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Happy Milk Lab

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Do Happy Cows Make Better Milk?: Analysis of the Chemical Components of Milk The main goal of this lab was to correctly separate milk into its major components. Other objectives included: performing basic qualitative tests to confirm correct separation, identifying the major components of milk, and assigning polar and non-polar properties to components of milk. The goal of separating the components of milk was desirable because their differences in properties required different techniques to separate them. The casein, Vitamins A, D, and E, and fats were all nonpolar and therefore dissolved in nonpolar solutions.1 Whey, lactose, calcium, phosphate, vitamin B, vitamin C, and water were all polar and therefore dissolved in polar solutions.1 …show more content…

The protein was unraveled as disruptions occurred in the protein’s secondary and tertiary structures.2 The result of denaturation was solid whey protein. The lactose, calcium, phosphate, and water were unaffected by the heating process and remained liquid. In the third separation, ethyl acetate was added to dissolve the fats mixed with the casein. Casein, unable to dissolve in the nonpolar solution, remained a white solid.1 The ethyl acetate, with a lower boiling point than the fat, was boiled off, leaving a yellow liquid containing fat and various fat-soluble vitamins. Vitamin A caused the liquid to be yellow, which confirmed the presence of vitamins. The residue was added to water, where it proved to be insoluble in water. This indicated that the residue contained fat, because fat was the only nonpolar liquid remaining at the end of the separations. The tests for carbohydrates, lactose, calcium, and phosphates performed on the liquid portion from the second separation were all positive. This was expected, so no errors were made in the first or second separation. The tests for proteins performed on the solids produced from the separation were both positive, so no errors were made in the separations that isolated casein and whey. The test for proteins performed on the liquid portion from the second separation was negative. This was expected because it meant that all of the whey was removed from this portion in the denaturing

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