Essay on Firefly Lab Report

This lab was to investigate fermentation, a cellular process that transfers the energy in glucose bonds to ATP. The energy in ATP can then be used to perform cellular work.

The petri dish with the highest hatching viability was the 1.0% dish. No, the results did not support my prediction in the pre laboratory question #2. My prediction did not match the results because in the 1.5% petri dish it showed a lower hatching viability compared to the 1.0% petri dish. After, the results came out it was proven that the best NaCl solution for the brine shrimp was the 1.0 petri dish because it wasn't too concentrated and it wasnt too little.

Purpose: The purpose of the lab was to determine the characteristic colors produced by certain metallic ions when they were vaporized in a flame and identify the unknown metallic ions by process of

In the experiment, Luminol Synthesis and Chemiluminescence, the purpose is to create luminol using the base-mediated reduction of 5-nitro-2,3,-dihydrophthalazine-1,4-dione and to investigate the chemiluminescence reaction that luminol is known for. 5-nitro-2,3-dihydrophthalazine-1,4-dione was reduced using sodium hydrosulfite in a solution of sodium hydroxide under reflux conditions. Acetic acid was used to precipitate the solid luminol product, later the product was collected through vacuum filtration. The luminol product emitted a blue light when a dilute solution of it and sodium hydroxide was mixed with a dilute hydrogen peroxide and potassium ferricyanide solution. The blue light seen correlates to the excited molecules that are dropping

The characteristics of ATP make it exceptionally useful as the basic energy source of all cells.

An error that occurred during within this lab experiment was not weighing the beaker at the same, tampering with the results. There was chaos within the first beaker it could not be be put on the plastic balance. Making a quick decision that was not in the procedure, which was wait 3 and half mins before weighing the becker instead of 1 min after boiling out water and the cardondixode gas out. this caused the lab experiment to had a later start time. The time of weighing the sodium acetate was effect within this error caused the results to be different.

Table 4 The Concentration of The Cell Lysate & Cell Pellet In E.coli Induction Experiment

A flame test is conducted by dipping a wire loop into a solution that contains sodium or potassium ions, and then, once saturated, the loop is heated by a fire. This dissociates the salts into neutral atoms which, when heated to an ever higher temperature, excites the electrons such that they fulfill a higher energy level. When the electrons fall back down to their ground state, the resulting light will be a specific color depending on the ions present. When sodium is present the flame will be an intense yellow color, while the flame will appear violet in color if the solution contained potassium. When both are present together, however, the vibrancy of the sodium overpowers the faint color that the potassium ions emit, such that a filter made of cobalt glass must be used to block out the light of the sodium ions, so that the light produced by the potassium ions may be seen. The first week the experiment was conducted, the flame test was done on the sea water solutions, while the dehydrated salts, obtained by placing a sample of the sea water into an oven and allowed to evaporate, were used during the second

This is an osmosis and cellular transport lab. This lab is done to show us how different types of solvents can affect the growth of a cell. In this lab we are trying to solve what would happen if a cell underwent osmosis and diffusion. In this lab you could gain skills a variety of skills, for proper lab technique all the way to actually learning how osmosis and diffusion affect a cell.

In this lab a cotton swab was fluffed up by scraping it against the serrated edge of a tape dispenser and then dipped into a liquid chemical solution. Once dipped the cotton swab was placed horizontally above the fire making sure to only burn the cotton part so as to not accidently get a different flame because of the burning stick. The color of the flame is viewed through a spectroscope and then both that spectrum and the color to the naked eye are recorded on a data table. This step is repeated for the remaining four solutions and then the bunsen burner is turned off and the area is cleaned. The purpose of this lab was to observe the relationship between various elements and emission spectroscopy and identify an unknown substance. By burning

The movement of materials through a dialysis membrane was conducted in this experiment. In this experiment, dialysis tubing was used to model a semi-permeable membrane. Glucose and starch solutions were placed into the dialysis tubing, which was placed into the beaker that contained water and 35 drops of iodine (initially only 20 but 15 were added). The solution in the beaker tested negative in the glucose test. The initial color of the glucose and starch in the tubing was cloudy and translucent, and the initial color of the liquid in the beaker was a translucent yellow orange. After a few minutes of sitting in the beaker, the liquid in the dialysis tubing had become a purple color and the liquid in the beaker became a darker yellow. Iodine

When ending experiment 1, the membrane was removed from the beaker with iodine solution, and a small portion of what was inside the beaker was poured into a test tube. In this experiment, the aim was to test for the presence of glucose and prove that glucose molecules also underwent diffusion, moving from a hypertonic solution (inside the membrane) to a hypotonic solution (outside the membrane). After pouring the liquid from the beaker into the test tube, drops of benedict solution were dropped into the test tube. The benedict solution, when in contact with glucose, turns from a bright blue to orange, however, for that to happen, the benedict solution requires heat to kick start the reaction, thus, the test tube was placed into another beaker with hot water, which was previously on a boiler. After waiting for some minutes, it was observed that the blue solution inside the test tube turned orange, evidencing the presence of glucose in the solution. This proves that, besides the movement, through diffusion, of the iodine solution towards the inside of the membrane, glucose cells were also small enough to, through diffusion, move towards the solution in the

The firefly also known as, photinus pyralis, is a distinctive and unique specie as it makes itself glow, mainly to attract a mate. Bioluminescence is a fascinating process by which organisms convert chemical energy to cold light. Inside the firefly’s lantern are the chemicals – luciferin and luciferase. When luciferin and luciferase are mixed together in the presence of oxygen and ATP, the fuel for the cell, the chemical reaction gives off energy in the form of light. Bioluminescence chemical reactions use natural resources that are easily replenished and are renewable – so it doesn’t ware off or lose its glow.

Introduction Before starting his experiment, it is important to know some background information on the topic at hand, photosynthesis. Photosynthesis is when autotrophs convert light energy into chemical energy that is later released to fuel other organisms (Vidyasagar, 2015). Light energy is the energy from the sun. This is converted into chemical energy that is stored in the bonds of glucose through the light-dependent and light-independent reactions. The light-dependent reactions use sunlight and water to produce NADPH and ATP, as well as O2 as a waste product.

Living cells should be cultured on petri dishes and then incubated with a tracer in order for downstream flux analysis. Once the metabolic processes have been quenched, the next step is to lyse the cells, separating both the polar and non-polar metabolites from the other cellular substances at the same time. After these steps, the cells are shaken to completely lyse the membranes allowing for a more efficient extraction of all possible biomolecules. After shaking, there should be a clear separation between the polar and non-polar phase for the metabolites, with a well-defined interphase containing proteins and nucleic acids. After centrifugation, the different phases are now separated, and each one can be sampled for further analysis