Factors That Affect Enzyme Activity

1464 Words Oct 15th, 2014 6 Pages
CONDITIONS AND FACTORS THAT AFFECT ENZYME ACTIVITY

INTRODUCTION Catalase is an enzyme found in mammalian tissues and catalyzes the reaction by which hydrogen peroxide (H2O2) is decomposed into oxygen (O2) and water (H2O).(4) Enzyme activity and efficiency is affected by the conditions that the reaction occurs in. Changes in conditions can result in altering of the enzyme’s three dimensional shape and affect the enzyme’s function.(1) The conditions that influence the activity of an enzyme include pH, temperature, substrate concentration(s), ionic strength, and nature of salts present.(2) At a constant set enzyme concentration, the reaction rate increases with increasing substrate concentration until all available enzyme in bound to the substrates, at that point adding more substrate will not further reaction rate.(3)

PURPOSE
The purpose of the experiment conducted was to investigate the effects of various factors and conditions such as pH, temperature, substrate concentration and enzyme concentration on rate of chemical reactions.

HYPOTHESIS
It was hypothesized that the catalase enzyme would decrease in activity after a significant change in temperature, pH, substrate concentration and enzyme concentration from the optimal range of conditions. There are specific conditions in which the enzyme, catalase, will be most effective and have the fastest reaction rate and conditions where the reaction rate will be the slowest. The hypothesis states that the optimal conditions…
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