High Fidelity Optical Reporting Of Neuronal Electrical Activity With An Ultrafast Fluorescent Voltage Sensor

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In the article, “High-fidelity optical reporting of neuronal electrical activity with an ultrafast fluorescent voltage sensor”, an investigation of visual reporting of electrical signals in the brain is discussed. The authors express the importance of optical representation of electrical activity in the brain as an extremely useful tool for various neuroscience applications. This includes mapping the activity of genetically defined neurons in the brain and decoding neuronal communication. In particular, previous methods lacked in a few abilities the authors hoped to improve on by testing their hypotheses. The abilities included spatio-temporal imaging of how input signals are processed by neurons into output responses such as action potentials, tracking high-frequency firing for decoding neurotransmission, fast enzyme kinetics, and developing a way to track membrane potential changes. With these abilities it is possible to map responses in the brain to actions of the body and emotions to help diagnose nervous system responses. Experimental Methods Methods used in this experiment include use of plasmids, imaging techniques, use of HEK cells, and voltage/current clamps, and finally an SNRC calculation. Plasmids are used because they are easy to manipulate and integrate into plasma membranes1. This allows an efficient and quick insertion of desired substances into the desired protein like GFP into ASAP1. In this experiment GFP was introduced into the S3 S4 loop where

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