In contrast the 2% hyperchlorite HOCL dissociates readily to -OCl and H+. Both HOCl and the hypochlorite ion (-OCl) are strong oxidizing agents, known to cause a biosynthetic alteration in cellular metabolism and a phospholipid destruction and also interferes in cellular metabolism and causes irreversible enzymatic inactivation in bacteria essential for the growth, and a lipid and fatty acid degradation. This is associated to the penetration of HOCl into the bacterial cell wall and membrane. HOCl can attack from outside and also from inside the cell thereby hastening the inactivation rate and increasing the bactericidal activity.
Ajax, which was thought to have killed the most E. coli, was because of its ingredient lactic acid, which was used as a natural anti-bacterial. A potential reason why the Ajax didn’t kill as much E. coli as what was predicted might have been that the amount of Ajax solution soaked up was not actually sufficient enough to cause an effect. Therefore, the results varied. The results were different and had a large gap between them causing three varied answers. Vinegar was thought to kill not as much as Ajax but as it turned out, had a larger zone of inhibition but didn’t efficiently kill or clear as much. The vinegar tried to kill more because of the high acid levels that entered the cell membrane and killed the cell. The vinegar that was used didn’t kill as many cells because the concentration was not high enough. Again, the same as the ajax, there were 3 varied answers. This is proposed to be because of different of length of time that it spent in the solution. Salty water which was presumed to kill the least was correct.
There of the dishes turned out as expected in this experiment. Our group expected there to be growth in the LB -pGLO dish as the bacteria were not exposed to the antibiotic ampicillin. Furthermore, our group also expected to see inhibited bacterial growth in the LB/amp +pGLO dish as there was ampicillin in the dish, but some of the bacteria were immune as they possessed immunity to the ampicillin. Moreover, our group expected that there would be no bacterial growth in the LB/amp -pGLO dish, as the bacteria were exposed to ampicillin and were not immune. However, the final dish, LB/amp/arbo +pGLO, did not turn out as expected. While it was expected to allow for inhibited bacterial growth and the bacteria to become florescent,
The chemicals that Clorox bleach contains are water, sodium hypochlorite, sodium chloride, coco-betaine, fragrance, sodium carbonate, sodium chlorate, sodium hydroxide, sodium [olyacrylate, and sodium xylene sulfonate. Sodium hypochlorite has several short term potential health risks such as causing irritation and burns when in contact with skin, causing eye damage and irritation when in contact with eyes, irritating the nose, throat, and lungs upon inhalation, which can cause shortness of breath and pulmonary edema at higher exposures, and can also cause headache, dizziness, nausea, and vomiting. Chronic health effects include bronchitis after repeated exposure to inhalation. Fragrance can cause skin irritation, allergy, and has been linked to organ system toxicity. Sodium carbonate can be corrosive to the gastro intestinal tract if ingested. It can also cause blistering when in contact with skin and can be corrosive to eyes and cause conjuctival edema and corneal destruction. Sodium chlorate can irritate and burn skin and eyes, and can cause nausea, vomiting, and abdominal pain, as well as damage the kidneys and affect the liver. High levels of sodium chlorate can reduce the body’s ability to transport oxygen and at very high levels, exposure can lead to death. Sodium hydroxide is corrosive to skin and
The bacteria produce a number of products including urease and vacuolating cytotoxin (VacA). VacA damages endosomal and lysosomal membranes and leads to cell death. Urease helps increase the environmental pH to become less
THE EFFECT OF INCREASING THE CONCENTRATION OF DETTOL ANTISEPTIC LIQUID ON THE GROWTH OF E.COLI
The Zn²⁺ ion forms a ternary 1:1:1 antibiotic–metal–lipid complex. This means that the divalent metal ion complex can tightly bind to the lipid C₅₅-isoprenyl pyrophosphate molecules of the cell, acting like a bridge between the pyrophosphate and bacitracin zinc. Once the C₅₅-isoprenyl pyrophosphate has been compromised due to its inability to dephosphorylate or remove its phosphate (PO43−) through the pyrophosphatase enzyme and hydrolysis process, the pyrophosphate can no longer transport lipids into the cell-wall. Subsequently, this inhibits the process of cell-wall synthesis and results in the weakening of the cell wall and ultimately leads to bacterial
12. costen, P. (1997). Not Science Support from chlorine and dioxin combating trial design materials.
Any chemical thrown into the septic tank system can kill beneficial bacteria if too much is discarded into the tank.
