydrogen peroxide test tube Part D: pH (assay technique) 1. add 3% 20ml hydrogen peroxide to 3 separate test tubes 2. diluted catalase using 0.1 HCL and 0.1 mol NaOH to pH 1, 7, and 11.5 3.
Open Document