Gram Staining Paper

11. Clean out your beaker, pipette, and petri dish using water and a paper towel. Put the petri dish back on the supply cart, and put away the beaker and pipette.

Place a small drop of sterile media onto a microscope slide. Take a small sample of the bacteria from the colony using your inoculating loop. It is important that you use asceptic technique when sampling the bacteria as you will be looking at a pure bacterial sample. Flame your inoculating loop as shown by the instructor and allow the loop to cool without waving the loop it the air. When you sample the bacteria make

As soon as the water was added a timer was set for two minutes and the slide was placed under the microscope.

In order to conduct this experiment you will need to know all the major steps. First, you will grab the depression slide and use your eyedropper to collect a single Daphnia and place it in the indentation of the depression slide. Make sure to remove excess water with the corner of your small paper towel. No coverslip will be necessary.

it was previously streaked and slide it across on another side of the agar plate. Then using the

Gently run the swab the surface of one of the agar plate’s labeled landside. Be sure not to touch the agar twice in the same place.

Bio Pre Proposal I. OBJECTIVE: To have the ability to identify gram positive and gram negative cells and understand how the staining affects them. II. HYPOTHESIS: if a smear of both Escherichia coli and Staphylococcus epidermidis are stained, then gram negative and gram positive stains should result respectively and be identifiable under the microscope due to the structure of the cell walls. III. PROCEDURE: 1.

Hypothesis: If the bacteria turns out pink/red then the gram test is negative. Null Hypothesis: If the bacteria turns out pink/red then the gram test is not negative. Purpose: Identify the three morphological types of bacteria and use the Gram’s test to examine the bacteria infection by the stain technique. Differ between plant and animal cells and the different cellular components of eukaryotic cell.

2. Cover the top of the tube with parafilm and invert the test tube 2-4 times to mix the amylase-starch solution. One member of your team must start a timer as soon as the mixing begins. Proper mixing is critical!

Use one loop to streak the two positive plates. Use a second loop to streak the two negative plates.

0.5 μg/ml). Swirl and mix. • Position and appropriate comb of appropriate size, 0.5-1.0 mm over the plate to form complete well defined wells. • The pour the warm agarose solution on the mold. •

Step 2(PART 1 & PART 2): PART 1: Each student takes a slide. Each student also needs one cover-slip and filter paper (for excess liquid) PART 2: Read what you are to do before you do it.

First, The egg will be placed in vinegar for two days to expose a semipermeable membrane. All changes will be recorded. Next, egg will then be placed in corn syrup for twenty four hours, and all changes will be recorded. Finally the egg will be placed in water for twenty four hours and any changes will be recorded

repeat step 3 6. Move microscope aside and add Oil immersion, leave for a few

A gram stain is a simple way to begin differentiating an unknown bacterial sample. Bacteria may either be gram-negative or gram-positive. Staining makes bacteria cells visible, and this particular staining method allows us to narrow down whether the bacteria in gram-negative or gram-positive. Gram-negative stains purple and gram-positive stains pink.