INTRODUCTION
The heart is made of specialized tissue known as cardiac muscle. Cardiac muscle is made of myocardial cells which can be further divided into autorhythmic and contractile cells. The autorhythmic cells are composed of sinoatrial (SA) nodes and atrioventricular (AV) nodes. These nodes contain pacemaker cells that control heart rate by producing pacemaker potentials. An example of a pacemaker potential is shown in Figure 1. The potential goes from slow depolarization to rapid depolarization to a peak and lastly to a repolarization phase. In the slow depolarization phase, funny channels, which are special slow sodium channels found in the pacemaker cells, open and Na+ flows in and depolarizes until threshold is reached. In the rapid
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Based on these results, it can be seen that the addition of caffeine increased the heart rate of the frog. The data collected is shown in Table 5. Effect of Pilocarpine and Atropine
Before the addition of pilocarpine, the heart rate was found to be 60 bpm, and after the addition of pilocarpine, the heart rate was found to be 36 bpm. It is also worth noting again that the heart was not rinsed with Ringer’s solution before the addition of atropine. After the addition of atropine, the heart rate was found to be 20 bpm. Based on the results, it can be seen that the addition of pilocarpine decreased the heart rate of the frog and the addition of atropine further decreased the heart rate. The data collected is shown in Table 6.
Effect of Epinephrine
Before the addition of epinephrine, the heart rate was found to be 20 bpm, and after the addition of epinephrine the heart rate was found to be 44 bpm. Based on these results, it can be seen that the addition of epinephrine increases heart rate. The data collected is shown in Table 7.
Effect of
Table 5. The effects of Atropine measured by the ventricle of the frog’s heart by amplitude, period, and BPMs.
After completing the experiment we found that when we gave the Daphnia caffeine the heartbeat rate did show an increase. However, we also found that alcohol also increased the number of times the heart beat. Even though we performed all of the experiments very carefully, we cannot be certain that the effect we saw was due to the drugs. Perhaps the change in heartbeat rate is caused by
The two drugs that have been used in this experiment to test their effects on the heart rate and depolarization rate of the P wave of the crayfish. Depolarization is defined as the contractions of heart muscle, and it is shown in P wave pattern of the ECG. Comparing the results of the trials of epinephrine drug to the resting state, it shows that this drug increases the heart rate, decreases beat period, and decreases the time that take
White PJ & Nguyen TT. 2002. _Chronic Caffeine Treatment Causes Changes in Cardiac Adenosine Receptor Function in Rats_. 65,3, 129-135.
The purpose of this experiment was to see if caffeine had an effect on the heart rate of Daphnia (water fleas) by observing them under the microscope and recording their heart rate over a period of time in concentrations of caffeine. Caffeine, which is a stimulant drug rttused in coffee, tea, Red Bull and Pro-plus, causes increased amounts of stimulatory neurotransmitters to be released. As caffeine is a stimulant, a prediction was made that caffeine would increase the heart rate of the Daphnia.
