Factors That Affect The Activity Of Amylase

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Introduction According to Oxford Dictionary, metabolism is the set of chemical processes that occur within a living organism in order to maintain life. From these processes, organisms are allowed to develop and reproduce, maintain their structures, and respond to their environments. Metabolism is usually divided into two categories, which are catabolism- breaks down large molecules and anabolism- uses the energy to construct components of cells. The role of metabolism is to deploy starch or lipids stored in endosperm so the germination can take place. Thus, the energy was formed (catabolic) and new plant material as the seed germinates was synthesised (anabolic). The aim of the experiment was to enucleate amylase from barley and utilizing it for the catalyst of a biochemical reaction thus investigating throughout seed sprout the activity of amylase. Before the experiment, it is hypothesized that the higher the activity of amylase, the higher the sprout rate of seed. How high and radicle change in length can be used to observe this case. Therefore, when Benedict’s test is carried out, the solution is still blue for the inactive seed and the concentration of brick-red precipitate plants growing from seeds. Method To prepare for extracting amylase from germinating barley seeds, used mortar and pestle to grind 10 grains into a fine paste and added 10ml of buffer, to enucleate the amylase into solution. Then filtered the extract into a 100ml beaker, and recorded the volume. 5 ml
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