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Group Synthesis Essay

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At the end of the experiment, seven groups also prepared different solutions. The first group was prepared by adding 1 mL of 100 mM HCl to the falcon tube containing 20 mL of PBS and the pH value was decreased from 7.4 which is the pH value of PBS to 6.22. The second group added 1 mL 20 mM HCl into the falcon tube which contain 20 mL PBS and the pH value was reduced from 7.4 to 7.22. The third group added 1 mL 2 mM HCl to the falcon tube containing 20 mL PBS and the pH value was increased fom 7.4 to 7.8. In the three groups, the same volume of different millimoles was added to the falcon tube containing the same volume of PBS. Due to the inhibition of pH change by PBS substance, it is expected that there is not much change in the pH value of the prepared solutions. As the first group used more millimolar substance, it decreased the pH value more than the second group.
In the first two groups the expected situations have occurred. However the expected results in the third group did not materialize.
In the third group, the pH value is expected to decrease due to the …show more content…

The fifth group brought 1 mL 20 mM HCl to the falcon tube which include 20 mL distilled water and the pH value was decreased from 6.65 to 5.48. The sixth group put 1 mL 2 mM HCl in the falcon tube which contain 20 mL distilled water and pH value was reduced from 6.65 to 6.22. The pH value is expected to decrease when acid added into the water, and this is also true in the three groups. The decrease in pH values at different ratios depends on the use of different millimolar substances. The first group received 1 mL of 100 mM HCl and the pH value was further reduced compared to the second and third group. Because the third group used substances from at least a few millimolar, the pH value changed very

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