Feasibility Of Computer Aided Identification Of The Plasmodium Berghei Parasite

2187 WordsNov 15, 20169 Pages
Surya Krishnan Mrs. Gonzalez SRD 7th/8th 11/07/2016 Research Proposal I. Introduction A. Title Feasibility of computer-aided identification of the Plasmodium berghei parasite in the liver stage life cycle and the classification of the parasite phenotype B. Abstract Is it possible to find parameters that will allow the computer to identify if a parasite is infectious or noninfectious? C. Table of Contents I. Introduction I.A. Title I.B. Abstract II. Administrative Section II.A. Personal Statement (See VII.B.) II.B. Resources III. Specific Aims III.A. Significance and Innovation III.B. Hypothesis/Specific Aims IV. Research Design/Methods IV.A. Materials IV.B. Methods IV.C. Overview of design IV.D. Plans for measurement/data collection IV.C. Statistical analysis IV.E. Quality control and data management IV.F. Expected outcomes V. Ethics VI. References VII. Appendices VII.A. Glossary VII.B. Personal Statement II. Administrative Section A. Personal Statement See Appendix B B. Resources GFP-expressing Plasmodium berghei sporozoites were obtained by disruption of the salivary glands of freshly dissected infected female Anopheles stephensi mosquitoes. Human hepatocellular carcinoma cells (HepG2) were cultured and maintained at 37°C with 5% CO2 in MEM with Earle’s Salts media (Gibco-Thermo Fisher Scientific), containing 1% L-glutamine, 1% penicillin/streptomycin and 10% heat inactivated fetal calf serum. For infection, the HepG2 cells were

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