How Different Factors Affect Enzymatic Reactions

2660 WordsApr 17, 201611 Pages
Introduction This experiment was used to explore and show how different factors affect enzymatic reactions. A catalase enzyme was combined with hydrogen peroxide under different conditions and was judged on a scale of one through five based on how quickly the enzyme reacted. Also, red and yellow beads, representing substrates and other molecules respectively, were pulled out of a plastic bin to show the relationship between an enzyme and its substrate concentration. Enzymes are binding proteins that act as organic catalysts to speed up very slow organic reactions and lower activation energy. The reactant is called the substrate, and in this lab, it is represented by hydrogen peroxide. The way that an enzyme binds with its substrate can be…show more content…
With catalase enzymes, it turns something extremely poisonous into two non-toxic compounds through hydrolysis (the process in which a polymer is broken into monomers). (2H2O22H2O+O2). This particular enzyme is found in the tissues of plants and animals, and different factors such as temperature and substrate concentration directly relate to the rate of a reaction. Different substrate concentrations vary the rate of a reaction. The affinity of an enzyme for its substrate is told by its Km. A large Km shows very little attractiveness to the substrate while a small Km shows a very strong attractiveness. By calculating the Km value, the rate in response to the substrate concentrations is able to be calculated. Several factors affect the rate of an enzymatic reaction. This includes temperature and substrate concentration. Normally, increasing temperature increases the rate of reaction; however, when the environment gets too hot, the enzyme will begin to denature (the breakdown of a protein out of its 3 dimensional shape. When the enzyme loses its shape, it also loses its function.) Also, substrate concentration has a direct relationship with an enzymatic reaction. Generally, the greater the substrate concentration the higher rate of a reaction until it reaches its maximum, then it will remain at the constant rate. Methods and
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