Genetic Transformation by Heat Shock

2755 WordsDec 6, 201112 Pages
The Effects of Using the Heat Shock Treatment to Deliver a Vector in Genetic Transformation Introduction: In this experiment we are testing what is required for E. coli to successfully grow on LB (Luria Broth) plates with ampicillin and determining if any genetic transformation has occurred. By combining +pGLO LB and ampicillin we should get an ampicillin resistant gene and by using –pGLO we should create a non-genetic resistant bacteria. The pGLO plasmid has the GFP (green fluorescent protein) gene and the gene that allows the plasmid to be resistant to the antibiotic ampicillin. The most important part of this experiment is the “heat shock treatment” because the E. coli membrane becomes permeable and increases the competency of the…show more content…
Various methods of research proved that E. coli cells when added with tungsten are the most effective projective for plant formation. To test this they experimented with reduced particle size which would in effect improve the binding of bacteria to metal particles, and minimize particle clumping. When tested with corn, the number of transformations was greatest when the bacteria and tungsten particles were air dried onto macro carriers from a watery solution. The results showed that the transformation of tobacco with E. coli projectiles was improved when bacteria were treated with ethanol and ether washes and then dried into a powder. The conclusions of Kikkert (2010) study prove that biological projectiles can be very beneficial in plant transformation because after a DNA construct has been make and then reacts with another microorganism. This eliminates the step of needing to purify DNA in the biolistc process which allows scientists to save time and lowers the chance of DNA cut off. Furthermore these projectiles can be used where high molecular weight DNA constructs are needed. If plasmid containing amp resistance is transformed then bacteria will resist amp and the growth will glow. Like I stated before genetic transformation involves the transfer of genes both between organisms and changes from the exchanging of genes that occurs normally in sexual reproduction. The significance of using genetic transformation is to increase the speed of the
Open Document