Factors That Influence Catalase Activity

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Factors That Influence Catalase Activity Sumeet Salvi 11/16/12 AP Biology Block 3 Purpose: The purpose of this experiment was to test and observe the numerous amounts of factors that influence the activity of the enzyme and that can be done by releasing oxygen gas in a fixed condition and change conditions one at a time. Hypothesis: If a protein is acquainted with a disadvantageous condition that could denature the protein, then it will not release as much Oxygen gas as it normally should. Results/Data: Part A: The Time Course of Enzyme Activity Time (30 Second Intervals) | Gas Levels | 0:30 | 76 | 1:00 | 77 | 1:30 | 79 | 2:00 | 82 | 2:30 | 90 | 3:00 | 94 | 3:30 | 96 | 4:00 | 98 |…show more content…
This happened because there were some active sites that were empty, so the substrate was able to bind to them, but they slowly started to diminish, which caused the enzyme activity to vanish. In Part C of the lab, we repeated the same process as we did in Part A, but we used 3 different temperatures: 5oC, 37oC, 100oC. After we did the experiment for 5oC, it can be concluded that the enzyme was slightly inhibited because it started out at a gas level of 56, but at the last interval, it had a gas level of 60. That means it has not produced as much as it should have because the enzyme usually prefer to work in warmer environments, but if it is in a cold environment, then it will not produce as much as it should. After we did 37oC, it can be concluded that this was the optimal temperature of the yeast catalase because the gas level originally started at 11, but as time went on, the gas level started to increase a numerous amount and at the last interval, the gas level was 67. After we did 100oC, it can be concluded that the enzyme was almost entirely denatured because the gas level stayed at 0 for 5 intervals long, and when it increased, it barely increased. That happened because the enzyme has a melting point to which the enzyme functions and I believe the enzyme met its melting point because it could not produce nearly as much as it should have. In Part D of the lab, we
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