Genetics Lab Report

We can also test if there is a significant difference between the average height for females and the average height for the males.

The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in

An association between enzyme production, gene copy number, and gene evolution was explored by conducting analysis of the salivary amylase enzyme, AMY1A gene copy number, and the ancestral starch consumption in Homo Sapiens (Tracey 2017, p.22). It was hypothesized that the relative amount of starch consumption was very high for my personal ancestral diet, thus my AMY1 diploid gene copy number in my genome and salivary amylase concentration would be significantly higher than the population mean. With a population of 28 subjects (n=28), individual saliva samples were collected and compared to a calibration curve to determine the approximate amylase concentration by analyzing absorbance values. Individual samples of buccal cheek cells were

A computer program called FlyLab Software was used to simulate basic principles of genetic inheritance

Analysis As we were conducting our experiment, we collected data so we would be able to determine the differences between males and females. The students then had to take a mini quiz that we had made for the five males and five females, ten students of the age between fifteen through eighteen. We gave the students the quizzes so it would be indiscriminate and not planned. We conducted the quiz three times with each quiz having two questions.

The results show that under selection factors and environmental differences natural selection determines which allele should become more common. In the control simulation the frequency of white alleles to brown alleles, once this mutation was added, was about the same amount. It was almost half white and half brown. In simulation two the environment was an equatorial climate such as a forest and wolves were used as the predatory influence. Once the predatory factor was introduced it can be seen that the alleles of white fur decreased and at the end of the simulation the allele was almost lost. Thus, brown fur alleles were naturally selected in the equatorial environment. The fur color blends in with the environment helping them become harder to find by predators. Whereas, for the white bunnies their phenotype stood out in an equatorial environment causing them to be caught easily. Hence, it can be said that the brown fur alleles had a higher fitness which is why their occurrence was greater and that the white allele was less fit leading to less offspring being produced. Consequently, this supports my hypothesis that the brown fur allele would have a higher frequency in the equatorial environment.

In 1928, Fred Griffith first discovered genetic transformation by infecting mice with unencapsulated and non-pathogenic pneumococci (Lacks 2003). This was the start that opened up the field of biotechnology. Genetic transformation is a process where foreign DNA crosses a membrane of another cell and then alters the genetic material (Encyclopædia Britannica 2015). Genetic transformation can occur in a few different ways: projectile bombardment, electroporation, and heat shock (Weedman 2015). Heat shock is defined by increasing the temperature of cells environment making the plasma membrane become more permeability allowing new DNA to transfer into the cell (Weedman 2015). The cell receiving the new DNA, also known as competent cells, can either amplify the DNA or clone the DNA (JoVE 2015). The most common type of DNA used to perform genetic transformations is plasmids: small, round DNA molecules that still contains two strands of DNA that has the ability

Figure 1 Gel Electrophoresis for Replication Taster PTC. The gel is composed of an ethidium bromide stained 3% agarose gel demonstrating DNA fragments which were a depiction of PCR amplification. The agarose gel contains nine loading samples, including from left to right, the MW marker lane 1 precision mol mass standard, lane 2 TB undigested PTC (5µl of DNA, 5µl of master mix P, and 2.5µl of loading dye), lane 3 TB digested PTC (5µl of DNA, 5µl of master mix P, 2µl Fnu4HI, and 3µl of loading dye), lane 4 TB A(L)DH G (10µl DNA, 10µl master mix G, and 5µl loading dye), lane 5 TB A(L)DH A (10µl DNA, 10µl master mix A, and 5µl loading dye), lane 6 MG undigested PTC (5µl of DNA, 5µl of master mix P, and 2.5µl of loading dye), lane 7 MG digested PTC (5µl of DNA, 5µl of master mix P, 2µl Fnu4HI, and 3µl of loading dye), lane 8 MG A(L)DH G (10µl DNA, 10µl master mix G, and 5µl loading dye), lane 9 MG A(L)DH A (10µl DNA, 10µl master mix A, and 5µl loading dye).

To determine the genotype of the members in my group and the class with regards to the TAS2R38 and LCT genes, first, DNA was extracted from epithelial cells to be used for PCR to amplify the DNA of the targeted loci. Then, restriction enzyme digestion was performed to cut the DNA into fragments in order to perform Restriction Length Polymorphism Analysis to decipher the different lengths. Differing lengths of the DNA accounts for different alleles on that specific gene, therefore determining heterozygosity and homozygosity of the genotype.

A highly conserved gene will be used to identify a prokaryotic species isolated from the body. Fundamental lab techniques will be also explored and utilized, such as amplifying using PCR, cloning, and transforming the gene into a host cell. DNA electrophoresis and specific substrate plating will serve as analysis check points. The final product will be sequenced and compared to similar species to observe phylogenetic relationships.

The debate with-in the anthropology field has been heated over the evolution of the human and the events that have lead us to where we are now. One of the major questions that is debated is how did we, humans-large brained and small jawed, evolve from primates-large jawed and small brained. Interestingly enough, this debate is now being directed from outside the field; by biologists and plastic surgeons. On march 25, 2004, Doctors Stedman (and others) published their findings in Nature (VOL 428) under the title Myosin gene mutation correlates with anatomical changes in the human lineage. Their findings point to the gene myosin and its mutated form MYH16 and

The males were typically seen to be taller in height than the females, but with each member having a different height the trait can vary among offspring. If the environment demands for taller offspring then it will naturally select for that trait among individuals,

that genes and environment must be considered in conjunction during the analysis of traits and

The overall purpose of the lab is to have the students practice designing an experiment, gathering data, and then analyzing that data to form a conclusion using the scientific method. It also served to understand key terms such as hypothesis, dependent variable, and independent variable. The specific objective of this lab is to determine whether certain human body parts experience allometric or isometric growth. Allometric growth defines when certain parts of an organism grow at unequal rates in comparison to its whole, while isometric growth is when all parts of an organism grow at the same rate in comparison to the entire organism. The specific purpose of the lab is to determine whether or not specific human body parts experience allometric or isometric growth by comparing the ratios of height to two specific body parts, in the students’ case the right hand length and head circumference, in students and newborns. The students formulated the tentative answer that if a team of four compared their height to right hand length ratio, as well as, their height to head circumference ratio, to those of a newborn’s, then the students will discover that the right hand and head experience allometric growth in humans.

Genes build the phenotype of humans as well as the underlying genotype. Competition between cultural genes leads to varied success of genetic determinism. It can therefore be said that learnt traits such as those espoused within a specific culture, can produce what may seem to be the genetic genotype of an individual. Genes are not always advantageous in the