Fermentation Lab

Yeast Concentration and the Rate of Fermentation Experiment #1- Scientific Report Courtnie Niederbrach BIO L- 110 DR. VANPUTTE 10-15-14 Introduction There are many substances that can be manipulated and cause the rate of reaction in fermentation to either speed up or slow down. Substances that alter the rate of the reaction could be temperature of the water, the yeast concentration, pH, and the glucose concentration. In the experimental group of the experiment the amount of yeast concentration was manipulated. The objective of this experiment was to determine what factors affect the rate of the fermentation. To test this objective we changed the amount of yeast being used. A higher yeast concentration replaced the controlled yeast amount. A prediction made by my group was that higher amount of yeast would speed up the process of fermentation. Our null hypothesis is there will be no

1B). The tube with germinating peas had the heights rate of oxygen consumption. The reasoning behind this is because the peas were doing aerobic respiration in which the oxygen was consumed by the peas and the carbon dioxide was released from the peas. The thermobar tube had the lowest rate

Effects of Temperature on Anaerobic Fermentation in Saccharomyces cerevisiae. Abstract For the experiment, the changes of temperature on anaerobic fermentation the process in which cells undergo respiration without oxygen in Saccharomyces cerevisiae was observed. The purpose of this experiment was to test the effect of four different temperatures on the rate of carbon dioxide production in yeast by measuring the fermentation rate. Saccharomyces cereviviae, also known as Baker 's yeast, is a unicellular, eukaryotic sac fungus and is good for this experiment because of its characteristic of alcohol fermentation. It was hypothesized that fermentation increases with increased temperature to a point of 37°C; above that point, enzyme denaturing will occur and fermentation will decrease. The group was able to document the carbon dioxide production and mark each of the temperature intervals which were tested at temperatures 4°C (refrigerator temperature), 23°C (Room temperature), 37°C (Human body temperature) and 65° Celsius (Equal to 150°F). The experiment was conducted by pouring yeast solution with 2% glucose in fermentation tubes, placing the tubes in the appropriate incubation temperature, marking the rise of the gas bubbles in the fermentation tubes which indicated carbon dioxide production. The results of this experiment were not supported by the hypothesis, creating different results from what was predicted. It is important to understand the fermentation rate of yeast so

Triple Sugar Iron agar slant (TSI) was used to test for the fermentation of glucose and lactose, as well as the production of H2S. Gas production was also monitored, looking for fissures produced by production of gas during fermentation. The conversion of the originally red slant and butt of the agar to yellow indicates that

Abstract PH can affect the way fermentation occurs due to the irregularity of the acidity or alkalinity within the glucose solution. This is an enzyme-based reaction that is susceptible to pH. The aim of this experiment was to determine how pH affects the yeast fermentation rate by performing the experiment numerous times with a different pH of glucose solution which included pH 3, 5, 7, 9, 11. The hypothesis was ‘If the pH is lower than the neutral point then the fermentation reaction will occur faster?’ The experiment conducted was to measure the amount of C02 produced by the yeast going into fermentation, however varying the pH of glucose solution by using different pHs . To test this every 5 minutes the volume of gas in the test tube was observed and recorded until a period of 30 minutes had been. The end results

Fermentation Lab Jocelyn Birt 9/29/17 Fermentation Lab Abstract: This lab’s purpose was to see how different levels of yeast, distilled water, and sugar interact to affect the level of carbon dioxide evolved in fermentation. In this experiment we had two sections. The first section tested four test tubes with varying levels of yeast, glucose and distilled water for evolved carbon dioxide levels. The tubes were timed for 20 minutes. The amounts of solution in the test tubes are noted in the methods section of this lab report. The second section of the lab used three test tubes and flowed the same procedure except added spices. The levels of ingredients are also in the methods section. The main goal of this experiment was to see the effects of yeast concentration.

Alcoholic fermentation: C6H12O6  →  2CO2  +  2C2H5OH  +  2 ATP Lactic acid fermentation: Plant and fungal cells produce alcohol as a result of fermentation and animal cells produce lactic acid

The Effect of Sugar Concentration on Rate of Fermentation Aim: To investigate how concentration of sugar affects the production of carbon dioxide. Hypothesis: If the sugar concentration increases, then the amount of carbon dioxide produced will increased. Variables: The independent variable is sugar concentration. This will be changed by using solutions with different amounts of sugar (2%, 6%, 10%).

The Effect of Yeast Mass on the Rate of Glucose Fermentation by Yeast - A Practical Report SACE ID: 532883T BV Hypothesis: If the mass of yeast (g) is increased the rate of fermentation of glucose (mL/s) will increase. Independent Variable: The mass of yeast in solution (g). Dependent Variable: The volume of

The substrate is the hydrogen peroxide The product is H2O and O2. The rate of reaction will be measured by timing how long it takes for the spheres of yeast to sink and float up to the top again.

Beyond the microbrewery, a growing number of households are using fermentation techniques that preserve fruits and vegetables all year long, without the use of electricity. Long before the advent of refrigeration, ancient food preservation techniques, one being fermentation, were utilized to make fruits and vegetables safe for consumption available long after the growing season had ended. It is still used by many cultures today to produce crunchy pickles, tangy fruit chutneys and zesty sauerkraut. Essentially, it is a metabolic process that converts the starches and sugars found naturally in vegetables and fruits into food preserving alcohols and gases, which hinders the growth of harmful bacteria while encouraging the reproduction of beneficial

Before the grape placed in the tap water was put in the tap water, the grape was very ovate, had a small dent on the side, and was soft on one end. After the grape was placed in the tap water and taken out, the grape was still ovate, but a little more plump. The grape also was still soft on one end, however the grape no longer had a dent on the side. When, osmosis occurred in the grape placed in the tap water, the water passing through the skin of the grape, acting similarly to the cell membrane of a cell, plumped the grape, pushing up the sunken skin causing the dent, therefore causing there to no longer be a dent in the grape. All four of the solutions in the experiment were hypotonic. The solutions’ hypotonicity was supported by the data showing how all four of the grapes in the different solutions gained mass because some of the water in the solutions diffused into the grapes. None of the four solutions were shown to be hypertonic by the data because none of the grapes lost mass throughout the

Variables that can affect the rate of fermentation are type of sugar, type of yeast, amount of sugar, duration and temperature of fermentation. In this EEI, the amount of sugar will be investigated and its effect on the rate of fermentation in ginger beer. The amount of sugar effects fermentation

Introduction: Fermentation a metabolic process with occurs in the absence of oxygen molecules also known as an anabolic reaction. It is a process of glycolysis in which sugar molecules are used to create ATP. Fermentation has many forms the two most known examples are lactic acid and alcoholic fermentation (Cressy).

Abstract – Yogurt is a product of lactic acid and lactose. In order to produce yogurt, milk is pasteurized (at 45 degrees Celsius) and the inoculum is added followed by incubation. Over a period of 7 days, yoghurt fermentation was attempted using lactobacillus cultures. A liter of milk was supplied and plain yoghurt was used as a starter culture as it contains the necessary bacteria to ferment lactose and produce lactic acid. The milk was added to a flask: then boiled, cooled and inoculated. The milk was incubated for a week and all the while the milk was tested for changes in pH, density, mass and physical changes. The values obtained were then used to determine the growth kinetics of Lactobacillus bulgaricus.