Hot Sauces Affecting Earthworms

The dependent variable is pulsation rates of L. variegatus before and after they were in the treatments. The standardized variable of this experiment would be the temperature of the surroundings of the L. variegatus, the three pulsation rates taken for each worm before and after the treatments, and also the amount of time each worm was kept in their respective treatments. The level of treatment for this experiment would be ten because of the six different concentration treatments of caffeine and nicotine along with the four household drugs. The sample size of the experiment differed from some treatments to other. For the three different concentrations of caffeine and nicotine, the sample size was 18 black worms each. The sample size of the control treatment of week 1 was 6 black worms. 12 black worms were used for the control of week 2, decaffeinated coffee and instant coffee. 11 black worms were used for the tea treatment and 15 were used for the tobacco treatment. There were three replications of the pulsation rate readings per worm before and after the treatment. With all this information we were able to get the results we got.

In the experiment we used Turnip, Hydrogen Peroxide, Distilled Water, and Guaiacol as my substances. On the first activity, Effect of Enzyme concentration of Reaction Rate for low enzyme concentration, we tested three concentrations of the turnip extract, and hydrogen peroxide. For the Turnip Extract I used 0.5 ml, 1.0 ml, and 2.0 ml. For hydrogen peroxide we used 0.1 ml, 0.2 ml, and 0.4 ml. We used a control to see the standard, and used a control for each enzyme concentration used. The control contains turnip extract and the color reagent, Guaiacol. We prepared my substrate tubes separately from the enzyme tubes. My substrate tube

The topic of this lab is on biochemistry.This experiment was conducted to show how cells prevent the build of hydrogen peroxide in tissues. My group consisted of Lekha, Ruth, and Jason. There were used two different concentrations of hydrogen peroxide through this experiment , 1.5% and 3%. By testing two different types it is easier to understand how the H2O2 and catalase react with one another. To do this both the yeast, which was our catalase, and H2O2 were mixed together in a beaker. Each concentration was tested out twice for more accurate results . 1.5% concentrated H2O2 had an average reaction rate of 10.5 seconds while 3% concentrated H2O2 had an average reaction rate of 7.5 seconds. From this experiment we learned that by increasing the concentration of H2O2 and chemically combining it with a catalase it will speed up the reaction. Enzymes speed up chemical reactions . The independent variable in this experiment was the concentration of the H2O2. Some key vocabulary words are Catalase, enzyme, hydrogen peroxide ( H2O2), and concentration.

The purpose of our experiment was to observe the behavior of the pill bugs. We were trying to find exactly whether the pill bugs would be attracted or repelled by the chemicals. The chemicals used were vinegar, water, salt solution and a sugar

1. For this experiment, what were the independent variable and the dependent variable? What were the constants?

The topic is: The cleanest cleaner. In this experiment, the scientists will be testing which brand of detergents cause a better cleaning effect on one stain on multiple white shirts. The independent variable in this experiment consists of the different detergents, while the dependent variable consists of the amount by which the stain faded (using the purple light scale), and or removed, others include the white t shirts, washing cycle, and drying cycle, staining agent, and cycle of drying and washing. The control variable in this experiment will be the 1 trial run with water only per experiment. The unit while measuring the amount of detergent is mL, and a scale which the is going to use to measure the amount of stain faded.

Lab six requires students to observe the effects of pH and enzyme concentration on catecholase activity. Enzymes are organic catalysts that can affect the rate of a chemical reaction depending on the pH level and the concentration of the enzyme. As pH comes closer to a neutral pH the enzyme is at its greatest effectiveness. Also at the absorbance of a slope of 0.0122 the enzyme is affected greatly. The pH effect on enzymes can be tested by trying each pH level with a pH buffer of the same pH as labeled as the test tube and 1mL of potato juice, water, and catechol. This is all mixed together and put in the spectrophotometer to test how much is being absorbed at 420nm. As the effect on enzyme concentration can be tested almost the same way. This part of the exercise uses different amounts of pH 7-phosphate buffer and potato juice, and 1mL of catechol mixed together in a test tube. Each substance is put in the spectrophotometer at a wavelength set tot 420nm. The results are put down for every minute up to six minutes to see how enzyme concentration affects reaction rate. The results show that the pH 8 (0.494) affects the enzyme more than a pH of 4 (0.249), 6 (0.371), 7 (0.456), and 10 (0.126). Also the absorbance is greatest at a slope of 0.0122 with test tube C that has more effect on the reaction rate, than test tube A, B, and D.

