How Buffers Work And The Buffering Capacities Of Different Buffering Solutions

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pH and Buffers 10/1-10/2 Samuel Pellot Masaki Aerts, John McAndrew, Julien Panepinto 10/13/15 Biology 2 Saint Peter’s Prep Purpose The purpose of the lab was to find out how buffers work and the buffering capacities of different buffering solutions. Hypothesis If an acid is added to a substance then the pH level will drop because you are adding more acid (HCl) to the solute becomes more acidic. While adding a base to a substance will cause the pH level to rise because adding more base (NaOH) to the solute would make it more basic. Materials pH probe and computer connectors goggles aprons 250-mL beaker of fresh distilled water 50-mL test beakers (4) Spare 250-mL beaker of distilled water 0.1 M HCl in erlenmeyer flask 0.1 M NaOH in erlenmeyer flask 2 disposable pipettes (one for each erlenmeyer flask) 25-mL graduated cylinder Various biological organisms (or parts of an organism) such as yeast, potato, orange juice Various non-biological materials, such as, buffer, carbonated water, salt water, Alka-seltzer Various simple biological materials such as milk, gelatin, starch, grape juice Google Spreadsheets Safety Considerations When handling the Acid or Base to avoid getting any substance in eyes: Use protective eyewear While using any acid or base don 't drink or eat any substances while working with substances. To avoid getting any acid or base on your clothes handle substance with extreme care and wear an apron. If

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