Green Water Backwashing

Moisten filter paper with a few drops of solvent and turn on water vacuum to fullest extent

8. Repeat step 7 with H2SO4, except that you should use a 10 mL graduated cylinder of H2SO4 and adding 15 mL water.

After all results have been recorded, pour all of the solutions down the drain, rinse equipment and clean up the

5 Remaining in the darkroom move print to tray of running water, do 30-45 second rinse. Squeegee print, place on flat tray.

Fill a test tube about 1/3 full with cold tap water for use in step 34.

Again, label 7 1.5ml tubes 0 thru 6. Place 15μl of each serially diluted extract into its corresponding labeled tube. Next add 465μl of media into each tube. Then 60μl of Alamar blue in each tube. Finally add an additional 60μl of cells (adjusted to 10,000 cells/20 μl). Vortex each tube for 5 seconds. Now, take 3 different samples 190μl samples of concentration 0 and put it in Wells A2, B2, and C2. Repeat this step again by taking 3 more different 190μl samples of concentration 1 and putting it in wells A3, B3, C3. It should be noted that it is important to vortex each 1.5μl tube again be-fore putting it into the 96 well plate. Contin-ue this same procedure consecutively for the re-maining concentrations.

Finally disconnect the tubing and remove the filter paper collecting the solid that has formed on it then allow it to dry

The water used may also pick up impurities from the sulfur so must be purified before being recycled as these impurities can also affect the ecosystem the water is released into.

Repeat steps 3-4: Fill 1 tube with 50% NaCl. Weigh each of the tubes/cells. Record on the chart. 3. Label a 500mL beaker with 50% NaCl and fill it with 400mL of the 50% NaCl solution.

Firmly grab the sides of the filter and gently squeeze the collected residue from the pond water, so that you can add a drop of the water from the residue to a clean glass slide. Carefully apply a cover slip to the slide.

STEP 1: Put small amount of solution to an empty bowl (You can add small amount of water too).

Even through filtration shows its effectiveness, biological and chemical treatments still a significant process as greywater is required further treatment to remove organics, nutrients and surfactants in greywater (Li et al., 2009). In terms of chemical treatments for greywater, there is limited studies have been done so far, only a few technologies such as electrocoagulation (Li et al., 2009). According to Vakil et al. (2014), Electrocoagulation (EC) known as an electrochemical technique, when water flows through the electrocoagulation cell, caused multiple chemical reactions happen simultaneously, as shown in Figure 4. First of all, a metal ion is delivered into water, after that water is hydrolyzed into hydrogen gas and hydroxyl groups on

After the hour has passed, remove the tubes and add 2-3 drops of biuret reagent to each tube and shake.

Thirdly, the proceeding water treatment step involves coagulation and flocculation. The objective of this step is to produce particles of a size that can be easily removed by settlement and filtration. Coagulation destabilizes the colloidal particles followed by flocculation whereby larger particles are formed from small particles through collisions. Conley and Evers (as cited in Hendricks, 2006, p. 277) described coagulation as a process that reduces the surface charge

Water purification is the process of removing undesirable chemicals, biological contaminants, suspended solids and gases from contaminated water. The goal is to produce water fit for a specific purpose. Most water is purified for human consumption (drinking water), but water purification may also be designed for a variety of other purposes, including meeting the requirements of medical, pharmacological, chemical and industrial applications. In general the methods used include physical processes such as filtration,sedimentation, and distillation, biological processes such as slow sand filters or biologically active carbon, chemical processes such asflocculation and chlorination and the use of electromagnetic radiation such as ultraviolet