Kayla Berezne
Mrs. Cohen
Honors Biology
24 March 2013
Genetic Predictions in the Fruit Fly The Drosophila melanogaster is a fruit fly with a very short life cycle. They can be winged or wingless, and have red eyes or white eyes. The different options are called alleles. Alleles are the variants of a specific gene, and one is received from each parent on each chromosome. (“What Are Dominant and Recessive?”). It was chosen to use winged females and wingless males to predict the offspring in this experiment. The winged allele is dominant, meaning it only needs one allele to physically appear. The wingless allele is recessive, which gets covered up by the dominant allele (“Fruit Fly Genetics”). Each trait has two alleles in the flies’
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15) Bring the flies to the morgue (the bowl of isopropyl alcohol) to kill them.
16) After another week (day 14) the F₁ generation flies will hatch from their larva. Anesthetize them by repeating steps 10-14.
17) Observing the flies under a microscope, record the genders of the flies and the phenotypes that was chosen to study (the notecards and paintbrush may be helpful to do this).
18) Create a new vial with food by repeating steps 3-5.
19) Collect 5 males and 5 females of the F₁ generation and place them in the vial to become parents of the F₂ and seal it with another plug.
20) Dispose of the rest of the flies in the morgue.
21) In the next few days, continue to anesthetize and count the F₁ generation flies in the original vial.
22) After the flies have been counted, discard them in the morgue so they are not counted the next day.
23) On day 21 (three weeks from the start date) finish counting the F₁ flies.
24) Also, anesthetize and remove the adults in the second vial so they do not mate with their children.
25) On day 28, anesthetize, count, and record the phenotypes of the F₂
In 1961, a man named Dick Cabela decided to sell fishing flies that he recently purchased at a furniture show in Chicago. Once he returned to his home in Chappell, Nebraska, he created a newspaper ad for the paper, Wyoming paper that read: “12 hand tied flies for $1.” Unfortunately, Dick Cabela could round
Now mate a mutant F1 female fly with a mutant F1 male fly. Out of the 50 F2 progeny, what percentage of flies are wild type and what percentage are mutant
6. Repeat this process for each generation and make the proper adjustments required for each.
24-In a café the server developed a routine to set the table wares napkins while also taking lunch break the health inspector---
The F2 Drosophila produced from crossing the wild-type offspring of the parental cross wild-type males and no-winged females. The F2 generation consisted of 38 wild-type females and 35 wild-type males, totaling 73 Drosophila. There were also 16 no-winged females and 11 no-winged males, totaling 27 Drosophila. Therefore, the total number of Drosophila counted was 100. The phenotypic ratio was 3:1, wild-type: mutant. If the mutation were autosomal recessive the F2 generation’s hypothesized phenotypic ratio would be 3:1, wild-type: mutant. If the mutation were autosomal dominant the F2 generation’s hypothesized phenotypic ratio would be 1:3, wild-type: mutant. The expected ratio for the F2 generation was 3:1, wild-type: mutant. When chi-square
Fruit Flies were observed throughout most of the experiment. Observing the sex differences between male and female under the microscope help identify the phenotypes as well. For example, it was possible to see if the fruit flies were female or male wildtype, vestigial, white-eye and white eye vestigial. The observations to distinguish female or male fruit flies is that males fruit flies have sex combs on front of their legs. Another observation that can be distinguish between male and female fruit flies is that males have a darker ventral posterior section in the abdomen. Female fruit flies is just plain nothing showing. And lastly, another observation that is used to distinguished between male and females fruit flies is the size. It is considered
5. What possible explanations can you offer your findings? We had to put all the tools we had in use in order to complete the lab.
Question: How do the dominant or recessive genes in particular traits in a cross between a male and female Drosophila determine the traits of its offspring?
A determining factor for how rapid the life cycle of Drosophila’s occurs is the environment. The first hypothesis for this experiment was, it is expected for the parents placed in the vials to die in increased temperature after one week. Also it was expected a new generation would be present in both vials after one week with the expectation of the progeny in the early larvae stage. The final hypothesis was there won’t be too much of a difference in the vials simply because the temperature difference was not
In all of our crosses there was room for error that could be prevented in a future study. Our method design for anesthetizing the flies could be made more efficient. There were multiple times our flies were killed while in the
The way they survive is pretty cool. First they will go to the bottom of the lake and make little holes and then go back find food. The fly can find food out of the water and in the water. But if they’re not when enough for to dry they will die.
Drosophila melanogaster is a small fruit fly that feeds on fruit and the fungi growing on spoiled fruit. Fruit flies have been used in the research end of the scientific community for over a century due to their interesting physical and behavioral characteristics, their practicality and small size, and their short life cycle of about fourteen days. Its behavior has been the focus of many experiments, beginning with Thomas Hunt Morgan in 1907, and continues today in the laboratories of high school classrooms. In this experiment we are investigating the relationship between a model organism, Drosophila, and its response to different environmental conditions. Our results from the chi-square analysis data all showed signs of our observed fly count
The fruit fly has an optimal life cycle which makes it convenient and efficient when studying its genetic information. A fruit fly incurs a life cycle that last approximately one and a half to two weeks. Its life cycle manifests in four phases respectively: egg, larva, pupal, and adult stage. Due to the short amount of time it takes a fruit fly to be reproduced and developed, a plethora of information can be learned in a very short period of time (Lewis 1998). Other benefits of using fruit flies are due to: low cost, production in large numbers, and easy production (Lewis 1998). In laboratory, a dihybrid cross was performed in order to determine the traits exhibited by the F2 generation.
This experiment studied Mendel’s law of independent assortment through observing three generations of Drosophila melanogaster. His law was examined by looking at the inheritance patterns that predicted genetic linkage, mapping distances and interference. Genes are located along chromosomes and the distances between them vary. During recombination, these genes may become unlinked. The frequency to which this occurs relies on the recombination frequency, in which a greater value represents a greater distance between two loci. By looking at inheritance patterns and recombination frequencies, this experiment showed that white eyes, short wings and forked bristles are X-linked traits. As well, dumpy wings and brown eyes are autosomal traits. The expected ratio of a dihybrid cross of 9:3:3:1, was used to determine linkage between two loci. This was then verified using a chi-square test. Through analysis of our results, linkage existed between white eyes and short wings, as well as between short wings and forked bristles. Linkage was also found for the strains that had dumpy wings and brown eyes.
Some larva containing vials had hatched into flies. Counting of the flies began at this point. As flies started to grow, at different rates for each vial, with in the first seven days after all larva had hatched the flies were counted. The procedure was done according to theDrosophila manual (45-2620)