Food Dyes Lab Report

The concentrations and absorbances of the red and blue dyes were used to find the concentration of the purple dyes. From the graph of the blue dye, the linear equation for absorbance was y = mx + b. From that formula came the equation y = 7.915 x 104 (x) + 0.02489, where y represents absorbance, m is slope, x is concentration/molarity, and b is the constant/y-intercept. The same set up was performed for the red dye, but the equation produced was y = 1.045 x 104 (x) +.001298. The equations found when graphing absorbance vs. concentration were used to find the concentration of the purple dyes. The absorbance for purple dye 3 on the red wavelength of 470 nm equaled 0.149 and 0.818 for the blue wavelength of 635 nm. For purple dye 1

Introduction: The goal or purpose of this experiment was to determine the concentration of Allura Red in red commercially available beverage- Gatorade. Colorimeter are used to shine a LED light through the solution and hit a photocell: it will detect an absorbance or a percent transmittance value. These “value” can be charted and examined as a calibration curve. Calibration curve is a method for determining a substance concentration in an unknown sample

For this experiment, the amounts of Red 40 and Blue 1 were quantified in six different Kool-Aid samples through the use of a spectrophotometer. This was completing by performing serial dilutions on both dyes, Red 40 and Blue 1, and then creating calibration curves for each of the six samples. The absorbance and maximum wavelength values were obtained from the spectrophotometer for each individual drink sample. Beer’s Law was used to discover the concentration of

From this graph and chart we can see that the higher the concentration the higher the absorbance, all the different concentrations were tested at the same wavelength (625nm). Also we can determine our unknown substances concentration by using the absorbance we got for it. The red dot on the graph followed by the line towards the horizontal axis indicates that the concentration of fast green was 34% or 5.1x10-3.

The values of color absorbance are effective because color absorbance has a linear relationship with concentration values, which in turn, allows us to easily find concentration values for many solutions. Beer’s law describes this phenomenon since the absorbance is directly proportional to concentration. We observed that as the color absorbance increased, the concentration of the FeSCN2+ complex ion increased. This is because as the FeSCN2+ concentration increases, the blood-red color becomes darker due to more presence of the blood-red FeSCN2+ ion. Therefore, the color absorbance increases because there is more blue color absorbed by the darker red color. We then graphed the absorbance and concentration values and created a line of best fit. Using the line of best fit, we were able to predict the equilibrium concentrations of the FeSCN2+ solutions and find the change required to reach equilibrium. Since we already knew the initial concentration of FeSCN2+ and since we already found the equilibrium concentration of FeSCN2+, we can calculate the change in equilibrium. Using this data, we were able to calculate the equilibrium concentration of all of the species in this lab, since we already knew the change from the initial concentration to the equilibrium change. Q is less than K because there was no initial concentration of FeSCN2+, but after the system reached

The electromagnetic spectrum is the range of wavelengths over which electromagnetic radiation extends (Merriam-Webster Dictionary). The visible part of the spectrum is light and we can see colors from blue to red. On the left side of the spectrum is blue where the wavelength is shorter. On the right side of the spectrum is red where the wavelength is much longer than the blue end. These wavelengths are called the visible spectrum and an example of this is a rainbow. For a light wave to be absorbed by an object, the single frequency light wave must come in contact with the object. Although light colors reflect part of the visible light, black absorbs all energy and wavelengths.

The purpose of this experiment was to synthesize two organic dyes then observe their effective dye strength on various synthetic and natural materials. This was achieved by means of chemical reactions outlined below, heat to increase the reaction rate, ice baths to precipitate out the product, vacuum filtration to isolate the product, washing with water and 95% ethanol to purify the product, and dying of the clothe to observe dye effectiveness. The test of the effectiveness of the dye required that the fabric absorb one wavelength and then reflect the opposing color, and then be able to keep the color through basic washing. The presence of the dye on the fabric can be caused by ionic interaction, hydrogen bonding, covalent bonding, or simply

To fully comprehend the mechanism of the bleaching process, it is essential to know the structure of the blue food dye. For this experiment, the FD&C number of the blue food dye used is Blue 1, its structure is shown as below: It could be seen that the structure has a conjugated system, which is a system of connected p-orbitals. This means that the single and double bonds alternate throughout the entire system. No electron absorbs more energy than the other because the π-orbitals overlap effectively and all π-electrons in the system are distributed above and below the molecule. The electrons are delocalized in the line of the bonds, which enables the electrons to jump between energy levels.

