Fungi Growth
Introduction
All humans have come into contact with some form of fungi at some point in their lives. Sometimes fungi produce large mushroom like structures, or sometimes they just appear as small wisps of hair. But with so many different forms and variations of fungi, sometimes they become hard to classify, and there is confusion as to whether it maybe a plant or bacteria. Buckholz et al (2016) state that the fungal kingdom deserves to be neither a prokaryote not a plant. Fungi are eukaryotic organisms that contain a membrane bound nucleus. They are also heterotrophic and consume their carbon. The main difference between animals and fungi is that fungi perform their digestion externally (Buckholz et al. 2016). This leads to the importance of fungi: decomposing. Fungi maintain crucial processes in terrestrial ecosystems as decomposers of dead plant tissues and mutualistic partners of almost all terrestrial multicellular organisms (Heilmann-Clausen et al. 2015). Without fungi,
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We dipped the forceps in isopropyl alcohol to sterilize them. Then we quickly opened the petri dishes, inserted the substrate, and reclosed them to avoid contamination from airborne particles. To keep the environment moist, we added 1 mL of water to the petri dish. We shook the tube to ensure that we received an adequate number of spores. Using a micropipette, we took 50 microliters of each species of fungi: Aspergillus niger, Rhizopus stolonifer, and Penicillium chrysogenum. Each fungus was placed onto the orange peel in the petri dish. We then sealed each of the petri dishes with parafilm, taped them together, and then placed them in an incubator at 25°C. The samples remained in there for 1 week. After the week, we took them out of the incubator, and making sure not to open them, we observed the growth of each fungus. All of the petri dishes and samples were then disposed
In this lab, the organism that we have been working with is the bacterium, Serratia marcescens. S. marcescens is a member of the Enterobacteriaceae family, and tends to grow in damp environments. S. marcescens is an ideal bacterium to work with in the lab because it reproduces quicker than other bacterium. This bacterium produces a special pigment called prodigiosin, which is red in color. The prodigiosin pigment is intensified when S. marcescens is grown at higher densities. During our experiment, temperature, pH, salinity concentration and oxygen requirements were tested on S. marcescens to measure their optimal growth and prodigiosin production.
The purpose of the study was to detect the rates of hyphal tip growth and nuclei position of N. crassa in influence of drug medium, Taxol. The results of the experiments show that the rate of hyphal tip growth and nuclei position are much lower in drug medium than in the control. Taxol have different effect on growth of tip and distance travelled by nuclei. Moreover, the rate at which the nuclei displace and growth of the tips are dependent on the medium and not on each other. Thus, the results conclude that drug effected fungus affects the tip growth rate and nuclei displacement.
From our data, the main interpretation that can be observed is that in Trial 3, where 2 mL of yeast was used, the rate of reaction was the greatest. This means that the enzyme activity is the greatest where the highest concentration of enzyme was used which supports my hypothesis. As the concentration of the enzyme used increases, the enzyme activity also increases. The trial with the least amount of enzyme concentration (only 0.5 mL yeast used) had a rate of reaction value of 0.667 mL/second while the control (1 mL of yeast used) had 0.733 mL/second and the trial with the greatest concentration of enzyme (2 mL of yeast used) had a rate of reaction value of 1.07 mL/second. This difference was significant enough to be able to conclude that increasing
In “Looking at mushrooms”, they tell how mushrooms live, function, decompose, and how they help the enviorment. In the pharagraph of “How Fungi Function” it list all the roles and states, “Some are decomposers, some form partnerships with living plants, and some are parasites.” This text cited goes on telling about the individual fungi role, function, and what the mycologist studying them has to say about it. In the other hand, Charlene Brusso and her passage only exclaims 1 fungi type and goes on about its uses, not its function and therefore she doesn’t have a clear description of the fungi and needs to talk more of its discoveries.
Fungi are multi-celled organisms that form a third Kingdom of life, along with the plant kingdom and the animal kingdom.