Biocides are microorganisms or chemicals that can nullify the dangerous effects of harmful organisms. In lower concentrations of a biocide like triclosan, this synthetic broad spectrum chemical has bacteriostatic effects by inhibiting the growth of bacteria. At higher concentrations triclosan can exert bactericidal effects by acting as a disinfectant and antiseptic [3], [4]. Due to its non-specific anti-bacterial properties, this biocide has been overused in toys, cosmetics, and medical devices [5]. Due to the prevalent usage of triclosan, the bacteria of today
The interaction of isothiazolone biocides with microbial cells proceeds via several sequential reactions. Initially, a biocide molecule reacts with a thiol group to form a disulphide linkage with the biocide. This structure then interacts with a second thiol group to establish a new disulphide linkage. Following this, the cell releases the biocide as a mercaptoacrylamide. The mercaptoacrylamide derivative of CMIT tautomerizes to a thioacyl chloride under physiological conditions. The thioacyl chloride is highly reactive and proceeds to interact with other cell macromolecules. The ability of CMIT to degrade to a thioacyl chloride might account for it’s much greater antimicrobial activity when compared to BIT or MIT. (Daniel, 1997)
For external use only, if bag busts dispose of it immediately. Prolonged skin exposure to the chemicals can cause dry and red skin. If the chemicals do get onto skin wash the area with soap and water, then contact a physician. Do not inhale the chemicals, calcium chloride can create lesions in the mucous membranes that line the nose, throat, and lungs if inhaled regularly. If you begin coughing or have a sore throat, the IPCS recommends moving into a well-ventilated area and resting until your symptoms subside. If symptoms persist, see your doctor.
As the leader of this clan, I chose to bring chlorine because I believe the chemical properties will be important as a wound disinfectant and to help purify water. The first important chemical property of chlorine is it’s solution when mixed with water to help kill bacteria in a wound. According to a website called https://www.leaf.tv/articles/the-effects-of-pool-chlorine-on-healing-wounds/ “When chlorine in the water penetrates the skin, it will help kill the bacteria in the wound.” This property is very important on the island for any medical issues that might occur while on the island. Even though, we hope for no medical issues to arise there is always the possibility and we want to ensure that, that has the littlest possible chance of happening,
The results of this experiment revealed that with regards to viability, the chemicals infected and killed off more thymocytes than splenocytes. Considering that they thymus has more lymphocytes than the spleen, the researchers concluded that the chemicals had a really bad effect on the whales which is why the whales’ tissue were not healing because they were completely killed by these chemicals. While the results for the cell’s proliferation were not as bad in thymocytes as they were in splenocytes. The splenocytes could not make much of themselves as they normal would, their ability to divide was inhibited by the chemicals. The results were slightly different for each of the three organochlorines. Some of the chemicals did nothing but slow proliferation without actually killing off the cells while others did kill the
The analysis of the results by single type of mild stress (first analysis) as well as the analysis of the interactions (second analysis) indicates that the exposure to the OCC did not have a negative effect on the viability of Leuconostoc mesenteroides. In all the analysis performed by single type of mild stress (fist analysis) the same pattern was found; there were no significant differences between the negative and positive controls, low and medium levels of stress-agent within a given type of mild stress except for the highest levels of stress-agents which were significantly detrimental. In acid mild stress the survivability of Leuconostoc mesenteroides ranged from 103-63% the highest being the positive control and the lowest being exposure to pH 4.0 (Figure 4.1). Figure 4.2 shows that with the heat mild treatment no significant differences were found among both controls and all levels of mild heat stress (P > 0.05). The survivability ranged from 105- 100% (Figure 4.2). With ethanol, the survivability of Leuconostoc mesenteroides in the OCC was only different when exposed to 15% ethanol (v/v) (P < 0.05). The highest viability was obtained with the positive control which was 106% and the lowest with the 15% ethanol (v/v) with was 62%. (Figure 4.3). With the prior exposure to the oxidative stress, the viability in the OCC raged from 106-80% the highest being the positive control and the lowest being the 7.5 mM concentration of hydrogen peroxide which
] Some of non-toxic inorganic anions involving Br-, Cl-, NO3-SO42-, PO43-; non-toxic organic anions including acetate, benzoate, malate, glycolate, citrate and so forth.[2] Couling et al. reported that the toxicity of cation types were decreased in the order of tetrazolium, triazolium, imidazolium, pyridinium and ammonium.[4] In addition, there is a strong correlation between IL