Fruit Fly Lab Report

Throughout this experiment a number of random and procedural errors were apparent; these errors could have affected the results of the experiment in a number of ways. One experimental error that occurred during the experiment was that some flies became stuck in the food source and died. The main cause of this was the fact that the fly vials were stood up (vertically) before the flies had fully recovered from the anaesthetic. This could be overcome in future experiments by ensuring that the vials are kept horizontal until all of the flies fully recover from the anaesthetic.

The parents are both homozygous. The homozygous dominant would represent the wild type. And the homozygous recessive would represent the other fly parent of a different strain. The F1 generation would consist of 100% Wild Type but they would all be heterozygous in carrying the recessive gene.

To set up this experiment, two twenty-five gallon aquariums, 3 petri-dishes, 200 flies, rotten bananas, and yeast were used. The bananas chosen to be an accelerant for the growth of the yeast and were frozen so they would be easier to cut. The yeast was used because the drosophila melanogaster prefer this as a food source.  The vestigial and wild type flies were sexed (to determine their sex), sorted, and counted.  An initial population size of 100 total flies was decided so that it would be easier to determine the phenotypic percentage of the total population. Fly paper was placed in one of the sets of cages to impose a method of natural selection as well as the sexual selection which is being solely tested by the other set of cages.

It would be expected that the mutant F1 flies would be heterozygous for the allele responsible for the grounded trait. If two F1 flies were mated, the percentage of flies that would be expected to be wildtype in the F2 generation would be 25% mutants given that the mutant allele (ap) is predicted to be recessive and, leaving 75% to be wildtype (ap+).

The variables in this lab were different than the average science experiment. Instead of affecting the experiment to prompt different results, we just had F1 generation plants produce offspring so that we could study their specific traits. By looking at the variables, we can determine if they fit the Mendelian ratio and see if genes are linked on a chromosome.

11. The progeny of a Drosophila female (heterozygous at three loci: y, ct, and w) crossed to a wild type male are listed below:

METHODS: In this experiment, the instructor provided us with 30 ebony individuals and 20 wild type individuals. In order to get an exact amount of each type, we anesthetized the flies and counted them off by gently using a fine point paint brush. Then all 50 Drosophila were put into a population cage which had a lid that had six holes for the centrifuge tubes. Two food tubes and four clean, empty tubes were added on the first day. Each food tube consisted of half a cup full of food mixed with 6-7 milliliters of water. This was the fly medium. The food should turn blue once the water is added. Each tube was labeled with a number and with the date. Every two to three days we added one more food tube until all 6 tubes contained the fly medium. After all 6 tubes were filled, the following days after we exchanged the first food tube with a new food tube. At the end of the experiment, we fed the flies with a total of 8 food tubes. Then the flies were anesthetized, again. At the end of this four week lab, the number of living ebony and wild

Introduction Drosphila melanogaster, commonly known as the fruit fly, is an excellent organism for genetics studies because it has simple food requirements, occupies little space, is robust, completes its life cycle in about 12 days at room temperature, produces large numbers of offspring, can be immobilized readily for examination and sorting, and has many types of hereditary variations that can be observed with low-power magnification. The fruit fly has a small number of chromosomes (4 pairs), which are easily located in the large salivary gland cells. As mentioned before, the fruit fly life cycle is complete in about 12 days. First, a fertilized adult female must lay the egg, which leads to the first stage of the fruit fly life cycle, the egg stage.

     There is a chance that there may be different outcomes to the F2 generation due to the possibility that the Line A and the Line B generation were not all homozygous dominant in wing type for females and eye color in males. Such a genotype in the males, vvBb, and such in females, Vvbb, may lead to a different ratio in the F2 offspring.

we said goodbye and placed them in the fly morgue. We allowed the F2 larval

The activity of an organism may be affected by different light intensities. One of the species believed to be affected by this factor is the fruit fly, Drosophila hydei (source). Based on my personal observations of the Drosophila hydei, they are approximately four millimeters in size and have a dark brown body connected to six legs and a pair of wings. A distinct and important feature of the organism in sensing light intensity are its red eyes, which are located on the sides of its head. In response, the Drosophila hydei's six thin legs are used for movement. Its pair of wings would be used in natural conditions but for our experiment we are using a mutant species which can not fly. Another feature which is not used for sensing light intensity but for hearing and smell for example, are two small antennae between its eyes.

For our first generation (F1) of flies we chose to cross apterous (+) females and white-eye (w) males. We predicted that the mutation would be sex linked recessive. So if the female was the sex with the mutation then all females would be wild type heterozygous. Heterozygous is a term used when the two genes for a trait are opposite. The males would all be white eye since they only have one X chromosome. If the males were the sex that had the mutation then all the flies would be wild type but the females would be heterozygous.

Your response was nicely written. As the fruit flies are similar to humans that they carry a dopamine receptor in their brains, genetics are able to conduct research on chemicals and neurons in the brain. To be honest, after I first watched the video, I was confused and did not understand the research was doing. How can you teach a fly to learn? Also, how were they able to insert good genes inside the fly's brain and test for hyperactivity and distinguish abnormalities. These are small creatures, they were able to gather lots of information. There need to be new medications that reduce the side effects. For individuals with co-occurring disorders should be treated with medication that will help both disorders, not only one. Just like David

The F1 generation that we had just decanted was then moved to a dissecting microscope for scoring, this generation included 20 Drosophila flies that we could then inspect for different traits using the microscope (traits being the sex of

Therefore, it is very important to develop the most suitable culture medium for the cultivation of Drosophila. The food sources of the Drosophila are mainly ripened fruit and vegetables. Drosophila more prefers to consume the yeasts that grow on and ferment the fruit. Ripened banana pulp is the most common food source for Drosophila in laboratory. In recent times, the agar-like culture medium is developed to culture the flies. There have variety of recipes and formulas to making the culture medium. The condition of the medium ought to be suitable for the larva to tunnel into to obtain the food and for the flies to move on top without sticking on it.