1. The authors from this experiment were trying to gain information on how the diversity of gut microbes in humans evolved the CAZymes, to supply the body with its energy needs, from microbial populations living outside the body. 2. It is known that sulphated polysaccharides are the origin of carbon for heterotrophic bacteria that make the CAZymes in marine organisms. Also, these enzymes have been seen throughout human evolution to locate polysaccharides in terrestrial plants that have been eaten.
The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in
Abstract: Many people use enrichment to grow cultures for mutant colonies. In this specific experiment we selected to kill the non-auxotrophic cells and find any auxotrophs that did not die during an enrichment process. We
October 17, 18, and 19, samples were collected from multiple sites along the BSR. The class was split into groups, and samples were collected from seven separate locations along the river and WWTP. There was also a sample collected by the S which is located between sites four and five. For each of these sites, there were ten groups from other labs that also collected a sample from the BSR. At site two of the river, the location included multiple sources of possible contamination. A drainage site was located 200 yards upstream, along with a small PVC drainage pipe next to the collection site. Not only was there drainage running into the river, the site was under a bridge, and contained other trash scattered throughout the area. The
Citrobacter Freundii is a species of bacteria that can be potentially harmful to humans. It is known to cause meningitis by protruding into the brain and replicating itself (1). The Citrobacter species has also been found as a cause of some urinary tract infections, diarrhea, and even gastrointestinal diseases and symptoms (3). C. Freundii can be located in a wide variety of soils and water (3). Lastly, it is also the cause of many nosocomial infections due to its presence in water (1).
An association between enzyme production, gene copy number, and gene evolution was explored by conducting analysis of the salivary amylase enzyme, AMY1A gene copy number, and the ancestral starch consumption in Homo Sapiens (Tracey 2017, p.22). It was hypothesized that the relative amount of starch consumption was very high for my personal ancestral diet, thus my AMY1 diploid gene copy number in my genome and salivary amylase concentration would be significantly higher than the population mean. With a population of 28 subjects (n=28), individual saliva samples were collected and compared to a calibration curve to determine the approximate amylase concentration by analyzing absorbance values. Individual samples of buccal cheek cells were
Abstract: This lab’s purpose was to see how different levels of yeast, distilled water, and sugar interact to affect the level of carbon dioxide evolved in fermentation. In this experiment we had two sections. The first section tested four test tubes with varying levels of yeast, glucose and distilled water for evolved carbon dioxide levels. The tubes were timed for 20 minutes. The amounts of solution in the test tubes are noted in the methods section of this lab report. The second section of the lab used three test tubes and flowed the same procedure except added spices. The levels of ingredients are also in the methods section. The main goal of this experiment was to see the effects of yeast concentration.
The sixth lab I completed in Biology 101 taught me how autotrophs (self-feeders) and heterotrophs (other-feeders) make organic food molecules by using photosynthesis. Photosynthesis uses the energy from the sun and it is captured and stored in the chemical bonds of organic molecules. The sunlight consists of different wavelengths of light. In plant chloroplasts, they have different pigments that capture different wavelengths of light. Light capturing pigments in green plants are called chlorophylls and these absorb all the colors of light except green, which is mostly reflected. To separate molecules from each other according to their solubility in a particular solvent is done by the process of chromatography. This basically means that polar
1. Lab reports are to be computer-generated and double-spaced. All sections of the report must
Bread. A common household food item used in pizza, sandwiches, burgers, and more. But when bread sits out too long you start to notice some fuzzy stuff growing on it. This fuzzy stuff is Rhizopus Stolonifer or, more commonly known as, black bread mold. This mold is a member of the of the phylum Zygomycota, which have life cycles that include a zygospore. A zygospore is a resting spore that has zygotes made when the mold is in its sexual phase of its lifecycle. Rhizopus stolonifer reproduced asexually and sexually. This happens when two hyphae come together, from different mating types, and form gametangia. The gametangia the fuses