In order to complete this exercise, two different procedures were conducted; one measured the basal heart rate, and the other measured the drug-induced heart rate. A sample 's basal heart rate can be defined as the “resting” heart rate. This is when no drugs or altering substances are applied. In comparison, the drug-induced heart rate of a sample can be defined as the heart rate after the drug was administered to the surrounding environment. The four drugs tested on the Daphnia specimens were Acetylcholine, Caffeine,
In a normal human being the heart correctly functions by the blood first entering through the right atrium from the superior and inferior vena cava. This blood flow continues through the right atrioventricular valve into the right ventricle. The right ventricle contracts forcing the pulmonary valve to open leading blood flow through the pulmonary valve and into the pulmonary trunk. Blood is then distributed from the right and left pulmonary arteries to the lungs, where carbon dioxide is unloaded and oxygen is loaded into the blood. The blood is returned from the lungs to the left
The purpose of this lab was to determine the normal heart rate of a Daphnia Magna and decipher the different effects that various substances had on it. A Daphnia Magna is a species of water fleas and can be located in the Northern United Sates against the coastline of the Atlantic in rocky pools. The water flea’s habitat consists of rivers and streams, temporary pools, lakes and ponds, and brackish water. The Daphnia Magna range from two to five millimeters in length and are shaped like a kidney bean (Elenbaas, Molly). Relating to this lab experiment we learned in class that the normal heart rate is measured anywhere between 60 and 100 beats per minute (BPM). If your resting BPM is measured at a level above the number 100 it is known as Tachycardia. This term indicates that your heart level has exceeded the normal range. Also if your heart rate is indicated below 60 then it is called Bradycardia, which means the heart is beating slower than normal. When your heart rate is affected by a substance in the body it is called a Chronotropic agent. When the heart rate decreases because of a substance or chemical it is called a negative Chronotropic and when it is affected oppositely by increasing it is known as a positive Chronotropic agent. In this experiment we added many different substances to the slide on which the water flea was placed to calculate the increase or decrease in its heart rate due to the ingredients in the substances. The first substance used was
Subjects running in place for one minute increased their heart rate. Subject 1, --Enmanuel Aponte, active heart rate at 20 seconds was 121 BPM, but at 40 seconds the subjects heart rate skyrocketed to 184 BPM. Therefore, showing a 63 BPM difference. Subject 2, Kelsey Flores, active heart at 40 seconds was 159 BPM, then at 50 seconds the subjects heart rate jumped to 172 BPM. Lastly, the average active heart at 20 seconds is 148.5 BPM, while at 40 seconds it is 172.75 BPM.
The cardiac muscles are the muscle of the heart itself. The cardiac muscle is the tissue that makes
The purpose of this experiment was to identify how open and closed circulatory systems respond to chemical stimulants. During this experiment a ghost shrimp represented the open circulatory system, while the black worm represented the closed circulatory system. For each organism there resting heart rates were recorded by count the number of pulsations the organism had under a microscope. First, the shrimp and the worm were treated with caffeine, and then their heart rate was recorded after the organisms were exposed to the treatment for three minutes. The organisms were removed from the caffeine, and were given time to return back to their resting heart rates. The shrimp and the worm were then exposed to ethanol, and there hear rates were recorded again.
A pacemaker consists of a battery, computerized generator, and wires with sensors called electrodes. These electrodes detect the electrical activity of the conducting cells and then send the data back to the generator via the wires. If the pacemaker senses irregular activity, it will deliver a low energy pulse to the heart. For reference, the current delivered by a pacemaker is about one thousandth the size of the current needed to start a car. Most people report feeling a fluttering in their chest, if anything at all. Another interventional option is the ICD, which acts as both a pacemaker and a defibrillator. An ICD consists of a pulse generator, which monitors your heartbeat, and the leads. The leads of the ICD have electrodes that connect to the heart chambers. If an irregular rhythm is detected, a low energy pulse is delivered to prompt the heart to start beating normally. If the low energy pulse is not effective or sufficient, the ICD switches to a high energy pulse called defibrillation. People report defibrillation as feeling like you are being kicked in the
The chart does not confirm what our hypothesis was: that the higher the concentration of the caffeine solution the more the beats per minute of the daphnia. This, though, might be a mistake since the caffeine solution may not have been exactly as we wanted it in each occasion, hence making the results inaccurate. Also, when trying to press the “=” button of the calculator faster, many of those additions were not calculated since the calculator can’t add as fast as we press the button.
The table above can also be found in Seeley’s Anatomy and Physiology laboratory manual on page 376.
Regular electrical impulses are sent within the conduction system of the heart prompting contraction (Marieb, 2015). These electrical signals can be identified and documented by the use of an electrocardiography (ECG) machine. In a familiar ECG recording, three waves will occur; The P wave, QRS complex and