Glucose Effect on Diabetic and Non Diabetic Blood Essay example

Figure 2 includes the data points for the absorbance values found using the spectrophotometer of the known standard concentration values that are listed in Table 1 above. A best fit line was drawn in order to determine a representative equation of absorbance and concentration. Both the 1.00 mg/ml and 2.00 mg/ml concentration values were omitted when configuring the best fit line because of their inconsistency with the other data. The line can be defined as y = mx + b, where y is absorption, m is slope, x is concentration, and b is the y-intercept. The slope was determined using y = 0.77 and x = 1.50, yielding a slope value of m = 0.51. Therefore, the equation of the line is y = 0.51x.

Introduction: The goal or purpose of this experiment was to determine the concentration of Allura Red in red commercially available beverage- Gatorade. Colorimeter are used to shine a LED light through the solution and hit a photocell: it will detect an absorbance or a percent transmittance value. These “value” can be charted and examined as a calibration curve. Calibration curve is a method for determining a substance concentration in an unknown sample

The investigation was to determine how an ingested substance can affect urine volume and colour. The volume graph clearly indicates the average volume of urine was highest for participants who consumed water, followed by the electrolyte drink, chips and control who did not consume anything. Each colour graph shows the frequency of urine colour for each group. Colour charts were used to measure the colour of urine, one being the lightest or most dilute and five the darkest or most concentrated. The control group showed increases in frequency for colour one, occurring at 30 and 60 minutes, steady results for colour two, decreases in three at 60 minutes and slightly staggered results for four and five. Partakers who ingested chips

Blood samples were drawn between 7 and 10 a.m. after the fast, waited for the coagulation, and centrifuged for 15 min at 1,500g in the room temperature. Each serum was then aliquoted, stored in the -70 o C storage, and used for the assay of glucose,

Type two diabetes mellitus is a disease characterized by the body’s inability to regulate glucose in the bloodstream. This disease affects millions and can lead to other chronic illnesses and eventually death. An experiment was done to determine the blood glucose of diabetic and non-diabetic individuals over two hours following a meal. A spectrophotometer was used to detect the light absorbency of solutions that represented non-diabetic and diabetic blood, taken right before a meal and thirty minutes, sixty minutes, ninety minutes, and one-hundred-twenty minutes after a meal was eaten. A higher light absorbency indicated a higher blood glucose concentration and a lower light absorbency indicated a lower blood glucose concentration. The diabetic blood samples were found to have higher absorbencies than the non-diabetic blood samples. Those high absorbencies, which also represent high concentrations of glucose, appeared to remain elevated for a longer period of time in diabetics rather than non-diabetics, and it took a much longer time for the blood glucose levels to return to normal in diabetics, still elevated after even two hours. This excess glucose in the blood stream ultimately leads to dozens of other, chronic health problems, but the disease can be prevented or even cured with proper treatment, education, and lifestyle

The patients’ blood is drawn on two separate occasions.” Blood fasting glucose is the best indicator of blood homeostasis and is the preferred method of diagnosing diabetes.” Postprandial blood glucose sampling is also another test done to determine blood glucose level.” Postprandial testing samples are drawn every two hours after a meal to detect the efficiency of the insulin. “Oral glucose test that is done by drinking 8 ounces of a sweet liquid that will also measure the blood glucose levels checking after one hour and then again after two hours.” Normal blood glucose levels should be 70-125

This experiment was conducted to find the absorbance of light by blood glucose samples taken from nondiabetics and diabetics taken before a meal and every thirty minutes for two hours after the meal. The hypothesis for this experiment is that diabetics have a greater absorbance than nondiabetics because diabetics cannot properly regulate their glucose levels. The samples were taken before a meal then taken every thirty minutes after the meal over the course of two hours. This samples were then put into a spectrophotometer to get the absorbance of each. These values were recorded and averaged with the absorbance values from other groups within the lab. The absorbance values show that the diabetic blood glucose samples have a

A capillary blood sample is used to test the blood glucose level (Kucia 2015, p. 875). The normal level is 3.9-6.1mmol/L (Sanchez 2014, p. 582). This is measuring the glucose levels in millimoles per litre of blood (Sanchez 2014, p. 582). As type two diabetes can cause hyperglycaemia, it is important to monitor blood glucose levels (Porth 2015, p. 806). The patient is also taking insulin and medications for his diabetes so it is safe practice to

The absorbance of the beverage solution was then ascertained, and was calculated against the trendline for the scatter plot to determine whether it was within the same range of standards as the food dye. If the beverage had not been within the same range of standards, the beverage would have been diluted, recorded, and measured

The purpose of this experiment is to test and measure the time taken for blood glucose levels to rise after an increase in amount of glucose in the blood, and then the time taken for blood glucose levels to then return to within a normal range. This will be done by using lucozade to elevate blood glucose levels. It is then expected that the blood glucose levels should then return to within the normal range, which in a healthy person should be around 5mmol/L. The rate at which the blood glucose levels return to the normal range depend on the cells ability to absorb the glucose from the blood, which depends on the synthesis and use of insulin. The same test will be carried out with water instead of lucozade in order to obtain

Various organizations have defined prediabetes with criteria that are not uniform. According to World Health Organization (WHO), high risk of diabetes is related to two specifically defined states, impaired fasting glucose (IFG) defined as fasting plasma glucose (FPG) of 6.1-6.9 mmol/L (110-125 mg/dL) and impaired glucose tolerance (IGT) defined as postload plasma glucose of 7.8-11.0 mmol/L (140-200 mg/dL) based on a 2 hour oral glucose tolerance test (OGTT) or a combination of the two (1). The American Diabetes Association (ADA), on the other hand has the same cut-off value for IGT (140-200 mg/dL) but has a lower cut-off value for IFG (100-125 mg/dL) and has additional hemoglobin A1c (HbA1c) based criteria of a level of 5.7-6.4% (2). Several studies have shown poor correlation between HbA1c and IFG and IGT (3-5). The usefulness of diagnosis diabetes or pre-diabetes on basis of IFG and IGT have been challenged due to inability of these blood glucose cut points to capture pathology related to diabetes and probability of developing diabetes in future (6). These cut-offs further loose their credibility due to poor reproducibility of these tests in

The retort stand and clamp were used to suspend the tubing and funnel in the beaker with distilled water (see diagram) 6. The glucose level was accurately marked with a marking pen on the funnel. The glucose level was not to be disturbed during this process. 7. A glucose test strip was used to test for any glucose in the water.

Blood glucose levels and their regulation can be influenced by alcohol (ethanol) consumption. Although consuming alcohol can initially create a rise in blood glucose levels because of its carbohydrate content, excessive alcohol consumption can cause blood glucose levels to drop to dangerously low levels. When alcohol is consumed, it is absorbed directly into the bloodstream from the stomach and intestines; and then travels to the liver to be metabolised. Liver cells contain enzymes which metabolise the alcohol; they break down the alcohol into other chemicals which are in turn broken down into water and carbon dioxide. These are then excreted from the body in the urine and from the lungs. The liver can process only a certain amount of alcohol per

A test that in which a solution with 75 grams of glucose is ingested after 3 days of a high carbohydrate diet and fasting takes place the night before the test, and blood and urine samples are taken from time to time after the intake and lasts up to 6 hours. The blood and urine samples will show if there is the blood glucose takes an unusually long time to be on normal levels.

These two laboratory exams assure me about the condition of glucose in the blood of the patient. Dizziness and excessive sweating, usually show a decreased blood sugar level, which needs to be treated afterwards.