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Genetic Distance Lab Report

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Materials and Methods The Materials involved in this lab include: saline solution, micro centrifuge tubes, micropipettes, a plastic cup, an ice bath, primer mix, nuclease free water, PCR enzyme mix, 1% Agarose gel, and loading gel. Day 1 of the experiment, we started by using a permanent marker to label a 1.5-mL tube and paper cup with our assigned number. Next, we gently rinsed your mouths out with water at the sink to remove food debris. Then, poured saline solution into our mouth (5-10ml), and vigorously rinsed our cheek pockets for 30 seconds, and expelled saline solution into the paper cup. Next, we swirled the cup gently to mix the cells that may have settled to the bottom, then use a micropipette with a fresh tip to transfer 1500 …show more content…

Genetic distance is a relatively simple statistic that uses differences in allele frequency to gauge the relative distance that separates two populations in genetic space, 0 being the least distance and 1 being the greatest. I compared African American with Filipino and I predict that these populations are the complete opposite of each other, in a way that the majority of Filipino’s have the Alu insertion, and the majority of African Americans do not. I also compared African American with a Turkish population. I predicted that both populations carry + Alleles, and that the Turkish population is close to half of carrying both alleles. Finally, I compared Greek to a Taiwanese population, and my prediction is almost the same as the first comparison between African American and Filipino. I predicted that these two populations would be opposite in the alleles they carry. The Greek population would be majority homozygous recessive (no insertion), and the Taiwanese population would be majority homozygous dominant (Alu insertion). The pictures of these tests are shown …show more content…

I believe that it did not work because of the amount of DNA inserted into the wells of the gel. My hypothesis before the start of the experiment was that no one was going to have the Alu insertion. Once the class data was entered in to the database, it showed that only one person had two copies of the insertion allele, and that 3 people had one copy of the insertion allele. Later, it was found that no one in the class had both alleles of the insertion, but because the data was already in the database, the results were already affected. I know that if there error did not take place then my hypothesis would be

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