The Beer-Lambert law is also able to provide a calibration plot that is used to find the concentration of a solution based on the absorbance of that same solution. With this, the purpose of the experiment is to be able to use these tools and knowledge to be able to identify concentration, transmittance, and absorbance of a solution as well as be able to create solutions manually and

Figure 1 illustrates the absorbance values of pH 5.00 and pH 10.00 bromothymol standard solutions that were recorded from 340-800 nm. The graph illustrates the maximum wavelengths observed at each respective standard solution. This maximum wavelength value is important because it is the wavelength with the highest sensitivity and can therefore minimize random error in the absorbance values. Table 1 illustrates the different maximum wavelength values for the two standard solutions. The acidic bromothymol standard solution had a maximum wavelength at a lower value than the basic bromothymol standard solution (Table 1). These wavelength values are therefore associated with the different colors of the standard solutions.

The color violet and red are some examples of wavelengths are violet which has short wavelength, and red has longer wavelengths. White is the most effective as it is a mixture of all the colors of the rainbow. Additionally, chlorophyll is the pigment which is key in the process of photosynthesis. Chlorophyll absorbs all the different colored wavelengths, but leaves green as the colour it reflects and as the colour we

The ending result of this experiment confirms that as five test tubes are lined up with the varying level of absorbance, different results in the level of absorbance will appear as well, this is visible in above table. Thus, this is due to the varying amount of water in the solution. The blank sample had a 0.30 in its level of absorbance.

This activity was performed over two days. In this lab I learned how to read labels better on food products. I also learned how challenging it is to keep track of everything you eat. After putting in the data on Monday I realized that my total of calories consumed were lower than they are supposed to be. My BMI number is 20.4. Having that as my BMI is why I need about 2,068 calories a day. On Monday I consumed 756 less calories then I should have. A few things I noticed about my data are; overeating carbs, under eating fiber, and under eating protein. My level of carbs 65 grams above what it should be. Both fiber and protein were under with fiber being 17 grams lower and protein being 6 grams lower. Now taking a look at my

Six reactive azo dyes namely, Reactive Violet 5, Reactive Red 2, Reactive Orange 16, Reactive Blue 4, Reactive Black 5 and Reactive Green 19A were generous gifts from Colors India Inc. Pvt. Ltd. Ahmedabad, India. All these dyes were of industrial grade and are widely used in textile industries. Reactive Violet 5 (vinyl sulfone as a reactive group) and Reactive Red 2 (triazin as a reactive group) were used as model azo dyes in this study. All required chemicals and solvents used in the study were of analytical grade and were procured from popular chemical firms (SRL and S.D. Fine Chemicals, Mumbai, India). Biochemical and physiological test kits were obtained from Hi-media, Mumbai, India. The TLC plates were purchased from Merck India limited.

Light is a form of energy and composed of different wavelengths. When the light exposed to an object, the light could be reflected at the surface of the object (specular or diffuse), and it could be absorbed or scattered within the object, or it could refract or totally transmitted through the object44, 45. The wavelengths (colors) that are reflected, refracted or transmitted are perceived by receptor cells (i.e. rods and cones) in the eye and recognized by the brain as a specific color. The wavelengths of visible light range from approximately 400 to 700 nm45, 46. The wavelengths that are transmitted, refracted or reflected create the color that is perceived and till about the translucency level of the material. If all light is transmitted, the material will appear completely transparent. If all light is absorbed, the material will appear completely opaque, and the color black is perceived. However, if some of the wavelengths of visible light are absorbed and others reflected, refracted, or transmitted, the color that is perceived corresponds to the wavelengths that are reflected, refracted, or transmitted, and the level of translucency is depend on the amount of the light that transmitted through the material9, 44-46. Moreover, the reflection of the light depends on the surface texture of the restoration. Thus, a smooth surface increases the specular reflectance, in which the angulation of light reflection is equal to the angle of the light source. This reveals more of