Heritable Genetic Information In Escherichia Coli In Transformation

1664 WordsMar 31, 20177 Pages
Heritable Genetic Information in Escherichia Coli in Transformation and Deoxyribonucleic Acid A report for Biology 2000 written by Alana Pay : 001196733 Introduction Griffin was a scientist that showed that there was a non-living component to heritability between organisms, although he had not yet discovered what exactly was the heritable component. However, during this time period, it was a common belief that the heritable material was contained within the protein of the organism, not the DNA that is now held in the truth. In the Avery and company experiment, they expanded Griffin research to pinpoint the exact location of the heritable information by breaking down cell components until the final product allowing transformation was…show more content…
The experiment completed by Stanley et al, also used the transformational effectiveness of E.Coli to complete their R-Factor DNA study, so the effectiveness of using E.Coli in this type of experiment is well documented. (Stanley et al, 1972) The antibiotic that was used in our replication experiment was kanamycin, which is a amino-glycoside and it proceeds to block the areas that initiation complexes may occur. (National Center, 2017) A model system allows us to witness the effects of an experiment in many generations and in an organism that grows in a very quick fashion. The genetic growth is generally very similar to that of humans so that we may compare and contrast what may happen in humans, or what will before we start clinical trials of certain chemicals or antibiotics, in this case, that may have a positive or negative impact on the human populace. The objective of this experiment is to conclude that the results of Griffin and Avery et al, can be duplicated in a way that will allow us to corroborate their results. The null hypothesis that this repeated experiment revolved around was that the DNA involved from the E. Coli would not undergo transformation and therefore grow a strain, which means that there would be growth on any of the plated specimens. This leads to the alternative hypothesis that only the plates that involved the DNase would not have growth as it has the heritable genetic information that would allow the transformation to occur and

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