Hgihsc Animal Study Sample

Animal testing has contributed to many life-saving cures, treatments, and major advances in understanding and treating conditions such as breast cancer, childhood leukemia, brain injuries, cystic fibrosis, malaria, multiple sclerosis, tuberculosis, and many others, and was instrumental in the development of pacemakers, cardiac valve substitutes, and anesthetics. Using animals as research subjects is appropriate because they are similar to human beings in many ways.

Rivera, M., S., (n.d.) Research with Humans and Animals: IRB and IACUC Basics, Associate Vice President UT Southwestern Medical Center Retrieved from

From a panel of veterinarians, they all explain how before even thinking of euthanasia as an option, they consider the physical and psychological health of the animal before determining the solution to a case. Dr. Flinchum introduces his method of considering euthanasia when an animal’s quality of life is deteriorating and would then draw a plan up to consult with the owners and would only resort to euthanasia if it is in the owner’s best interest for their pet (Hess 53). Dr. Horton states, “We also have it in our policy that we do not euthanize healthy animals. We do offer placement services or referral to behaviorists for those animals that may just need to be rehomed or retrained” (Hess 54). The method that many doctors use for euthanasia is to administer isoflurane gas through a mask until the animal is in a deep sleep state (American Veterinary 6). After, the chosen euthanasia solution would be administered into the jugular or intracardiac vein on the animal which would then result in a calm and peaceful ending for the animal. Unfortunately, the methods and practices of the doctors gathered on this panel are oftentimes not practiced at other animal hospitals. Instead, the policies of the professionals often do not consider the psychological, mental, or physical state that an animal is in and will implement euthanasia regardless of the animal’s health

This study will be comprise two experiments. The animal trials will be conducted at the Experiment Research Station and the laboratory work in the facilities of the Institute of Animal Science, University of Hohenheim. Field studies will not be included.

use. The Animal Efficacy Rule (21 CFR 314 Subpart I and 21 CFR 601 Subpart H) allows

weakening the immune system so the fungi can make its way to the lungs then

Although there has been thousands of reports of animal abuse in the field of medical testing, there has also been an extraordinary amount of success that come from these examinations. With these tests, scientists were able to advance in

This study was carried out in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The protocol was approved by the Institutional Animal Care and Use Committee at the University of Hawaii, and all efforts were made to minimize animal suffering.

The bacterium is mainly found in wild rodents such as ground hogs, squirrels, prairie dogs and mice (Phillips, 2004). The bacterium is provided a comfortable residence in these rodents where it also has the ability to increase its population or at

Cohen, Carl. “The Case for the Use of Animals in Biomedical Research.” New England Journal of Medicine 315:865-870 (1986). Print.

According to the CDC there are simple steps in preventative measures when dealing with zoonotic diseases. The first is to know what zoonotic diseases are and how they are transmitted. The second is to constantly wash ones hands thoroughly and frequently especially when interacting with animals. Although one may pet their animal on a daily basis, this animal can still carry a virus and precautions should be taken. A major recommendation made by the CDC was to avoid direct contact with certain animal species and their personal environment. This is seen when individuals enter caves that are infested with bats who are one of the main carriers of zoonotic viruses. Another precaution that can be taken with this is not removing animals from their natural habitats in which the living and heath conditions of the inhabitants of that area are unknown. The final recommendation made by the CDC is to properly vaccinate animals against disease. Although this doesn’t help with animals in the wild it prevents animals that currently reside near humans from contacting these viruses and then continuing the spread from these humans to other

Pneumocystis jiroveci is an opportunistic unicellular fungal pathogen that is widespread in the environment and thought to enter the body via airborne transmission. It also represents one of the several microorganisms that can cause life-threatening infection in patients with advanced HIV

The PHS policy on humane care and the use of laboratory animals and The Guide for The Care and Use of Laboratory Animals

A fecal sample inside a swab tube was obtained and stored at -20 C until DNA extraction. DNA was extracted from the sample using the Eppendorf EpMotion 5075 robot obtained from Eppendorf’s main website. Fecal samples obtained were taken out of the -20 C and placed to thaw. 20 microliters of Broad-spectrum serine protease; endopeptidase K and 20 microliters of lysozyme were added. Samples were then placed in a freezer at -80 C for thirty minutes and then taken out to thaw at 40 C for sixty minutes a total of 3 times. 75 microliiters of sodium dodecyl sulfate was then added to the samples and left to incubate in a 65 C water bath for 120 minutes.

To protect the welfare, the animal experiments involved in the study are carried out in accordance with the Australian Code of Practice for the Care and Use of Animals for Scientific Purposes, 8th edition (2013), and the study is assessed by the local institutional Animal Ethics Committee (AEC), throughout the study period.