Growth Curve Report

The solution was heated till boiling and vigorously stirred. 10mL of 10% barium chloride solution was added drop wise from a measuring cylinder.

Yeast can reproduce both asexually and sexually, which makes it very easy to grow in the laboratory, as it is very small in size. Mutant yeast can be easily isolated considering yeast consists of a single cell and can be grown as a haploid or diploid. Diploid cells are formed by the combination of MATa and MAT alpha cells. However, under conditions of carbon and nitrogen starvation, the diploid cell will undergo meiosis to produce four haploid microorganisms. Because haploids only have one set of genes, its allele can determine the corresponding phenotype. By mating the mutants, the genetics can be carried out through replica plating with the YPD plates (1). Saccharomyces cerevisiae is one of the most commonly studied strains of yeast, in

Sequentially, add 100ul from the previous sample to the next e.g. take 100ul from the neat sample dilution into the following 900ul forming 10

Yeast is a microscopic unicellular fungus, their physiological properties have directed their use in biotechnology. Varied forms of yeast are implemented in the creation of breads, beer fermentation and wine fermentation.

3. Advantages of the serial dilution-agar plate techniques are that it allows counting only living cells, and it allows isolation of discrete colonies that can be further processed to get pure culture to study.

After the seven days, the six petri dishes were taken out of the incubator and the amount of bacterial growth was measured using the two measuring methods (percentage coverage and colony counting) explained in the theory section and the results were then recorded in the results table.

cerevisiae were obtained and labeled accordingly. These served as the starting point for the experiment. An untainted YPD plate is retrieved and sectioned into three areas and labeled: “A”, “B”, and “C”. A small sample of each strain of yeast is taken and lightly spread onto its own section of the YPD plate. The plate will then be left in the incubator at 30 ° C for 24 hours. In the time preceding time five more media plates are acquired and labeled according to a key that indicates the type of media it is. After the time of incubation the original YPD plate is then replicated on SD+His+Leu, SD+His+Ura, SD+His+Leu+Ura and YPD plates (YPD being the control). Each plate needs to be labeled in the same manner as the first media plate so observations can be correctly made. Each replica place should also be labeled according to which strains are going to be transferred to it. A cylinder shape block, with a compression ring is used along with a precut section of velvet in order to transfer an identical pattern of the yeast on the original plate. All of the plates are reentered into the incubator for 48 hours, then the new growth is observed and

Bacteria grows exponentially in the first phase of fermentation (acidogenesis phase) along the formation of acids (mostly acetate and

As expected, the hypothesis stated before the experiment was proven correct because the different substrates did have an effect on the rate of fermentation in the yeast cells (Table 2). However, the predictions of how each substrate would affect the rate of fermentation were not illustrated in the results of this experiment. For example, before the experiment it was predicted that the substrate glucose would produce the highest rate of fermentation, but the flask containing glucose along with the yeast cells, flask two, had a lower rate of fermentation than two other flasks.

A yeast is a fungus that can occur as a single cell and that reproduces by budding. An Ascomycete yeast like a Saccharomyces cerevisiae have a very long served as model systems for cellular, biochemical, genetic, and molecular research. (Cheney, W. (2013). Yeast.). The goal or objectives of this experiment is to identify which type of sugar will ferment with yeast and produce the most CO2. There are three main methods of this experiment. First, adding the yeast starter culture to the fermentation tubes that are containing different types of sugars. Second, mixing the solution fermentation tube. The third, placing the six test tubes into the rack in the water bath (Lab Manual). The main observation of this experiment is that us we produce Carbon Dioxide (CO2). Therefore a test tube that is containing a greater volume of yeast, rather than glucose would have the most CO2 production because fermentation of glucose is dependent on yeast.

collection. 25 mL of distilled, DI water was then poured into a clean, dry 100 mL beaker and

My partner and I will measure each plant (in height) with a ruler using centimeters. We will measure the growth of the bacteria by measuring its width and length (in centimeters) and drawing to-scale pictures. We will time how long it takes for the yeast to bubble up in the warm water and sequentially order the solutions from most vigorous to least. After the microwaving portion of the yeast, we will draw to-scale drawings and photos of what bacteria has accumulated on/in the yeast. A line graph and chart will be provided to show the growth of each organism over

After completing the lab and understand our results, we complemented that we accepted our hypothesis. We determined that we were yeast was able to grow higher based

As the growth of fungi were observed and identified according to the morphological characteristic, then microscopic observation is commonly used in identify the structure by observing the mycelia and spore produced. In order to do the microscopic observation, there are several way can be use in obtaining the characteristics of the fungi which is by Slide Culture Preparations and Cellotape Flag Preparations.

While I determined the overall health of the yeast strains, I performed a cell count by using a microscope and a hemocytometer. At the same time you can add methylene blue: which was used to determine the number of dead and alive cells. The dead cells were stained dark blue on the other hand alive cells were pale blue cells and budding cells. When we added the methylene blue to he yeast sample we were determining the viability of the yeast