Fluorescent Dyes Lab Report

The purpose of this lab investigation is to make 1 L of Pirate Purple dye solution. The claim states that the use of the concentration and absorbance of the Blue 1 dye and Red 3 dye will lead to the concentrations of the red and blue dye to yield 1 L of Pirate Purple dye. In order to understand molarity or concentration, familiarity with the terms of solute, solvent, and solution are very important. A Solute is a substance that is being dissolved by a solvent. A solvent is a substance that is dissolving the solute. Solution is a solute of a homogenous mixture that is dissolved in a solvent. With that being said, molarity is the moles of a solute divided by the liters of a solution (mol/L). In accordance to concentration, absorbance is used

The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in

The most polar molecules on a chromatography strip move the least, and the least polar molecules move the most. Out of red dye #40, blue dye #1 and yellow dye #5, the molecules in blue dye #1 have the polarity closest to both isopropyl alcohol, and sodium chloride solutions. Red dye #40 has the most polar molecules, while blue dye #1 has the least polar molecules.

Green fluorescent protein (GFP) comes from the jellyfish Aequorea Victoria is rare proteins with high fluoresce and absorbance. The purpose this experiments is to purify and express a His2-tagged recombinant from of GFP (rGFP) from the E. coli strain BL21(DE3)< pRSETA-GFPUV > through a series of experiments by using Ni+2 agarose affinity chromatography technology. The GFPuv gene (UV-optimized GFP) was over expressed in the E. Coil strain BL21 (DE3) (pLysS) as an n-terminal His6/Xpress epitope tagged bind protein. Then using Ni2+ Agarose affinity chromatography to obtain purification of the crude extract. Then observe under the long wavelength UV light, the activity of the rGFP in the column fraction. Bradford assay was performed to obtain the total protein amount. When calculating the

Every cell transports materials in and out throught something called a membrane. There are many different methods of transport in the cell Saccharomyces cerevisiae (Serrano, 1977) We want to know does adding higher concentrations of azide more effectively block dye transport? We tested the transport of dye in yeast cells with a metabolic inhibitor. When we did this we showed no difference in the absorbance between different azide solutions, and our control. From this we concluded that azide has no effect on the transport through a yeast cell membrane.

The Uptake of Neutral red dye by saccharomyces cerevisiae in the presence of a metabolic inhibitor

This experiment was design to find the amount of copper in a penny using a visible spectroscopy. We achieved this by combining .89 to .93 of Cu(NO3)2*2.5H2O to a 25mL volumetric flask and filling the flask to about half full with deionized water, swirl, then fill to the line with more deionized water. After, fill a 25mL burret with Cu2+ stock, with this add .5, 1, 1.5, and 2 mL of the stock to four different volumetric flask, add 2mL of 15 M NH3 from the burret to each flask and swirl, then fill to the line with deionized water. First, calibrate the spectrophotometer then; enter samples of each of the four samples in the spectrophotometer to test the absorbency. For the penny stock solution, use a 500 mL filter flask with about 250 mL of 1 M NaOH, place a rubber stopper to the top and connect a inverted long stem funnel to it using tygon tubing, and place a 125 mL flask below it. Turn on the water aspirator, place the penny in the 125 mL flask, measure out 20-25 mL of 8 M nitric acid in a 50 mL graduated cylinder, and then slowly add it. It should take about 5-10 minutes for the penny to dissolve.

It was found out that our graphs for yellow 5 and yellow 6 were half the concentrations needed to mimic orang PowerAde. This was found out with the help of the TA. Both the Full Throttle and orange PowerAde used twice as much food dye concentration then what was provided for the experiment. In order to compare our made solution with the beverage, orange PowerAde needs to be diluted to a solution that contained 50% distilled water and 50% PowerAe To do this, take 5 mL of orange PowerAde and add 5 mL of distilled water. A new absorption graph can be formed and used to compare with the made

Chemiluminescence is a reaction well known to those who enjoy crime scene shows. Luminol is first synthesized in the experiment. Then, the crude luminol produced is oxidized to release photons giving off a light blue glow in a dark room. In a crime scene show, the blood acts as a catalyst aiding in the oxidation of luminol and hydrogen peroxide resulting in the blue glow 1.

Introduction: Transformation is used to introduce a gene coding for a foreign protein into bacteria. Hydrophobic Interaction Chromatography (HIC) is used to purify the foreign protein. Protein gel electrophoresis is used to check and analyze the pure protein. Research scientists use Green Fluorescent Protein (GFP) as a master or tag to learn about the biology of individual cells and multicultural organisms. This lab introduces a rapid method to purify recombinant GFP using HIC. Once the protein is purified, it may be analyzed using polysaccharide gel electrophoresis (PAGE).

To fully comprehend the mechanism of the bleaching process, it is essential to know the structure of the blue food dye. For this experiment, the FD&C number of the blue food dye used is Blue 1, its structure is shown as below: It could be seen that the structure has a conjugated system, which is a system of connected p-orbitals. This means that the single and double bonds alternate throughout the entire system. No electron absorbs more energy than the other because the π-orbitals overlap effectively and all π-electrons in the system are distributed above and below the molecule. The electrons are delocalized in the line of the bonds, which enables the electrons to jump between energy levels.

A colourmeteric assay was carried out to enquire into the extent of hydrolysis of substrates alone and in the presence of inhibitors. The aim of the experiment was to determine the substrate specificity of the enzymes AChE and BChE, in addition to their sensitivity to several cholinesterase inhibitors.

The goal of this experiment was to use different dyes to determine the Rf value. Chromatography is done to determine the presence of a mixture in a substance. In this experiment different dyes were used such as McCormick food coloring, M&amp;M’s, Kool-Aid, and the dyes that came with the lab kit.

Laser-induced forward transfer (LIFT) based bioprinting Less clogging than other methods Creates high resolution projects Does not affect cell viability (Skardol & Atala, 2015) Biomaterials (Hardware) Vast range of materials Constantly evolving Cell supportive soft hydrogels Stiff metal or ceramic implants Synthetic or natural Complex functioning medical devices (Skardol & Atala, 2015) Computer Aided Design (CAD) & ITK-SNAP (Software)

Lasers are very important tools in modern science, industry, and everyday life. From their creation over forty years ago their designs have improved and their usefulness increased. They work on physical principles that are a mix of both classical and quantum mechanical, thus making them great examples for demonstrating the properties of light and excited states of atoms and molecules. The process of producing laser light can be complicated, but the explanation of laser action, becomes easily accessible when an explanation of the underlying physical principles, and the apparatus involved in creating it is given. Indeed, there are a number of different types of lasers, solid-state lasers, gas