General Unknown . Introduction: . The General Unknown Lab

1482 WordsMay 7, 20176 Pages
General Unknown Introduction: The general unknown lab combines methods and ideas around biochemical and physiological activities of microorganisms for the identification of unknown pure culture samples of gram positive and negative bacteria. The purpose of using biochemical and physiological aspects of bacteria for testing and identification is used in everything from choosing an effective treatment for microbial-causing diseases to understanding the chemical reactions that occur within our own bodies (Barnini et Al., 2015). The general unknown lab’s experiments focus on biochemical and physiological characteristics of Enteric and Cocci bacteria, in aid of identification. The difference between gram-positive and gram-negative…show more content…
The unknown pure cultures 39A and 39B were presumed to be one of the listed bacteria in the previous sentence. During the first lab on April 25th, a gram stain was preformed on the samples 39A and 39B to identify which sample was gram positive (Cocci) and gram negative (Enteric), which concluded that sample B was positive and A was negative. Afterwards, both samples were inoculated onto a NUT slant for long-term storage and further biochemical testing. NUT slants are needed for long term testing, as pure culture samples in a test tube would only last a few days. In addition, Hemolytic activity and EMB biochemical tests were preformed on the samples. The first test used was the Hemolytic Activity Test, which is based the lysis and destruction of red blood cells. The test is used to distinguish between the different types of Gram-positive cocci. The results of the test can be classified as Alpha, Beta, or Gamma for the bacteria. The test is significantly helpful in distinguishing between different types of Gram-positive cocci, mainly Streptococcus, Enterococcus, and Aerococccus. The second
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