able to isolate both the gram-negative and gram-positive bacterium. In order to obtain the correct test results and to ensure the right gram mixed unknown is identified there had to be a series of eight biochemical tests performed on the gram-negative bacterium and five biochemical tests performed on the gram-positive bacterium. Introduction The sole purpose behind this conduction was having the ability to understand just how the gram-negative and gram-positive bacteria can be distinguished and
major classes of bacteria that contain peptidoglycan, Gram-positive and Gram- negative. Gram positive bacteria will stain dark purple due to their thick peptidoglycan cell wall, while Gram negative will stain pink due to their thin peptidoglycan cell wall. Many Gram positive and negative bacteria are considered pathogens because they are capable of causing disease in humans. In order to treat properly individuals who are infected with a pathogenic Gram positive or Gram negative bacteria, the identity
_________ (50 Possible) Title: Identifying a Gram Positive and Gram Negative bacteria from a mixed broth Abstract: I inoculated a T-Soy agar with bacteria number 118, for this I used a streak isolation method. Next, in order to distinguish between Gram positive and Gram negative I used a streak isolation technique on a CNA plate, then performed the same exact procedure on a MacConkey plate. The results from the CNA plate showed the Gram Positive bacteria was an Alpha hemolyzer. Next, I used a P
After conducting the Gram stain to ascertain that the unknown bacterium was a Gram negative bacilli, the species were inoculated in multiple biochemical tests. The tests that led to the conclusion of a Gram Negative bacteria were Oxidase, lactose fermentation, MR, VP, Indole, and H2S production. For the oxidase test, there were two categories one was for positive and negative results of the test. The positive result had three different types of bacteria while the negative result was the Enterobacteriaceae
between Gram positive and Gram negative bacteria. We also used this lab to examine our mixed culture, which had been Gram stained, under the microscope. Theory and background The bacterial cell wall is the outer layer of the cell that aids in structural support and protection from the outside environment. Bacteria can be identified by the structure of their cell wall and classified into two groups known to have different cell wall types. Two of these types are referred to as the Gram positive and Gram
Inhibition Zone Growth of Escherichia coli, Staphylococcus aureus, and Enterococcus faecalis Introduction The variety of bacteria that lives in our world today is endless. With variety of bacteria comes a numerous amount of adaptations and enhancements that the bacteria develops, including antibiotic resistance. Escherichia coli, better known as E.coli, is a gram-negative bacteria with several different types of clones with a variety of effects. E.coli often enters the intestinal tract as soon as an
Introduction: Identification of bacteria is important for a verity of reasons, determining if a bacteria is normal flora, or potentially pathogenic as well as determining the bacterial source of an infection or outbreak. Identifying the bacteria is crucial when treating a bacterial infection because the correct antimicrobial or antibiotic must be used to successfully eliminate the infection and this cannot be done without the identity of the bacteria. Selecting the correct antibiotic is critical
different contexts. Different kinds of bacteria are affected to different degrees by different antibiotics, and it may therefore be useful to know which antibiotic that attacks the bacteria. The purpose of this report is to analyze how antibiotics including Chloramphenicol and Ampicillin affect the growth rate of bacteria Escherichia coli and Bacillus subtilis. Which antibiotic is the most effective or least effective in inhibiting the growth rate of these two bacteria respectively? In the article Growth
to what the result may be. Upon obtaining the unknown organism, it was important to make a streak plate of the bacteria on TSA. The purpose of doing so ensures that we have pure cultures of the unknown to be used in further testing and not a mixed culture. The first test used was a gram stain. It is a differential stain that helps distinguish between gram-positive and gram-negative
The identification of an unknown bacterium was done by streaking for isolation on a Trypticase Soy Agar (TSA) plate then by following the Gram stain protocol described in “Stain Protocols” by Smith (1). both unknown bacteria were labeled as letter A but then given nickname to describe their morphology on the TSA plate. The specific steps of the gram stain and other test that were used and further information was drawn from the documents “lab 1 microscope, inoculation, isolation” that were provided