There were three test tubes in which the experiment was held. A relatively equal sized portion of raw potato (this contained the enzyme [a biological catalyst] hydrogen peroxidase) was placed in each tube. Then, enough water to cover the potato was added. Proceeding this, each of the test tubes were assigned a temperature; cold, room temperature or warm (this was written on the tag so that they were not confused). The test tube destinated ‘cold’ was placed in a ice bath for five minutes. At the same time, the ‘hot’ test tube was placed in a hot water bath for five minutes. Meanwhile, the room temperature test tube sat at room temperature for five minutes. When the five minutes were over, the test tubes were returned to the rack (so that they were able to be observed). Then, the test tubes were allowed to sit at room temperature for five more minutes. Once that period of time was over, 2 ml of hydrogen peroxide (the substrate) was added to each tube.

The research question for the Alka Seltzer Lab is, “What is the effect of different surface areas of an Alka Seltzer tablets reaction time?’. Inside of an Alka Seltzer tablet there are two key ingredients; which are citric acid and baking soda. When a tablet is dropped into a liquid, the acid and baking soda show a physical reaction of fizzing and eventually dissolving. Materials needed for this lab was goggles, aprons, beakers, water of the same temperature, mortar pestle, and three alka seltzer tablets. In each beaker there was 150 mL of water. The first tablet was a full tablet, the second was broken into quarters, and the last tablet was crushed in a mortar pestle. The dependent variable of the lab was the rate of reaction, and the independent

Are pennies better clean or dirty? Clean, of course. Clean pennies are probably used more than dirty pennies, but maybe just clean all the dirty pennies with the products: orange juice, coca cola, and taco sauce and see which one cleans the pennies the best, so then just use those. If any of these products cleans pennies, then coca cola will clean the pennies the best. For independent variable it was the products and the dependent variable is the clean pennies. The control group is the coca cola and the experimental group is orange juice, coca cola, and the taco sauce. For this experiment there was only a few steps to do in order to have clean pennies. After all the materials are ready then pour the products into cups and put at least two pennies in each product. Once the products are in the cups then set timer for 30 minutes and repeat this step for three trials. Collect the weight of the pennies after 30 minutes each time and write it down to see which of the products took off most of the dirt on the penny. Collect all the other data that is needed. This is just one way that cleans pennies but

Higher levels of solution should produce higher levels of product. The independent variable for the control group data and the experiment data is the yeast concentration. The dependent variable for the control group data and the experiment data is how much oxygen is produced. The Constant for the control group data and the experiment data is time and amount of hydrogen peroxide. The products of the experiment will increase if the levels of reactants increase. Denatured yeast may cause change in the reaction of the experiment. For all trials of the control group, the concentration of yeast is 6ml. For the experiment data, the yeast concentration varies from 8mL, 10 mL, 12 mL, 14 mL, and 16 mL. The temperature may cause change in the reaction of the combination of yeast and hydrogen peroxide

This lab was to see chemical reactions with different liquids. This was important to find the unknown substance. The controlled variable was the unknown powder, since it doesn’t change. The independent variable was the liquids. These liquids were vinegar, iodine, and water. Lastly, the dependent variable is the chemical reactions. The reaction was affected by the different liquids. If the liquids are changed then the chemical reactions will be different, because they have different properties, causing the atoms to bond differently.

Independent variable: We will change the amount of drops of Hydrochloric acid added to the Amylase. ( 5 drops, 10 drops, 15 drops)

To find the effect of temperature on the activity of an enzyme, the experiment deals with the steps as follows. First, 3 mL if pH 7 phosphate buffer was used to fill three different test tubes that were labeled 10, 24, and 50. These three test tubes were set in three different temperature settings. The first test tube was placed in an ice-water bath for ten minutes until it reached a temperature of 2° C or less. The second tube’s temperature setting was at room temperature until a temperature of 21°C was reached. The third tube was placed in a beaker of warm-water until the contents of the beaker reached a temperature setting of 60° C. There were four more test tubes that were included in the procedure. Two of the test tubes contained potato juice were one was put in ice and the other was placed in warm-water. The other two test tubes contained catechol. One test tube was put in ice and the other in warm water. After

The experiment that the class worked on was about peroxidase. Peroxidase is part of the enzyme group that presents most living organisms (Ahmed, 2013). Peroxidase interferes with the removal of hydrogen peroxide (Ahmed, 2013). Hydrogen peroxide is a toxic product that have normal metabolism before it causes any cell damages (Ahmed, 2013). Peroxidase has two substrate and both of them must present a reaction (Ahmed, 2013). One of the two substrate is H2O2 and other one just depends on the organism or the cell type (Ahmed, 2013). The substrate that the class uses is turnip extract. In the class there were five experiments to do but the class were assigned into groups and each group were going to do two experiment. The names of the experiments are: Baseline, Temperature, and pH.