Gel Testing Lab Report

This experiment will be done as the first step in determining an unknown protein. The second part of the protein determination will be done using the Western blot technique. We will use the Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) method to separate proteins by their molecular weight. This method is often used to determine the purity and weight of a protein. Electrophoresis is a method of separating proteins based on their chemical and physical properties. Gel electrophoresis has a gel support mediums that includes polyacrylamide, starch and agarose. The gel is chemically inert, it is easy to handle, and can me made to fit a desirable porosity. This makes it good to use for molecules with high molecular weights. After separating the gel, it will be stained with Coomassie Blue to visualize the protein.

The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in

The goal of this experiment is to determine the blood types of the samples given and to learn what interactions occurred to each blood type. Determining an individual’s blood type and how it reacts with Anti A, Anti-B, and Anti Rh serums played a crucial part in this experiment. The researcher concluded that agglutination (clumping) occurred in some of the blood samples. For example, Mr. Smith’s blood reacted with Anti-A and Anti-Rh serums (antibodies) allowing the researcher to determine the blood type is A. Mr. Jones’s blood reacted with Anti-B serum but it did not react to Anti-A or Anti Rh allowing the researcher to believe that the blood type is B. Mr. Green’s blood reacted with all serums and caused a reaction to occur resulting the blood type to be AB positive. Mr. Green’s blood also had a positive marker for Rh factor. However, Ms. Brown’s blood had no reaction at all and the researcher determined if no reaction occurred then the sample had no antigens but proved to have some antibodies, resulting in blood type to be O. The purpose of this experiment is to determine whose blood has type A, B, AB, or O.

To explain the ABO and Rh blood groups and to identify the universal donor and the universal acceptor (recipient).

Blood typing is a way to identify and match blood samples. Three separate tests are performed. The patient 's blood is mixed with antibodies that bind to the A protein. If the patient 's blood clumps or agglutinates, that means that the person 's blood contains protein A. If the blood being tested does not react with the antibodies that bind the A protein, then that person 's blood does not contain protein A. Similar testing

2. When 2.00 g of NaOH were dissolved in 49.0 g water in a calorimeter at 24.0 ˚C, the temperature of the

A blood transfusion is the act of giving red blood cells to a patient through an IV.

October 17, 18, and 19, samples were collected from multiple sites along the BSR. The class was split into groups, and samples were collected from seven separate locations along the river and WWTP. There was also a sample collected by the S which is located between sites four and five. For each of these sites, there were ten groups from other labs that also collected a sample from the BSR. At site two of the river, the location included multiple sources of possible contamination. A drainage site was located 200 yards upstream, along with a small PVC drainage pipe next to the collection site. Not only was there drainage running into the river, the site was under a bridge, and contained other trash scattered throughout the area. The

1.Full blood counts: To assess the Hb for evidence of anemia, WBC for evidence of infection.

Purpose: is to determine the unknown bacteria with a variety of biochemical tests. There are many reasons that contribute to why it is so important to test patients for both high and low risks diseases. The most important reason would be to know the identity of microorganism and how it can be treated .This study was performed in microbiology laboratory class by applying the microorganism to the tests that have been performed in the class prior to the identification of the unknown. First, the lab professor handed out a bacteria that was on the unknown streak plate labeled B5 that consisted of an unknown gram positive or gram negative bacteria.

You can view macromolecules by using a technique called gel electrophoresis. Gel electrophoresis is a process used by scientists to study and view macromolecules. This process separates the different macromolecules and coats the different macromolecules so you can easily see them. A macromolecule is a very large molecule that contains many atoms. Agarose is used in gel electrophoresis to coat the macromolecules.

To determine the blood type of each member, we placed two drops of the same blood into a single tray and used a new tray with new blood for each member. Once placing anti-a serum on Mr. Thomas’s blood sample, we mixed

The purpose of this lab is to correctly determine the blood type of four unknown synthetic blood samples through the use of three antibody serums; anti-A, anti-B and anti-Rh. By successfully determining the blood type of an individual, information such as the individual 's blood antigens and antibodies will be known and can help determine which blood types that individual can accept or donate.

5 / 10 To prep our purified PCR product and our PCR control for electrophoresis, we added 4 microliters of Blue Juice loading buffer to each sample. The samples were then loaded into the wells of the gel along with a DNA Size Standard. The electrophoresis chamber was then turned on and the voltage setting was switched to high. The electrical current applied to the chamber caused negatively charged DNA molecules to move from the positive end to the negative end, with the smallest fragments moving the furthest.

However, IIF is a subjective method in discovering immune diseases, it depends too seriously on the experience of physicians and unfortunately the accuracy of inter-laboratories agreement drops to 76% for recognition of stained patterns which require more detailed diagnosis [7]. This drops is due to many reasons like the Quantitative information which supplied to physicians are not enough, reading from systems and optics are varying from each other, irradiation occurred through the photo-bleaching effect caused by the light [8] and the reproducibility of the diagnostic protocol is low [9].