Finding The Minimal Inhibitory Concentration

According to data, the natural antibiotics worked better for defending against Bacillus cereus. The Oregano Oil and the Garlic had tied for first and proved to have the largest zone with a length of 18 millimeters between the disc and the bacteria after 48 hours of growth. For a bacteria such as “Food Poisoning” the final result was shocking that the least amount of growth was for the man made antibiotics because they were specifically made to fight these types of Bacteria. The final result for Gentamicin had proved it to have a much smaller zone of inhibition with a zone of just 12 milimeters after the 48 hour time period. The growth of the penicillin was the lowest of them all with the very small zone of 0 millimeters after the 48 hours.

In the last decade, the number of prescriptions for antibiotics has increases. Even though, antibiotics are helpful, an excess amount of antibiotics can be dangerous. Quite often antibiotics are wrongly prescribed to cure viruses when they are meant to target bacteria. Antibiotics are a type of medicine that is prone to kill microorganisms, or bacteria. By examining the PBS documentary Hunting the Nightmare Bacteria and the article “U.S. government taps GlaxoSmithKline for New Antibiotics” by Ben Hirschler as well as a few other articles can help depict the problem that is of doctors prescribing antibiotics wrongly or excessively, which can led to becoming harmful to the body.

Modern-day advances have allowed scientists to develop methods to control pathogens. The more common methods include the use of antiseptics, antibiotics, and vaccines. By definition, antiseptics are chemicals used to kill pathogens. A few widespread antiseptics include soap, vinegar, and rubbing alcohol. Antiseptics are primarily used for the external destruction of pathogens, while antibiotics and vaccines target internal pathogens. Nevertheless, there are and can be difficulties concerning the use of antibiotics. “As antibiotic use has become more common, antibiotic-resistant bacteria have evolved.” This resistance due to evolution is called antibiotic resistance. It results in the antibiotics having no effect whatsoever on the bacteria, rendering the antibiotics useless. When antibiotic resistance occurs, scientists must resume their search to create a new medicine to can kill the mutant bacteria.

Antibiotics:- antibiotics are medication which destroy or slow down the growth of bacteria, they are used to treat infections which are caused by bacteria. Most of the time the body’s immune system can fight off bacteria but in cases in which the body cannot antibiotics are used to destroy them. Antibiotics are either given orally, applied to the skin in ointment form or injected, this all depends on the type of infection the body is currently trying to fight off, for example skin infections are treated with ointment, oral antibiotics are used to fight of moderate infections and injective antibiotics are most commonly used in

2. Which antibiotic was most effective in killing the bacterial lawn on each plate? Which was the least effective?

Antibiotics, composed of microorganisms such as streptomycin and penicillin, kill other infectious microorganisms in the human body. At one point, antibiotics were considered to have “basically wiped out infection in the United States”, but due to their overuse and evolutionary

Antibiotics are inarguably one of the greatest advances in medical science of the past century. Although the first natural antibiotic Penicillin was not discovered until 1928 by Scottish biologist Alexander Flemming, evidence exists that certain plant and mold growths were used to treat infections in ancient Egypt, ancient India, and classical Greece (Forrest, 1982). In our modern world with the advent of synthetic chemistry synthetic antibiotics like Erithromycin and its derivative Azithromycin have been developed. Antibiotics have many uses including the treatment of bacterial and protozoan infection, in surgical operations and prophylactically to prevent the development of an infection. Through these applications, antibiotics have saved countless lives across the world and radically altered the field of medicine. Though a wonderful and potentially lifesaving tool, antibiotic use is not without its disadvantages. Mankind has perhaps been too lax in regulation and too liberal in application of antibiotics and growing antibiotic resistance is the price we must now pay. A recent study showed that perhaps 70% of bacterial infections acquired during hospital visits in the United States are resistant to at least one class of antibiotic (Leeb, 2004). Bacteria are not helpless and their genetic capabilities have allowed them to take advantage of society’s overuse of antibiotics, allowing them to develop

Section 3 of the Promise for Antibiotics and Therapeutics for Health Act or the PATH Act, which call for current PATH Act legislation to be modified so that it “will allow health experts to more easily develop new treatments for antibiotic resistant bacteria, and make real progress in presenting a great number of illnesses and deaths in the United States”1. In addition, this new legislation will impact Section 506 of the Federal Food, Drug, and Cosmetic Act (21 U.S.C 356) by introducing into its current language a new subsection (g) “Limited Population Pathway for Antibacterial Drugs”. Thus creating new avenues for the introduction of alternative treatments for limited populations based on the recommendation of Secretary

The aim of this investigation was to find out which antiseptics were most effective at preventing the growth of bacteria.

Antibiotic resistant infections are not only increasing, but intensifying exponentially. Even worse, there are few drugs readily available to diminish the swelling number of contagions; a sparse and meager quantity of combatants to tackle the vehement and aggressive pathogenic bacterium. These microscopic organisms, though infinitesimal, cause gigantic problems and wreak extra havoc when they attack the drugs prescribed to destroy and eradicate them, and survive. In culmination, they are more resilient, more powerful than ever before. Many of these superbugs are stronger and more alive, violently destroying the immune system of the body that has become their habitat, instead of ultimately dying. The tiny,

E.coli outbreaks have steadily grown over the last few decades. An expansion in big farming has led to E. coli not only being found in meat, but vegetation as well, due to waste runoff. This has increased our need for adequate antibiotics that can fight bacteria, like E. coli. The best way to pinpoint which antibiotics work is by measuring their ability to create antimicrobial agents or zones of inhibition. When a paper disc that has been saturated in an antibiotic is inserted in a solution of E.coli and medium, the zone of inhibition will be noted as the clear ring that forms around the disk. The antibiotics efficacy is then determined by measuring each disk zone of inhibition, and comparing these measurements to the zone measurements of an untreated specimen. If an antibiotic is to be deemed sufficient for treating E. coli it should show a zone of inhibition that is at least double the size of the untreated specimen.

5) My hypothesis: “The higher the concentration of antiseptic, the more powerful it will be at inhibiting/ killing microorganisms which cause bacterial growth.” This theory supports my result because as you can see the zone of inhibition increases due to the increasing concentration of antiseptic. The higher concentration of antiseptic means it has been less diluted, which means its more stronger than other more diluted concentrations, this high concentration will determine how powerful the antiseptic will be at killing/inhibiting the growth of bacterium, this high concentration of antiseptic will break down the bacterial cell wall, after this process has happened the antiseptic can stop the mutation of the bacteria and inhibit the

Although a vaccine does exist, its cost and multiple doses needed to achieve immunity have limited its acceptability by the medical community [20, 21]. Antimicrobial therapy has been at the forefront of research in trying to identify if prophylactic treatment is necessary, what drug is most successful, and what dosing is most appropriate.

The main objective of this experiment is to investigate the effect of different types of antibiotics on bacteria Bacillus subtilis and Escherichia coli. Some of the main methods used in this experiment

The overuse of antibiotics has been a problem for well over a decade. This misuse leads to many nonvisible problems arising within the human population. As the use of antibiotics increases, the number of antibiotic resistant bacteria also increases. When bacteria become resistant to an antibiotic, another antibiotic must be used to try and kill it and the cycle becomes vicious. Michael Martin, Sapna Thottathil, and Thomas Newman stated that antimicrobial resistance is, “an increasingly serious threat to global public health that requires action across all government sectors and society” (2409).