Green Light Hypothesis

Background: The wavelength of light is the distance between the crest to crest, or trough to trough. Studies have shown that red lights incident absorbed the most after the reflected light was absorbed into the plant 's chloroplasts. This led me to choose the color of red for the cellophane which I thought would be the best choice

The spectrophotometer was turned on 15 min prior to the experiment. For each different intensity levels, 2 cuvettes were prepared (one for the experimental variable and one for the control variable). Also, 2 cuvettes were prepared for each different wavelength. Two blanks were prepared for the entire experiment (one for intensity, one for wavelength). The experimental cuvette for intensity consisted of 2.5 ml of 2.5 ml DCPIP, 2.0 ml water, 2.0 ml PO4 buffer, and 0.2 ml chloroplasts, a total of 6.7 ml. The control cuvette for intensity was the same as the experimental cuvette for intensity. The experimental cuvette for wavelength consisted of 2.5 ml DCPIP, 1.7 ml water, 2.0 ml PO4 buffer, and 0.5 ml chloroplasts a total of 6.7 ml. The blank cuvette intensity contained 4.5 ml of water, 2.0 ml of PO4 buffer, and 0.2 ml chloroplasts for a 6.7 ml. The wavelength blank was composed of 4.2 ml water, 2.0 ml PO4 buffer, and 0.5 ml chloroplasts for a 6.7 ml. Before starting the experiment, we set the wavelength at 600 nm, placed the blank cuvette into the spectrophotometer, and set the absorbance at zero. The laboratory was kept dark during the experiment to prevent light pollution.

According to Oregon State University, there are three types of classes that plant pigments can fall under. They are, chlorophylls, carotenoids, and anthocyanins (Carol Savonen 2003). Often the pigments that fall under the category of chlorophyll look green to the eye. This is because they are absorbing all colors of the spectrum except for green. Next, the pigments that are known as carotenoids are going to look yellow or orange, due to the same reasoning. They are absorbing all colors but yellow and orange. Lastly, the anthocyanins will absorb blue and green, reflecting either purple or red (Volner Silva 2016). As the chromatography takes place and the solution is separated into its component substances, they will display the colors of the specific pigments present in them. For example, if Chlorophyll A is present in a leaf, the chromatography will show a blue-green color, while if xanthophylls, another type of plant pigment, are present, the chromatography will exhibit a yellow color. On the other hand, if the anthocyanin pigments are present in the leaf, the chromatography experiment should have resulted in red, blue, purple, or magenta colors (Joy Alkema and Spencer L. Seagerl 1982). Specific to this experiment, the green leaves should display more pigments under the chlorophyll category, while on the other hand, the darker leaves should display more pigments that fall

How does the power output and the number of photons that are emitted depend on the color?

Using the same materials the flask was filled up with 10 mL of bromothymol blue solution and 150 mL of aquarium water. The difference in this solution is that carbon dioxide was not exhaled through the straw and into the flask. The rest of the steps were the same as the first procedure. Data was recorded of the color of the solution in the four test tubes in day one, table two. The new four test tubes were put in the rack along with the last four and the rack was placed under an artificial light source.

It showed us which wavelengths of light each pigment was able to absorb. However, the pigments did not have much variation in their absorbances. All pigments except pigment five were able to absorb large amounts of light in the four hundred to five hundred nanometer ranges and then increased absorbance slightly around six hundred sixty nanometers. The pigments had minimal absorbance between the five hundred and six hundred sixty range.

To understand pigments and their part in the essential process of photosynthesis, we constructed an experiment to discover first-hand the effectiveness of specific pigments found in pimento leaves. These two exercises would specifically focus on the difference in polarities and the different wavelengths at which each pigment absorbs light. The ultimate source of energy for most organisms is sunlight. This research expresses the importance in understanding the driving force behind crucial photosynthetic organisms that are responsible for converting solar energy into chemical energy and ultimately the fixation of carbon dioxide. The polarity of three of the specific pigments studied, chlorophyll a, chlorophyll b, and xanthophylls, was determined by separating the plant pigments by paper chromatography and calculating their Rf values. Once the pigments separated along the paper chromatography strip, we cut the different pigments bands and eluted them from the paper into a beaker filled with acetone. We were then able to analyze the wavelengths of light absorbed by the pigments through the use of a spectrophotometer. We predicted the wavelengths for chlorophyll a, chlorophyll b, anthocyanidins, xanthophylls, and carotenoids. From the first experiment we were able to determine that xanthophylls, which traveled the farthest of the three, was

