Mammalian Gene Digestion Lab Report

As health care has advanced through the years, many roles have changed which includes those of risk management and patient safety. Once thought to be one in the same,but they have distinct and obvious differences that set each apart from the other. Risk management is defined very broadly by the United States Inspector General of the Department of Health and Human Services as "any activities,process, or policy to reduce liability exposure"(https://oig.hhs.gov/oei/reports/oei-01-03-00050.pdf).This is much different from the definition of patient safety as found in the book Advances in Patient Safety: New Directions and Alternative Approaches as "Patient safety is a discipline in the health care sector that applies safety science methods

As a health care professional, I will help to ensure that work environment is safe by following the rules. Trashing materials in appropriate cans is the most important for all employees in the building. Knowing my responsibilities for my own health and safety in the workplace. If I found something unsafe condition, I will report immediately to my employer. Using personal protection equipment is important for your own safety.

This essay is about why lab procedures and practice must be communicated in a lab.

Risk assessments are vital to be included within a laboratory setting as it involves evaluating the potential risks which may be involved when completing an experiment or activity. The employer must be aware that risks which someone is exposed to at work must be reduced by them responsibly implementing precautions in order to make the setting safer. They need to analyse: what could possible go wrong? How likely is it? And what would be the consequences? These questions are regularly used when creating a risk assessment so that every risk sheet has a similar outline and meets the criteria of an effective risk assessment. It covers the general procedures, the working environment and the handling, storage and transportation of various substances.

10. Which had the greatest average 1- OD (amount of bile acid adsorbed to meal particles), the standard meal with bile acid or the

The Pennsylvania Patient Safety Authority is a state agency founded by the Medical Care Availability and Reduction of Error (MCARE) on 2002. Moreover, the agency creates the greatest database system for patient safety which known as Pennsylvania Patient Safety Reporting System PA-PSRS. The system was developed by contract with Pennsylvania-based independent, ECRI, in partnership with Hewlett Packard Enterprise, a non-profit health services research agency, the Institute for Safe Medication Practices (ISMP), a Pennsylvania-based, non-profit health research organization and also a leading international information technology firm. Statewide compulsory for using PA-PSRS to report serious events in hospital, ambulatory surgical facilities and

There are many hazards in different scientific environments, in this assessment the Jack Hunt School Science Laboratory, the Peterborough Power Station and the Jack Hunt School Kitchens will be the different scientific environments observed. The hazards in each environment, the safety legislation that is put in place to prevent danger as well as other safety measures that are taken by those who work in these environments will be explained.

It is a great opportunity to have this experience and to relate it with what we were being taught at school. There are a lot of connections in this project regarding patient safety. My safety project is a qualitative analysis of the difference between an allergy and sensitivity. The question still lies on how could the hospital staff manage allergy better?

For PEI Transfection, we added 1 ml of Trypsin to a T-25 flask of 293T cells and incubate at 37 0C for 10 minutes. Then, we supplemented 10 ml of DMEM (2.5%), FBS (15), P.S medium to the flack and finally transferred to dish and put back in CO2 incubator. After 5 days, we diluted plasmid DNA (ug) in 250 ul of 150mm NaCl in a sterile tube, and diluted 40 ul of PEI into 250 ul of 150 mm NaCl in second sterile tube. After that, incubated each separate tube for 5 minutes at RT and then mixed the DNA tube with the PEI tube. We put the tube in incubator for 2-5 minutes at RT, supplemented dropwise to cells, and put back into incubator. The important steps that was added helper virus (VSV) to cells and put it in the CO2 incubator; then, collected supernatants that contains our

All staff should know the standard safe working practices in a lab. They must know the types of hazard that they face; the worst event which could occur; and also the personal protective equipment and facilities which will reduce these risks. Safety showers and eyewash stations are included in these facilities. Lab employees must know where these are situated and how to use them. Each employee should also be familiar with all alarm signals, the evacuation procedures and the response to an emergency. If the lab worker knows all these then they should be safe while working.

Within a microbiology lab extra precaution must be taken due to the vast amount of different organisms being analysed as infections and diseases may arise therefore it is vital to consider and implement various safety precautions to prevent a breakout. Micro-organisms being examined can be classified as being a pathogen; an agent initiating disease/illness towards its host, for example an infectious particle or organism being able to produce a disease/illness in another organism. An example of a type of bacteria that is considered pathogenic would be Mycobacterium tuberculosis (causing tuberculosis). (http://www.biology-online.org/dictionary/Pathogen). There are many hazardous chemicals present in a microbiology lab as well as the equipment

Describe one experience in this course and how you will use it in care of a person in your clinical practice (Remembering, Understanding, Applying)

Plasmid map of pRSETB. Primers were designed to amplify via PCR to cDNA. The PCR product was digested with XhoI and EcoRI enzymes and ligated into the pRSETB plasmid. The pRSETB plasmid contains a T7 promoter region, is ampicillin resistant, inducible with Isopropyl β-D-1-thiogalactopyranoside IPTG, a molecular mimic of a lactose metabolite that triggers transcription of the lac operon, and has XhoI and EcoRI cut sites. The pRSETB plasmid is transformed into dH5α E. coli and plated on carbenicillin plates. Colonies are selected and grown on a carbenicillin plate while PCR is used to check that the plasmid that was up-taken was not

* Hand washing is the most important method of preventing the spread of infection by contact (Ayliffe et al 1999). The Nottingham University Trust Policy on Hand Hygiene (2009) states that there are three types of hand hygiene, the first is ‘routine hand hygiene’ which involves the use of soap and water for 15 – 20 seconds or the application of alcohol hand rub until the hand are dry. The second is ‘hand disinfection’ which should be used prior to an aseptic procedure by washing with soap and water and applying alcohol hand rub afterwards. The third is ‘surgical hand washing’ which is the application of a microbial agent to the hands and wrists for two minutes. In addition to which a sterile, disposable brush may be used for the first surgical hand wash of the day although continued use will encourage colonisation of microbes. The third example is the most appropriate to any O.D.P undertaking the surgical role as it is the best way for the surgical team to eliminate transient flora and reduce resident skin flora (World Health Organization 2010). The first and second are important to any O.D.P undertaking any other role within the Operating Department as this is the best way to reduce the transient microbial flora without necessarily affecting the resident skin flora

This lab and its procedures are all about finding out the unknown identification of a given bacteria. The lab consists of specific techniques, tests, chemicals, and vocabulary that are necessary for the finding of the bacterial identity. A bacterium is randomly assigned and it is a group effort to find the bacteria name through many of its specialties and characteristics. An example of classifying it would be to determine whether the bacteria is catalase negative or positive, or if the species is gram negative or positive. This lab is of huge significance because of its medical microbiology connections. Scientists Gurtler and Stanisich explained the connections more eloquently. They stated, in their medical article, that, “Medical microbiology