Finding The Minimal Inhibitory Concentration

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Finding the Minimal Inhibitory Concentration:
The Process and the Importance
Melissa Bañaga
San José State University

Abstract The purpose of this project was to determine the minimal inhibitory concentration of our given antibiotic called tetracycline. Tetracycline is a bacteriostatic agent and instead of directly killing the bacteria, it prevents the growth mainly by inhibiting the synthesis of proteins. We hypothesized that the minimal inhibitory concentration for our test would range from 4 g/mL to 8 g/mL. This process spanned across four lab meetings located in a biology lab at Duncan Hall of San José State University. The first lab introduced us to sterile and plating techniques as we prepared and plated a serial
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There are many antibiotics out there but it is not enough just to administer as much of an antibiotic that you want. There are particular numbers needed to achieve the desired and more longer lasting result.
Disease and plague had once completely and disastrously overwhelmed humanity for countless years. During the era when medicine was not as advanced and sickness and disease had run rampant, people had resorted to dangerous, unethical and unsanitary methods of healing. Desperate to alleviate the pain, doctors and healers of the time did what they could to make the pain and discomfort of the ill disappear. Though the intentions of many doctors was to heal the sickly, they did not realize there was a more scientific approach of solving many of their problems. On a microscopic level, there are bacteria and microbes that are attacking the body, causing diseases to ensue. In order to deal with the issue at hand, scientists had to figure out what could be used to fight these particular pathogens and not just what, but how much of these antimicrobials should be used. To better understand this idea of knowing the proper dosage needed for an antibiotic, we exercised this concept of minimal inhibitory concentration. The minimal inhibitory concentration is the lowest amount of the antimicrobial concentration needed to prevent the noticeable growth of the microorganism after incubating the sample overnight. Each group was given a specific antibiotic of
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