Hair Cell Regeneration After ATOH1 Case Study

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Atkinson, P., Wise, A., Flynn, B., Nayagam, B., Richardson, R. (2014). Hair Cell Regeneration after ATOH1 Gene Therapy in the Cochlea of Profoundly Deaf Adult Guinea Pigs. PLoS ONE9(7).
• Purpose: The purpose of this study was to determine whether or not degenerated hair cells within the cochlea could be regenerated using the introduction of ATOH1. ATOH1 is a transcription factor that is known to be essential to hair cell growth and development. Typically, damaged hair cells within the cochlea result in permanent sensorineural hearing loss and this study aimed to see if these results could be reversed using guinea pigs as the study participants.
• Methods: This study used both male and female guinea pigs in which their hearing status was
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Tong, B., Hornak, A., Maison, S., Ohlemiller, M., Liberman, M., Simmons, D. (2016). Oncomodulin, an EF-Hand Ca2+ Buffer, Is Critical for Maintaining Cochlear Function in Mice. Journal of Neuroscience. 36 (5) 1631-1635.
• Purpose: The purpose of this study was to determine if oncomodulin is necessary for cochlear development as well as cochlear functioning. Looking at oncomodulin is a relatively new topic in the scientific community and therefore this research is applicable as scientists are trying to figure out the purpose behind it being located almost solely in the outer hair cells of the cochlea. In order to better understand the function of oncomodulin, this study depleted hair cells of oncomodulin to see how cochlear function was affected.
• Methods: In this study, the genes for oncomodulin were cloned and after sequencing, two blastocysts were injected with two clones. Crossing the two oncomodulin heterozygotes generated an oncomodulin homozygote flox, and this flox was crossed with a mouse under the control of an actin promoter in order to create a mouse where oncomodulin was to be deleted. The mice were then put under anesthesia while their distortion product otoacoustic emissions and auditory brainstem responses were recorded. The cochleas were then microdissected and then antibodies were used to immunostain and study. Finally, confocal z-stacks were obtained from each ear and were imaged using Volocity software in order to see how the
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