Green Fluorescent Protein Lab Report

When faced with a crisis of identity, we often stick with those who care in the best position to support us. In Neil Smith’s “Green Fluorescent Protein,” When Max was fighting with Ruby-Doo; he wants to stay with his mom for comfort. GFP is a metaphor for Max’s realization that he is queer. Max is ensuring of his feelings towards Ruby-doo. “In Pop’s arms, the white guinea pig turns a brilliant green…… It’s very weird and really scary. [it’s] kind of beautiful in an unexpected way” [50]. He tried to say same sex love can be weird and scary, but it’s love and love it a beautiful thing. Family plays an important role in max’s life. This is what for example Max’s said “I want to say, don’t go. Not that I’m afraid her date

Only after evolution did DNA take over. Proposed by Sir Francis Crick, he determined that RNA could act as an enzyme and a gene to masterfully create the basis for all life today.

1. EMG 9 and EMG 26 contain strain _lac-_(I- Z+ Y+) and strain _lac -_ (I+ Z- Y-)respectively.Three genes huddled together on the chromosome are required for two strains of _E.coli_ to utilize lactose.Consisting of three genes, namely, _lacZ_, _lacY_ and _lacA_, the _lac_ operon orderly handles these genes to code specific enzymes necessary for the metabolism of lactose. The genes _lacZ_, _lacY_ and _lacI_ would code for beta-galactosidase, galactosidase permease and _lac_ repressor respectively. Regulation of _lac_ operon is also tight and the operon's negative control is made possible by _lac_ repressor (Hill, 1996). With the presence of _lac_ repressor, _lac_ operon will be deactivated and will

Green fluorescent protein (GFP) comes from the jellyfish Aequorea Victoria is rare proteins with high fluoresce and absorbance. The purpose this experiments is to purify and express a His2-tagged recombinant from of GFP (rGFP) from the E. coli strain BL21(DE3)< pRSETA-GFPUV > through a series of experiments by using Ni+2 agarose affinity chromatography technology. The GFPuv gene (UV-optimized GFP) was over expressed in the E. Coil strain BL21 (DE3) (pLysS) as an n-terminal His6/Xpress epitope tagged bind protein. Then using Ni2+ Agarose affinity chromatography to obtain purification of the crude extract. Then observe under the long wavelength UV light, the activity of the rGFP in the column fraction. Bradford assay was performed to obtain the total protein amount. When calculating the

In the pGLO Bacterial Transformation lab, Escherichia coli is transformed with a gene encoding green fluorescent protein by inserting a plasmid containing the GFP gene, beta-lactamase, and arabinose into the bacterium. Successfully transformed bacteria will grow in the presence of ampicillin and glow a bright green color under ultraviolet light. The sugar arabinose is responsible for switching on the GFP gene in the transformed cells, without it, the gene will not be expressed.

The hypothesis for this situation is that the plant that is not doing very well is that it is not getting the same amount of sun as the plant that was doing really well. Another possibility is that it’s not getting enough water as the other plant so it could not be doing as well because of those two

Chemiluminescence is a reaction well known to those who enjoy crime scene shows. Luminol is first synthesized in the experiment. Then, the crude luminol produced is oxidized to release photons giving off a light blue glow in a dark room. In a crime scene show, the blood acts as a catalyst aiding in the oxidation of luminol and hydrogen peroxide resulting in the blue glow 1.

Staining the cells with dye allows us to visualize the structures of the cell more precisely under a microscope.

that gene supplied or not. If there is no dominant gene showing up, then they will show through

The final project of the Spring 2018 Foundations of Biology lab focuses on investigating variation in a population. As a class we study population variation to better understand evolution and the mechanics behind it. What is the basis of evolution? The basis of evolution is the differences in the alleles carried by the members of the population in their genome. The differentiation in allele frequency accounts for a majority of the observable hertable differences in a population. Specifically in this project we are anaylzing variation in the population of our Foundations of Biology class. The main objective of this experiment is to investigate the genetic variation in the Foundations of Biology class by looking at two specific

In the past decades lasers have become evidently important to society. Lasers are normally perceived as devices used by our hands or large boxes found in research laboratories. Another evolvement in science that has taken place is the use of fluorescent dyes. Fluorescent dyes have become a common use for research and diagnostics in order to identify specific cell and tissue types. The use of such a dye is to identify for example, various chemical substances such as proteins, tag cells and DNA and their concentration. The only problem with such dyes is only as few as 10 colours have the ability to be distinguished. These newly developed lasers formed by fat cells are a combination of solid styrene beads. The purpose for this evolution is to capture light that is taken from the cells which are views using a spectrometer and thus, analyze the spectrum. It serves a function of measuring refractive index change. Before this step forward the ability to tag cells stood at around a couple hundred cells. Fat cells already consist of lipid droplets and act as natural lasers as it is. They need nothing more than to be supplied with nontoxic fluorescent dye. This new laser technology can help us further understand the cellular process and further improve medical diagnosis and therapies. Considering the level of

Introduction The purpose of this lab was to find the concentration of protein in each solution by establishing a standard curve and to determine the protein concentration in a BSA sample that had an unknown concentration. A spectrophotometer was used to measure the absorbance in solutions containing protein. The spectrophotometer stipulated the absolute protein concentration in the solutions. Hydrated copper sulfate, potassium hydroxide, and potassium sodium tartrate are the crucial components in the Biuret reagent (Biuret Test, n.d.).

According to the newest findings, the ribose sugar from Ribonucleic Acid (RNA) is likely to exist on foreign bodies throughout the universe and may be more prevalent than original belief. These new ideas have the potential to affect not only the study of life on Earth, but also life all throughout the cosmos. It was common knowledge that several biomolecules are clearly present in space, but ribose had been very subtle in our awareness – until now.

Then the second part of the secretory pathway takes place by sending these modified proteins to the Golgi complex by packing them into anterograde vesicles. These vesicles bind to the cis-cisternae of the Golgi, then through the process of cisternal maturation, move across the medial Golgi, to reach the trans-Golgi. As a protein moves through the Golgi complex before it reaches the trans-golgi, further modifications can occur. (Specifically the carbohydrates that were attached to the proteins in the ER can get further modified by glycosyl transferases. The Golgi can also perform O-linked glycosylation like the ER).

Initially the early endosomal membrane, tube-like in shape (Beach, A. et al, 2014), buds inwards via intraluminal invagination (Lopez-Verrilli, M and Court, F. 2013), randomly engulfing sections of the cytosol contents such as the peripheral and transmembrane proteins. This formation of the IVL’s noticeably augments as the endosomes mature (Huotari, J and Helemius, A. 2011) and after transformation the late endosome is more spherical in shape (Beach, A. et al. 2014). It is known