2b. 650 nm , the first group, will have an intermediate rate of photosynthesis out of all the groups. This is because a 650 nm wavelength is relative to a red color light which is absorbed by the organisms containing the pigment from graph 2/ chlorophyll a, but only produces an intermediate amount of energy for photosynthesis. 550 nm, the second group, will have the lowest rate of photosynthesis out of all of the groups. This is because a 550 nm wavelength is relative to a green color light. Green colored light is either transmitted or reflected by the pigment from graph 2/ chlorophyll a. There is the least amount of energy for photosynthesis at this wavelength. 430 nm, the third

The photosynthetic rates of Camomba Caroliniana, were effected by the color of light it was exposed to during the experiment. As seen in Table 1, Camomba Caroliniana that was exposed to green light had an average photosynthetic rate of 0.13 mL O2 produced/g/hour, which was lower than the photosynthetic rate of Camomba Caroliniana that was exposed to white light (0.19 mL O2 produced/g/hour). The data from table 1 can also be used to determined the reliability of the experiment, the standard errors for green and white light are relatively small, 0.02 and 0.01, respectively. This means that there is less variation in the experiment, but the experiment involving white light is slightly more reliable because its standard error is smaller. The 95%

Based upon the results in experiment 5 the hypothesis for this experiment was not supported because each pigment received a different solubility. To test if solubility was present the pigments would change different colors. In the experiment, the the carotene resulted if having the largest section of pigment a the height of 0.643mm. This explains that each chlorophyll pigment will result in having a different solubility rate. For instance, chlorophyll a and b both received different colors making the hypothesis not true. Chlorophyll a adsorbed green and blue while chlorophyll b absorbed yellow and green. Only sharing a similar color green which is the color of normal chloroplast. The experiment above applies to biology because of the the pigmentation colors absorbed by the sunlight. Biologist can use this data to interpret different solubility rates, which can further into what other types of light conditions will cause the chloroplast pigmentation to be soluble within color change (Gunstream,

Chlorophyll a had the highest absorbance rate resulting in readings peaking at 0.7. Xanthophyll had one of the lowest absorbance reading peaking between .2 and .22. Carotene had the second highest absorbance reading at 0.44 and the wavelength of 460. Chlorophyll b had a very close reading to that of carotene resulting in a peak between 0.38 and 0.41. Within the data of chlorophyll a and chlorophyll b, one will notice a general pattern of increase, decrease, and lastly a smaller increase. Carotene and xanthophyll have only one major peak within the absorbance

Have you ever wondered why some people see an object and say that it has a different color than the original? Our brains are equipped with two wonderful visual receptor cells that help us densifier the differences in color. According to professor King, those receptors are called: Rods (they have a black and white type of vision), and the Cones (have color identification receptors). With these receptors we are able to predict what an object's color is. You've probably seen the "dress" that everyone has been talking about, but if you haven't don't worry you're not missing much.

These show which wavelengths of light the pigments absorb. A beam of light passes through the liquid in the cuvette to the other side, where a sensor calculates how much of the light was transmitted. Absorption is given in conjunction with transmittance. For this experiment, the questions being posed are which is the most active pigment in absorbing energy and which wavelengths of light each one absorbs. Based on prior knowledge, it was predicted that “If the pigments are separated, then Rf values can be calculated; if a pigment is more active in the process of photosynthesis, it will absorb more light across the visible light

Violet light enhances the color, taste, and aroma of plants. Ble light increases the growth rate of plants. Green light enhances chlorophyll production and is used as a pigment for proper plant viewing. Yellow light exhibits less growth compared to blue and red light. Red light, when combined with blue light, yields more leaves and crops,

For lab 12, it is hypothesized that chlorophylls a and b are present in a plant leaf and contribute to the starch production in photosynthesis. Also, products of photosynthesis will be present in leaf tissue exposed to red and blue light wavelengths for several days, but a decreased presence in leaf tissue exposed to green and black light wavelengths. In lab 13, it is expected that since chlorophylls a and b are more polar and smaller molecules than the anthyocyanins and carotenoids, they will travel higher up the chromatography paper than the other pigments.