HM Complexation At The Cell Wall Plasma Membrane Interface Case Study

Hobart and William Smith Colleges. (2017). Lab 04 BIOL 1510 Lab Manual Plant Defenses F2017 - Biology - StuDocu. Retrieved from https://www.studocu.com/en/document/hobart-and-william-smith-colleges/biology/lecture-notes/lab-04-biol-1510-lab-manual-plant-defenses-f2017/1396233/view

Plant cells have selectively permeable membrane that allows selective substances to penetrate into the cell. Cell membranes are consisting of lipid layer that are made up of phospholipids. (Essays, UK.2013). These phospholipids are amphipathic in nature, which have phospholipids head and tail respectively. Phospholipids heads are hydrophilic and tails

In this lab, we are going to learn how the stress of temperature affects fresh beets. We have come to learn that cell membranes organize the chemical activities of cells. All cells are made of plasma membranes, often called fluid mosaics. It is sometimes described as a mosaic because it is made of protein molecules that are embedded into phospholipids. Phospholipids are the main structural support of the membrane and the proteins perform most of the functions of a membrane. Together they form boundaries or barriers between the cell itself and its surroundings, like the membrane of an egg. Plasma membranes also control what substances come in and out and also dispose of the

Despite its importance osmosis may also damage cells by causing them to; a) shrink from water loss or b) burst from too much water gain. Plant cells [fig 3] have adapted themselves to ensure that these factors do not affect them, by forming a ridged wall, known as the cell wall, around their cells. The cell wall maintains the shape of the cell, and prevents the cell from bursting in a hypotonic medium by resisting water pressure. Plant cells have also adapted a larger vacuole, which occupies 80% or more of the cells cytoplasm (Davidson, 2004); allowing plants to store more water and nutrients per cell. Vacuoles also play a structural role in plant cells; by swelling when liquids contact them, plant vacuoles are able to control turgor pressure within the cell. This helps maintain the structural integrity of the cell as well as providing the plant with suitable amounts of water and nutrients; however the cell will never burst because the vacuole is contained within the cell wall. If plant cells are deprived of water their vacuole will begin to shrink, yet due to the cell the wall, the plant cell will be able to maintain its shape. [fig.4] Animal cells [fig 5] on the other hand do not have this

War, A., Paulraj, M., Ahmad, T., Buhroo, A., Hussain, B., Ignacimuthu, S. and Sharma, H. (2012). Mechanisms of plant defense against insect herbivores. [online] NCBI. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3493419/ [Accessed 10 Mar.

A., & Kiss, J. Z. (2012). Phototropism and gravitropism in transgenic lines of Arabidopsis altered in the phytochrome pathway. Physiologia Plantarum, 145(3), 461-473. doi:10.1111/j.1399-3054.2012.01607.x

Many species of plants host microorganisms living inside the plant forming a mutually beneficial endosymbiosis. Bacteria or fungi that reside within plant tissue (roots, stems, and/or leaves) are referred to as endophytes. These endophyte communities may help to improve a plant’s fitness by promoting growth, protecting against disease, or facilitating nutrient acquisition. More specifically, endophytes within the plant community can help plants respond to stress that develops from biotic or abiotic influences like pests, heat, drought, saline, and soil conditions (Russell et al., 2003) Endophytes can help plants become better able to tolerate stress by allocating resources from one place to another (Rodriguez et al., 2009). Therefore,

Plants have evolved sophisticated genetic and epigenetic regulatory systems to respond quickly to unfavorable environmental conditions such as heat, cold, drought, and pathogen infections. In particular, heat greatly affects plant growth and development, immunity and circadian rhythm, and poses a serious threat to the global food supply. According to temperatures exposing, heat can be usually classified as warm ambient temperature

Robert Deal from Emory University is studying to learn about plants and their memory of stress. When plants face dry weather, their stomata shrink to reduce water loss. When a similar situation places the plants under stress again, the plant seems to recall this experience and recovers quicker. Robert Deal, who studies genetics and biochemistry, hopes to utilize this trait and pinpoint its gene. If he can locate and activate the genetic material associated with this memory, he believes he can speed up the process and cause plants to have the gene activated at all times, allowing the plants to withstand drier and warmer temperatures.

