Fruit Fly Lab Report

In this experiment we tested to see what the offspring of an unknown cross of an F1 generation would produce. After observing the F2 generation and recording the data we found some of the Drosophila showed mutations, two in particular. The mutations were the apterus wings, and sepia eyes. After collecting our data through observation, a Chi-test was conducted resulting in a Chi-value of 5.1 and a p-value of .2. Since the p-value was greater than 0.05, there was no significant change in the data. This proved that the Drosophila flies still followed the Mendelian genetics of a 9:3:3:1 ratio.

Drosophila melanogaster is a small, common fly found near unripe and rotted fruit. It has been in use for over a century to study genetics. Thomas Hunt Morgan was the best biologist studying Drosophila early in the 1900’s. Morgan was the first to discover sex-linkage and genetic recombination, which placed the small fly in the forefront of genetic research. Scientists have used Drosophila for many reasons. For one they are very easy to maintain, breed, anesthetize, and kill with little equipment. They are also very small and it is easy to distinguish males vs females and sexually mature flies and virgins. At lastly, the flies have a very short two week life span. On days 2-7 of their life

Introduction: The intention of this lab was to gain a better understanding of Mendelian genetics and inheritance patterns of the drosophila fruit fly. This was tasked through inspecting phenotypes present in the dihybrid crosses performed on the flies. An experimental virtual fly lab assignment was also used to analyze the inheritance patterns. Specifically, the purpose of our drosophila crosses is to establish which phenotypes are dominant/recessive, if the traits are inherited through autosome or sex chromosomes and whether independent assortment or linkage is responsible for the expressed traits.

melanogaster, leaving B and D to be our mutants. Before crossing our populations, we made not of each one’s phenotype in order to see how crossing them would affect their phenotypes: Population B flies had no wings and red eyes, population D had full wings and black eyes and population G had full wings and red eyes. We expected the resulting phenotypes to be some sort of combination, revealing which traits were dominant. However, what we did not expect was the abnormal mutant that arose in a couple of our populations.

The expected number of wild type flies in the F2 generation is 734.25 and the expected number of shaven bristle flies in the F2 generation is 244.75. This, again, exhibits a 3:1 ratio of normal phenotype to affected phenotype.

11. The progeny of a Drosophila female (heterozygous at three loci: y, ct, and w) crossed to a wild type male are listed below:

Purpose The purpose of this experiment is to determine whether the fruit flies were dominant/recessive or linked/non-linked. The traits I chose for this activity was the fruit fly with vestigial wings and purple eyes, the other fruit fly I chose was a normal fly, also called wild type. While writing out my plan for this activity I thought it would be interesting to test a female mutant and the wild type male, the ratios I came up for this experiment was 2:2 and the mutant allele being recessive to the wild type. In this case, the words recessive and dominant means, if the child born from the parents inherits more of the genes and traits from let us say the father then the alleles of the father is dominant over the mother’s genes.

The first thing that was done in the Fruit Flies experiment was to prepare the food of the obtained fly food vials that were given by instructor Dr.Kang. The preparation to make the fruit flies vial with the food was to obtain a disposable plastic vial in which the fruit flies were maintained. As well as

Introduction For centuries, researchers have used Drosophila melanogaster, the common fruit fly, to study genetics. The benefits of using the fruit fly includes: its relatively short generation time, its large amount of available offspring for data, it is easy to store and handle in the laboratory and it is easily and cheaply obtained. Cross-breeding of four types of fruit flies were used in this experiment including: wild type males with normal wings vs. vestigial wing females, wild type males with red eyes vs. white eyed females, wild type male with red eyes vs. sepia eyed females, and wild type males vs. wild type females. In basic mendelian genetics, the terms dominant, recessive and sex-linked are used to describe the different types

The approximately 30 wild type males were and kept alive in order to cross mate with the females. All of the flies from all four vials that were collected were part of the P generation. Crossing the flies was the next

Introduction Gregor Mendel, the father of genetics, established the basic principles of heredity by crossing different varieties of pea plants and observing the succession of traits in the resulting generations. In order to studying the trends of heredity, model organisms are crossed and observed for the resulting traits. Drosophilia melanogaster, the fruit fly, has been a useful species in the study and practice of genetics. The fruit fly is an excellent model organism due to its short generation time, large offspring numbers, simply and cheap care, easy handling in a lab setting, and large and varied stocks available with minimum cost. It was among the first organisms to be used for genetic analysis (Pierce, 2005).

The vials were separated into males and females. Five male and female from each vial were put in a specific vial ending up with 20 flies in one vial. It was made sure the group had enough flies; any extra flies were distributed among the groups to assure everyone has the correct amount of flies for each genotype. The exact number of flies for all four phenotypes ( male (+/+), male (e,e), females (+,+), and female (e,e)) were used. Any remaining flies not need by anyone were put cake into the cultured vial where they came from.

we said goodbye and placed them in the fly morgue. We allowed the F2 larval

The purpose of our experiment was to see the progeny from the cross between the wild-type females and the mutant males and the cross between mutant females and wild-type males. We started by preparing the vials for the test cross. We had first prepare that inside the vials that will provide nutrients to the fruit flies so that they can carry out their life cycle. To do this, we obtained a clean vial and a foam plug. Next, a small scoop of powdered food was added to the vial. Then a small scoop of tap water was added. Once the mixed, approximately 4 grains of yeast were added. The vial was then tapped on the table to level out the mixture. The inside wall was then wiped with a clean Kim-wipe to keep the flies from sticking to the wall. This

This experiment looks at the relationship between genes, generations of a population and if genes are carried from one generation to another. By studying Drosophila melanogaster, starting with a parent group we crossed a variety of flies and observe the characteristics of the F1 generation. We then concluded that sex-linked genes and autosomal genes could indeed be traced through from the parent generation to the F1 generation.