Gmo Lab Report

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Genetically Modified Organisms


The purpose of this lab was to identify if non-labeled food products are actually genetically modified foods. Before we could begin testing this theory we first had to gain an understanding about genetically modified organisms in general. This was rather easy because if you have been to any grocery store lately you have without a doubt seen products with labels saying "GMO-free" or even "contains only non-GMO ingredients." GMO actually stands for Genetically Modified Organisms, and this refers to any products that have been manipulated or altered at the gene level. Modifying plants is not a new concept. "For centuries, gardeners and farmers have been crossbreeding different species of
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To perform each step listed above we needed certain reagents for each process for DNA Extraction we needed a control non-GMO food sample and a testable food sample. Water and a mortar and pestle to grind the sample. Insta gene Matrix was added to two tubes and a water bath. To perform PCR Reactions we needed DNA template that were going to amplify, DNA polymerase, two DNA primers, four DNA base pair subunits and buffers. For electrophoresis we needed PCR product that we collected, molecular weight ruler, and fast blast for staining.
DNA Extraction- For this experiment we used blueberries as our possible GMO food and were given a non-GMO of certified grain as our Non-GMO test sample. Before handling any samples we first needed to label two screw cap tubes with our names, date, and non-GMO and test. After labeling 500μl of InstaGene matrix was added to each tube. A mortar and pestle, that was cleaned with a mixture of bleach and water was used to maintain a sterile environment for the non-GMO food. Weighed out 1.95 g of non-GMO certified grain and placed them in the mortar. Using a transfer pipet added 5ml of distilled water for every gram of food. (See calculations part 1) Grinded the non-GMO certified grain with a pestle for 2 minutes until a slurry formed in the mortar. Added another 5ml of distilled water for every gram of food, mixed until it was smooth enough to pipet. Removed 50μl of the slurry and placed it into the screw cap tube that already contained the
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