Ecologically speaking, the plant life is heavily dependent on dehydration synthesis. The cell wall of a plant is entirely composed of a carbohydrate- cellulose- that was constructed with dehydration synthesis and other nutrients. The cell walls are the leading reason for plants’, and trees’ stiffness. To further expand upon this idea, a maple tree is able to grow tall, partially due to the stiff cell wall. The importance of healthy and strong plant life to the environment should not go unnoticed. More importantly the contributions of dehydration synthesis are indirectly and directly responsible for the above applications, and drive several biological systems. Medically speaking, the absence of proteins has been linked to several health disorders such as Alzheimer's and Creutzfeldt-Jakob disease. The importance of properly constructed proteins is the difference between a healthy functioning brain and a disease that could result in death or other extreme negative side

Na+ , K+ and Cl- cotransporter is envolved in electroneutral transport at apical surface, which is driven by low concentration of these ions. This low concentration of ions is achieved by basolateral Na+ and K+ contertransporter (sodium-potassium adenosine triphosphatase) and basolateral chloride ion channel by facilitated diffusion (CLC-kb). Potassium ion is capable of diffusing back to lumen through apical potassium channel (ROMK) and returns net positive charge to lumen, this is important for reabsorption of calcium and magnesium

The plant recognizes the double stranded mRNA as foreign and breaks down the complex. This acts as a negative post-transcriptional control (Axtell). The four microRNAs used for this investigation were miR156, miR395, miR399 and miR398. The control, miR156, has no nutrient requirement and would be expected to show no change due to nutrient levels changing (Hsieh 2120-2132). miR399 is up-regulated during phosphate starvation and thus targets phosphorus uptake and metabolism (Hsieh 2120- 2132). miR398 is regulated when there is Copper/Zinc starvation, targeting the enzyme copper/zinc superoxide dismutase (Bouché 684– 686). This stress responsive miRNA has low levels of expression in both low sulfur and phosphorus environments (Bouché 684- 686). Lastly, miR395 depends on sulfur concentrations and targets mRNAs involved in sulfur metabolisms (Hsieh 2120-2132). These miRNAs should show different effects due to the nutrients

Plants are the major source of vitamin B6 for animals, yet the biosynthesis pathway and the function of vitamin B6 in plants are not well elucidated. In this study, an Arabidopsis pyridoxine synthase gene PDX1 was characterized and its in vivo functions were investigated. The PDX1 gene was expressed in all plant parts examined and its expression level was not significantly regulated by abiotic stress or the phytohormone abscisic acid. In roots, PDX1 was highly expressed in a defined region behind the root tips that undergoes rapid cell division. The PDX1 protein was mainly associated with the plasma membrane and endomembranes, implying a potential involvement of vitamin B6 in membrane function. To reveal the in vivo role of PDX1, Arabidopsis insertional mutants were isolated. Strikingly, the pdx1 knockout mutants were impaired in root growth and early seedling development. The stunted roots resulted from both reduced cell division and elongation. Supplementation of the growth media with pyridoxine or reintroduction of the wild-type PDX1 gene into the mutants completely restored the mutant growth, demonstrating that PDX1 is required for pyridoxine biosynthesis in planta. In addition to the developmental defects, pdx1 mutants are hypersensitive to osmotic stress and oxidative stress. These mutant seedlings had increased peroxidation of membrane lipids following UV treatment. Our study establishes a

In plants the cell membrane is surrounded by a cell wall. The cell wall is outside the cell membrane, and its purpose is to help the membrane protect and support the cell. Since the cell wall is very porous, water, oxygen,

All plants are subjected to a multitude of stresses throughout their life cycle. Depending on the species of plant and the source of the stress, the plant will respond in different ways. When a certain tolerance level is reached, the plant will eventually die. When the plants in question are crop plants, then a problem arises. The two major environmental factors that currently reduce plant productivity are drought and salinity (Serrano, 1999), and these stresses cause similar reactions in plants due to water stress. These environmental concerns affect plants more than is commonly thought. For example, disease and insect loss typically decrease crop yields by less than ten percent, but severe