Histopathology Lab

According to the list of materials, the instruments that will be used in this dissection are scissors, dissecting pins, a scalpel, a dissecting tray, a probe, forceps, dissecting needles, and a cord.

1964). There was a statistically significant effect of time spent in the blocking solution and the fixative and staining quality. In future experiments, the time samples are placed in blocking solution and fixative should be monitored more closely and left for a sufficient amount of time to get better and more accurate results, concerning staining quality. On the contrary, there was a positive correlation between the amount of time the samples were left in the antibody solutions and staining results. The longer the samples were left in the antibody solution, the better the staining results were as attested by the statistical

The tissue that they have gotten will be set to a lab and studied to confirm the diagnosis.

Once the check has been approved an episode number is assigned and signed by two members of staff as well as a count sticker and a time stamp. The physical state of the sample is also checked to detect signs of leakage or an inconsistent anticoagulant volume. This can be reported to a senior so that a new sample can be requested as stated in (FSOP 477). Orange sticks can then be used to detect the presence of clots. If present, the size of the clot can be written down on the sample and its blood count report. 200 micro litres are then placed into Eppendorf tubes. A WBC/differential/NRBC is performed with this sample on the Sysmex EX5000, producing a full blood count report. The report is then labelled with the patient’s episode number, name, clot presence and volume. The sample is then packaged in a sealed wallet with the request form, blood count report and is place within the blue tray labelled P.2013 in

The stain was added to enable the observation of chromosomes as it helped to make them stand out under the microscope.

Biopsy is a small piece of tissue that is collected from the patients using surgery or endoscopy (Figure 3) [12,16].

Frog dissection requires a scalpel and scissors from a dissection kit to begin the process.

3.4 Pro-healing marker (m-CSF) and pro-inflammatory marker (gm-CSF) were used to monitor % positive cells using the flow cytometry.

The doctor prepares it for the flap of tissue containing healthy lymph nodes, by removing a flap of damaged tissue.

Process for drawing out aneurysms: First they seized and raised small pieces of tissue with the

The aim of this technique is to embed tissue into wax. Embedding tissue into paraffin blocks supports the tissue structure and enables to cut very thin sections and mount them onto microscope slides for posterior staining and analysis. I prepared tissue on paraffin sections for staining. This technique is divided in different steps, most of them made automatically by a machine:

The major use of Enzyme Linked Immunosorbent Assay (ELISA) is to titrate antibodies against specific antigens (Crowther, 1995). This method involves an enzyme, which reacts with a colourless substrate to form a coloured product, is covalently linked to a specific antibody that recognises a specific antigen. If the antigen is present, the antibody-enzyme complex will bind to it, and the enzyme component of the antibody-enzyme complex will catalyse the reaction which results in a coloured product (Berg et al, 2002). The presence of the coloured product indicates that an antigen is present. The ELISA, which is rapid and convenient, can detect less than a nanogram (10-9 g) of a protein and can be performed with either polyclonal antibodies, which are antibodies that can recognise multiple epitopes on any one antigen or monoclonal antibodies, which are antibodies that can only detect one epitope on the antigen (Guo et al, 2006). However, the use of monoclonal antibodies gives more reliable and accurate results due to their specificity as monoclonal antibodies are very effective at acting as the primary antibody in an assay, or for detecting antigens within a tissue. Also, they will usually result in reduced background staining compared to that of polyclonal antibodies.

6.3.5.2. Histopathological Analysis Documented Enhanced Granulation Tissue Formation, Angiogenesis, Collagen Deposition, and Neo-Epithelialization by NCs Treatment

(3)"Unsatisfactory for evaluation" designates that the specimen is unreliable for the detection of cervical epithelial cell abnormalities.this term is used if any of the following apply:

Purpose: What is the purpose of this exercise? The purpose of this exercise is to become more familiar with microscope and slides through observations of tissues. By looking at so many slides I am now much more comfortable at identifying some basic structures. The tissue slides were very important to my understanding of the tissue's function. By looking at the dense regular tendon I can see how supportive it is because of all of the fibers present. It connected what I had read and studied to an actual picture and that helps with memorization and understanding.