Factors That Affect The Rate Of An Enzyme

1827 Words Dec 11th, 2015 8 Pages
Abstract: This investigation presents what would occur to the reaction rate of an enzyme if the temperature, pH level, concentration of a substrate and enzyme were changed. In this experiment, the enzyme catecholase, commonly found in plants, was tested and its product formation, benzoquinone was measured. When the concentration of the reactants such as the enzyme catecholase, and the substrate catechol were tested, the results of product formation increased greatly because the higher concentrations allowed the enzymes to increase their activity rates. When physical factors such as temperature and pH were tested the results fluctuated.

Introduction: Enzymes are molecular catalysts used in biology to speed up chemical reactions. They are found in all organisms and they are important because they are necessary for cells to function, and without them reactions would be too slow to support life. Enzymes function by lowering the activation energy of chemical reactions, thus causing the reaction to speed up. Enzyme-catalyzed reactions are composed of the enzyme itself, substrates and products. The enzyme used in this particular experiment was catecholase which is an enzyme that is found in many plants. A substrate is the reactant molecule that is changed by the enzyme, and in this experiment the substrate used was catechol. Substrates and enzyme active cites bind together to form an enzyme-substrate complex, this is where the enzyme speeds up chemical reactions involving the…
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