Essay on Horseradish Peroxidase

1933 WordsMay 5, 20138 Pages
Effects of Temperature, PH, boiling and concentration on Horseradish Peroxidase ABSTRACT The purpose of this report is to find out the effect of change in the Temperature, PH, boiling, concentration in peroxidase activity. Peroxidase is an enzyme that converts toxic hydrogen peroxide (H2O2) into water and another harmless compound. In this experiment we use, turnips and horseradish roots which are rich in the peroxidase to study the activity of this enzyme. The activity of peroxidase with change in temperature was highest at 320 Celsius and lowest at 40C. The activity of peroxidase was highest at a pH of 7, while it was lowest at pH of 9.Peroxidase activity was very low and constant with boiled extract, while the activity was moderate…show more content…
Turnips and horse radish roots are rich source of this enzyme. In this experiment, we would carry out a reaction between hydrogen peroxide and guaiacol which is colorless dye, using peroxidase as a catalyst, to produce water and an oxidized form of guaiacol which is brown. The formation of brown color would serve as an indicator that the breakdown of Hydrogen Peroxide took place. The enzyme activity would be directly proportional to the brown color intensity. The color intensity would be measured using a spectrophotometer and standardized to find the corresponding concentration for each absorbance unit. MATERIALS Blender or mortar and pestle Fresh turnip or horseradish root Spectrophotometer curvettes 10 ml test tubes and rack 50ml beakers 5ml pipettes graduated in 0.1 ml units with suction devices or automatic pipetters and tips Thermometer METHOD As directed in the lab text book “BIOLOGICAL INVESTIGATIONS” RESULTS Time (sec) | Tube 2 and 340C | Tube 4 and 5 250C | Tube 6 and 7320C | Tube 8 and 9480C | 20 | 0.100 | 0.418 | 0.535 | 0.331 | 40 | 0.107 | 0.575 | 0.708 | 0.412 | 60 | 0.109 | 0.775 | 0.904 | 0.404 | 80 | 0.109 | 0.897 | 1.123 | 0.578 | 100 | 0.106 | 1.066 | 1.296 | 0.681 | 120 | 0.099 | 1.229 | 1.436 | 0.787 | Table 1: Temperature effects on Peroxidase Activity (absorbance at 500nm) Figure 1 From Figure 1 above, the optimal temperature for peroxidase activity
Open Document