How Buffers Work And The Buffering Capacities Of Different Buffering Solutions

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pH and Buffers
10/1-10/2

Samuel Pellot
Masaki Aerts, John McAndrew, Julien Panepinto
10/13/15
Biology 2
Saint Peter’s Prep
Purpose

The purpose of the lab was to find out how buffers work and the buffering capacities of different buffering solutions. Hypothesis

If an acid is added to a substance then the pH level will drop because you are adding more acid (HCl) to the solute becomes more acidic. While adding a base to a substance will cause the pH level to rise because adding more base (NaOH) to the solute would make it more basic.

Materials

pH probe and computer connectors goggles aprons
250-mL beaker of fresh distilled water
50-mL test beakers (4)
Spare 250-mL beaker of distilled water
0.1 M HCl in erlenmeyer flask
0.1 M NaOH in erlenmeyer flask
2 disposable pipettes (one for each erlenmeyer flask)
25-mL graduated cylinder
Various biological organisms (or parts of an organism) such as yeast, potato, orange juice
Various non-biological materials, such as, buffer, carbonated water, salt water, Alka-seltzer
Various simple biological materials such as milk, gelatin, starch, grape juice
Google Spreadsheets

Safety Considerations
When handling the Acid or Base to avoid getting any substance in eyes: Use protective eyewear
While using any acid or base don 't drink or eat any substances while working with substances.
To avoid getting any acid or base on your clothes handle substance with extreme care and wear an apron.
If

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